PMID- 36569206
OWN - NLM
STAT- MEDLINE
DCOM- 20221227
LR  - 20230110
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 12
DP  - 2022
TI  - Localization and potential role of prostate microbiota.
PG  - 1048319
LID - 10.3389/fcimb.2022.1048319 [doi]
LID - 1048319
AB  - INTRODUCTION: We aimed to clarify the presence and localization of the prostate 
      microbiota and examine its association with benign prostate enlargement (BPE). 
      METHODS: The microbiota of prostate tissues and catheterized urine from 15 
      patients were analyzed by 16S metagenomic analysis and compared to show that the 
      prostate microbiota was not a contaminant of the urinary microbiota. Fluorescence 
      in situ hybridization (FISH) and in situ hybridization (ISH) using the specific 
      probe for eubacteria was performed on prostate tissue to show the localization of 
      bacteria in the prostate. The BPE group was defined as prostate volume >/=30 mL, 
      and the non-BPE group as prostate volume <30 mL. The microbiota of the two groups 
      were compared to clarify the association between prostate microbiota and BPE. 
      RESULTS: Faith's phylogenetic diversity index of prostate tissue was 
      significantly higher than that of urine (42.3+/-3.8 vs 25.5+/-5.6, P=0.01). Principal 
      coordinate analysis showed a significant difference between the microbiota of 
      prostate tissue and catheterized urine (P<0.01). FISH and ISH showed the presence 
      of bacteria in the prostatic duct. Comparison of prostate microbiota between the 
      BPE and non-BPE groups showed that the Chao1 index of the BPE group was 
      significantly lower than that of the latter [142 (50-316) vs 169 (97-665), 
      P=0.047] and the abundance of Burkholderia was significantly higher in the BPE 
      group than in the latter. CONCLUSIONS: We demonstrated that the prostate 
      microbiota was located in the prostatic duct and reduced diversity of prostate 
      microbiota was associated with BPE, suggesting that prostate microbiota plays a 
      role in BPE.
CI  - Copyright (c) 2022 Okada, Takezawa, Tsujimura, Imanaka, Kuribayashi, Ueda, Hatano, 
      Fukuhara, Kiuchi, Fujita, Motooka, Nakamura, Koyama, Shimada and Nonomura.
FAU - Okada, Koichi
AU  - Okada K
AD  - Department of Urology, Osaka University of Graduate School of Medicine, Suita, 
      Japan.
FAU - Takezawa, Kentaro
AU  - Takezawa K
AD  - Department of Urology, Osaka University of Graduate School of Medicine, Suita, 
      Japan.
FAU - Tsujimura, Go
AU  - Tsujimura G
AD  - Department of Urology, Osaka University of Graduate School of Medicine, Suita, 
      Japan.
FAU - Imanaka, Takahiro
AU  - Imanaka T
AD  - Department of Urology, Osaka University of Graduate School of Medicine, Suita, 
      Japan.
FAU - Kuribayashi, Sohei
AU  - Kuribayashi S
AD  - Department of Urology, Osaka University of Graduate School of Medicine, Suita, 
      Japan.
FAU - Ueda, Norichika
AU  - Ueda N
AD  - Department of Urology, Osaka University of Graduate School of Medicine, Suita, 
      Japan.
FAU - Hatano, Koji
AU  - Hatano K
AD  - Department of Urology, Osaka University of Graduate School of Medicine, Suita, 
      Japan.
FAU - Fukuhara, Shinichiro
AU  - Fukuhara S
AD  - Department of Urology, Osaka University of Graduate School of Medicine, Suita, 
      Japan.
FAU - Kiuchi, Hiroshi
AU  - Kiuchi H
AD  - Department of Urology, Osaka University of Graduate School of Medicine, Suita, 
      Japan.
FAU - Fujita, Kazutoshi
AU  - Fujita K
AD  - Department of Urology, Faculty of Medicine, Kindai University Hospital, 
      Osakasayama, Japan.
FAU - Motooka, Daisuke
AU  - Motooka D
AD  - Department of Infection Metagenomics, Genome Information Research Center, Osaka 
      University Research Institute for Microbial Diseases, Suita, Japan.
FAU - Nakamura, Shota
AU  - Nakamura S
AD  - Department of Infection Metagenomics, Genome Information Research Center, Osaka 
      University Research Institute for Microbial Diseases, Suita, Japan.
FAU - Koyama, Yoshihisa
AU  - Koyama Y
AD  - Department of Neuroscience and Cell Biology, Osaka University Graduate School of 
      Medicine, Suita, Japan.
FAU - Shimada, Shoichi
AU  - Shimada S
AD  - Department of Neuroscience and Cell Biology, Osaka University Graduate School of 
      Medicine, Suita, Japan.
FAU - Nonomura, Norio
AU  - Nonomura N
AD  - Department of Urology, Osaka University of Graduate School of Medicine, Suita, 
      Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221207
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
SB  - IM
MH  - Humans
MH  - Male
MH  - *Prostate
MH  - In Situ Hybridization, Fluorescence
MH  - Phylogeny
MH  - *Prostatic Hyperplasia/complications
PMC - PMC9768196
OTO - NOTNLM
OT  - beneign prostate hyperplasia
OT  - benign prostate enlargement
OT  - in situ hybridization
OT  - microbiome
OT  - microbiota
OT  - prostate
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/12/27 06:00
MHDA- 2022/12/28 06:00
PMCR- 2022/01/01
CRDT- 2022/12/26 04:08
PHST- 2022/09/19 00:00 [received]
PHST- 2022/11/09 00:00 [accepted]
PHST- 2022/12/26 04:08 [entrez]
PHST- 2022/12/27 06:00 [pubmed]
PHST- 2022/12/28 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2022.1048319 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2022 Dec 7;12:1048319. doi: 
      10.3389/fcimb.2022.1048319. eCollection 2022.