PMID- 36571220
OWN - NLM
STAT- MEDLINE
DCOM- 20221227
LR  - 20221230
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 47
IP  - 12
DP  - 2022 Dec 25
TI  - [Electroacupuncture promotes gastrointestinal motility by activating autophagy of 
      Cajal interstitial cells via downregulating PI3K/Akt/mTOR signaling pathway in 
      stomach of diabetic gastro-paresis rats].
PG  - 1060-7
LID - 10.13702/j.1000-0607.20211241 [doi]
AB  - OBJECTIVE: To observe the effect of electroacupuncture (EA) of "Zusanli" (ST36), 
      "Sanyinjiao" (SP6) and "Liangmen" (ST21) on gastrointestinal motility, blood 
      glucose content and expression of autophagy-related proteins 1 light chain 3 
      (LC3), p62, phosphatidyli-nositol-3 kinase (PI3K), protein kinase B (Akt), p-Akt 
      and mammalian target protein of rapamycin (mTOR) of interstitial cells of Cajal 
      (ICCs) in the cultured gastric antrum cells in diabetic gastroparesis (DGP) rats, 
      so as to reveal its mechanisms underlying improvement of DGP. METHODS: A total of 
      45 Sprague Dawley (SD) rats were randomly divided into blank control, model, EA, 
      medication (3-methyladenine, 3-MA) and EA+3-MA groups, with 9 rats in each group. 
      The DGP model was established by intraperitoneal injection of 2% streptozotocin 
      (STZ) combined with high-fat and high sugar diet for 8 weeks. The gastric 
      emptying rate was measured by using gavage of phenol red (to measure the 
      propelling length of the phenol red/total length of small intestine x100%). The 
      symptom score (mental state, coat color and luster, behavior and activity, stool 
      traits) of rats was observed every week and the blood glucose content was 
      measured by using a glucometer. EA (20 Hz/100 Hz, 2 mA) was applied to unilateral 
      ST36, SP6 and ST21 alternatively for 15 min, once daily, 5 days a week for 3 
      weeks. Rats of the 3-MA and 3-MA+EA groups received intraperitoneal injection of 
      3-MA (30 mg.kg(-1).d(-1), 10 mg/mL), once daily, 5 days a week for 3 weeks. After 
      15 days' intervention, the rats were operated for gastric emptying rate test, 
      specimen collection, isolation, and culture of primary ICCs. The expression 
      levels of microtubule associated protein LC3, p62, PI3K, Akt, p-Akt and mTOR of 
      ICCs of cultured gastric antrum cells were detected using Western blot, and the 
      number of autophagosomes in ICC of gastric antrum was observed under transmission 
      electron microscope. RESULTS: Compared with the blank control group, the symptom 
      score, blood glucose, and the expression levels of p62, class Ⅰ PI3K, Akt, p-Akt 
      and mTOR proteins were increased significantly (P<0.01), while the gastric 
      emptying rate and ratio of LC3Ⅱ/LC3Ⅰ and the expression level of class Ⅲ PI3K 
      protein were significantly decreased (P<0.05, P<0.01) in the model group. In 
      comparison with the model group, the increase of symptom score, blood glucose, 
      and expression levels of p62, class Ⅰ PI3K, Akt, p-Akt and mTOR proteins and the 
      decrease of gastric empty rate and LC3Ⅱ/LC3Ⅰ ratio and the expression level of 
      class Ⅲ PI3K protein were all reversed in both EA and EA+3-MA groups (P<0.05, 
      P<0.01), rather than in the 3-MA group. In addition, 3-MA also reversed 
      modeling-induced increase of class Ⅰ PI3K, Akt, p-Akt and mTOR proteins 
      expression (P<0.01). No significant differences were found between the EA and 
      EA+3-MA in downregulating the levels of symptom score and blood glucose content, 
      and in upregulating gastric empty rate(P>0.05). The effect of EA was notably 
      superior to that of EA+3-MA in upregulating the ratio of LC3Ⅱ/LC3Ⅰ and the 
      expression level of class Ⅲ PI3K protein, and in downregulating the expression of 
      p62, class Ⅰ PI3K, Akt, p-Akt and mTOR proteins (P<0.05, P<0.01). The findings of 
      transmission electron microscopy showed obvious swelling, breakage of some 
      mitochondrial cristae in the ICC cells of antrum and no autophagosomes in the 
      model group and 3-MA group, which was milder in the damage of mitochondrial 
      cristae and marked increase in the autophagosomes in both EA and EA+3-MA groups. 
      CONCLUSION: EA can improve the gastrointestinal motility and symptoms in DGP 
      rats, which may be related to its functions in downregulating PI3K/Akt/mTOR 
      signaling to promote autophagy level of ICC.
FAU - Zhang, Tian-Hua
AU  - Zhang TH
AD  - College of Acupuncture-Moxibustion-Tuina and Rehabilitation, Hunan University of 
      Chinese Medicine, Changsha 410208, China.
FAU - Zhao, Sha-Tong
AU  - Zhao ST
AD  - College of Acupuncture-Moxibustion-Tuina and Rehabilitation, Hunan University of 
      Chinese Medicine, Changsha 410208, China.
FAU - Li, Xiao-Yu
AU  - Li XY
AD  - College of Acupuncture-Moxibustion-Tuina and Rehabilitation, Hunan University of 
      Chinese Medicine, Changsha 410208, China.
FAU - Xiao, Xiao-Juan
AU  - Xiao XJ
AD  - College of Acupuncture-Moxibustion-Tuina and Rehabilitation, Hunan University of 
      Chinese Medicine, Changsha 410208, China.
FAU - Xiao, Le
AU  - Xiao L
AD  - College of Acupuncture-Moxibustion-Tuina and Rehabilitation, Hunan University of 
      Chinese Medicine, Changsha 410208, China.
FAU - Wei, Xing
AU  - Wei X
AD  - College of Acupuncture-Moxibustion-Tuina and Rehabilitation, Hunan University of 
      Chinese Medicine, Changsha 410208, China.
FAU - Peng, Yan
AU  - Peng Y
AD  - College of Acupuncture-Moxibustion-Tuina and Rehabilitation, Hunan University of 
      Chinese Medicine, Changsha 410208, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - 0 (Blood Glucose)
RN  - I6G9Y0J1OJ (Phenolsulfonphthalein)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.1.1 (mTOR protein, rat)
SB  - IM
MH  - Rats
MH  - Animals
MH  - Rats, Sprague-Dawley
MH  - Proto-Oncogene Proteins c-akt/genetics/metabolism
MH  - *Interstitial Cells of Cajal/metabolism
MH  - Phosphatidylinositol 3-Kinases/genetics
MH  - *Electroacupuncture
MH  - Blood Glucose/metabolism
MH  - Phenolsulfonphthalein/metabolism
MH  - *Gastroparesis/genetics/therapy/metabolism
MH  - Signal Transduction
MH  - Paresis/metabolism
MH  - Pyloric Antrum/metabolism
MH  - TOR Serine-Threonine Kinases/genetics
MH  - Autophagy
MH  - Gastrointestinal Motility
MH  - *Diabetic Neuropathies
MH  - Mammals/metabolism
OTO - NOTNLM
OT  - Autophagy
OT  - Cajal interstitial cells
OT  - Diabetic gastroparesis
OT  - Electroacupuncture
OT  - PI3K/Akt/mTOR signaling pathway
EDAT- 2022/12/27 06:00
MHDA- 2022/12/28 06:00
CRDT- 2022/12/26 05:33
PHST- 2022/12/26 05:33 [entrez]
PHST- 2022/12/27 06:00 [pubmed]
PHST- 2022/12/28 06:00 [medline]
AID - 10.13702/j.1000-0607.20211241 [doi]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2022 Dec 25;47(12):1060-7. doi: 10.13702/j.1000-0607.20211241.