PMID- 36570020
OWN - NLM
STAT- MEDLINE
DCOM- 20221227
LR  - 20221230
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2022
DP  - 2022
TI  - MiR-181a-5p Delivered by Adipose-Derived Mesenchymal Stem Cell Exosomes 
      Alleviates Klebsiella pneumonia Infection-Induced Lung Injury by Targeting STAT3 
      Signaling.
PG  - 5188895
LID - 10.1155/2022/5188895 [doi]
LID - 5188895
AB  - BACKGROUND: Klebsiella pneumoniae (K. pneu) is a leading cause of gram-negative 
      pneumonia, which requires effective treatment. Adipose-derived mesenchymal stem 
      cell- (ADSC-) derived exosomal microRNAs (miRNAs) have presented the inhibitory 
      effect of multiple diseases. However, the function of ADSC-derived exosomal 
      miRNAs in K. pneu remains unclear. AIM: In this study, we aimed to explore the 
      effect of ADSC-derived exosomal miR-181-5p on K. pneu infection-induced lung 
      injury. METHODS: C57BL/6 mouse model was established by infection of K. pneu. 
      ADSCs and exosomes were extracted and characterized in vitro. The translocation 
      of ADSC-derived exosomes to bone marrow-derived macrophages (BMDMs) was detected. 
      The level of miR-181a-5p was detected by real-time PCR. The secretion of 
      inflammatory factors was determined by ELISA. The interaction between miR-181a-5p 
      with STAT3 was identified. RESULTS: We successfully isolated the ADSCs that 
      express positive markers CD90 and CD105 rather than CD31 and CD45. The exosomal 
      miR-181a-5p secreted by ADSCs were internalized by BMDM and K. pneu infection 
      stimulated the miR-181a-5p level in bronchoalveolar lavage fluid (BALF) and BMDM. 
      ADSC-derived exosomal miR-181a-5p repressed pulmonary outgrowth and dissemination 
      of K. pneu infection in mice, repressed cellular infiltration in lung tissue, and 
      attenuated the inflammasome activity and the levels of IL-1beta and IL-18 in the 
      lung. Mechanically, miR-181a-5p was able to inhibit STAT3 expression at 
      posttranscriptional levels and repressed Nlrp3 and Asc expression in BMDM. 
      CONCLUSION: Consequently, we concluded that ADSC-derived exosomal miR-181a-5p 
      alleviated Klebsiella pneumonia infection-induced lung injury by targeting STAT3 
      signaling. ADSC-derived exosomal miR-181a-5p may serve as a potential candidate 
      for the treatment of Klebsiella pneumonia infection-induced lung injury.
CI  - Copyright (c) 2022 Ren-Jing Hu et al.
FAU - Hu, Ren-Jing
AU  - Hu RJ
AD  - Department of Laboratory Medicine, Wuxi Second People's Hospital Affiliated to 
      Nanjing Medical University, Wuxi City, 214000 Jiangsu Province, China.
FAU - Chen, Xiao-Chun
AU  - Chen XC
AD  - Department of Laboratory Medicine, Taizhou Second People's Hospital, Taizhou 
      City, 225411 Jiangsu Province, China.
FAU - Xu, Lei
AU  - Xu L
AD  - Department of Oral and Maxillofacial Surgery, Wuxi Stomatological Hospital, Wuxi 
      City, 214001 Jiangsu Province, China.
FAU - Rui, Xiao-Hong
AU  - Rui XH
AD  - Department of Laboratory Medicine, Wuxi Fifth People's Hospital, Wuxi City, 
      214000 Jiangsu Province, China.
FAU - Wan, Lin
AU  - Wan L
AD  - Department of Laboratory Medicine, Wuxi Second People's Hospital Affiliated to 
      Nanjing Medical University, Wuxi City, 214000 Jiangsu Province, China.
FAU - Lu, Jie
AU  - Lu J
AD  - Department of Laboratory Medicine, Wuxi Second People's Hospital Affiliated to 
      Nanjing Medical University, Wuxi City, 214000 Jiangsu Province, China.
FAU - Liu, Jun
AU  - Liu J
AD  - Department of Laboratory Medicine, Wuxi Fifth People's Hospital, Wuxi City, 
      214000 Jiangsu Province, China.
FAU - Pei, Hao
AU  - Pei H
AUID- ORCID: 0000-0002-2422-8419
AD  - Department of Laboratory Medicine, Wuxi Fifth People's Hospital, Wuxi City, 
      214000 Jiangsu Province, China.
LA  - eng
PT  - Journal Article
DEP - 20221214
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Mice
MH  - Animals
MH  - Klebsiella pneumoniae/metabolism
MH  - *Exosomes/metabolism
MH  - *Lung Injury/metabolism
MH  - Mice, Inbred C57BL
MH  - *MicroRNAs/metabolism
MH  - *Mesenchymal Stem Cells/metabolism
MH  - *Pneumonia/metabolism
PMC - PMC9771653
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2022/12/27 06:00
MHDA- 2022/12/28 06:00
PMCR- 2022/12/14
CRDT- 2022/12/26 04:27
PHST- 2021/06/18 00:00 [received]
PHST- 2022/01/16 00:00 [revised]
PHST- 2022/01/27 00:00 [accepted]
PHST- 2022/12/26 04:27 [entrez]
PHST- 2022/12/27 06:00 [pubmed]
PHST- 2022/12/28 06:00 [medline]
PHST- 2022/12/14 00:00 [pmc-release]
AID - 10.1155/2022/5188895 [doi]
PST - epublish
SO  - Mediators Inflamm. 2022 Dec 14;2022:5188895. doi: 10.1155/2022/5188895. 
      eCollection 2022.