PMID- 36571862
OWN - NLM
STAT- MEDLINE
DCOM- 20230214
LR  - 20230214
IS  - 1873-3557 (Electronic)
IS  - 1386-1425 (Linking)
VI  - 290
DP  - 2023 Apr 5
TI  - Colorimetric detection of biothiols and Hg(2+) based on the peroxidase-like 
      activity of GTP.
PG  - 122263
LID - S1386-1425(22)01411-1 [pii]
LID - 10.1016/j.saa.2022.122263 [doi]
AB  - Guanosine-5'-triphosphate (GTP) not only plays a key role in a majority of 
      cellular processes but also be proposed as a peroxidase-like mimic. Compared with 
      nanozymes, GTP shows good tolerance under harsh conditions, which can be used to 
      construct an easy colorimetric analysis for the detection of biomolecules. Here, 
      on the basis of the peroxidase-like activity of GTP which can catalyze the 
      oxidation of 3,3',5,5'-tetramethyl benzidine dihydrochloride (TMB), colorimetric 
      sensing was established for biothiols and Hg(2+). Biothiols reduced the oxTMB 
      back to colorless TMB, and Hg(2+) restored the formation of oxTMB, leading to the 
      recovery of color. This method not only provides a platform for the detection of 
      metal ions and biothiols, but also shows that GTP has great potential for 
      analytical detection.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Huang, Na
AU  - Huang N
AD  - College of Chemistry and Chemical Engineering, Southwest University, Beibei, 
      Chongqing 400715, China.
FAU - Yang, Dan
AU  - Yang D
AD  - College of Chemistry and Chemical Engineering, Southwest University, Beibei, 
      Chongqing 400715, China.
FAU - Chen, Huanhuan
AU  - Chen H
AD  - College of Chemistry and Chemical Engineering, Southwest University, Beibei, 
      Chongqing 400715, China.
FAU - Xiao, Yu
AU  - Xiao Y
AD  - College of Chemistry and Chemical Engineering, Southwest University, Beibei, 
      Chongqing 400715, China.
FAU - Wen, Jiahui
AU  - Wen J
AD  - College of Chemistry and Chemical Engineering, Southwest University, Beibei, 
      Chongqing 400715, China.
FAU - Long, Yijuan
AU  - Long Y
AD  - College of Chemistry and Chemical Engineering, Southwest University, Beibei, 
      Chongqing 400715, China.
FAU - Zheng, Huzhi
AU  - Zheng H
AD  - College of Chemistry and Chemical Engineering, Southwest University, Beibei, 
      Chongqing 400715, China. Electronic address: zhenghz@swu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20221220
PL  - England
TA  - Spectrochim Acta A Mol Biomol Spectrosc
JT  - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
JID - 9602533
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 1.11.1.- (Peroxidases)
RN  - EC 1.- (Oxidoreductases)
RN  - FXS1BY2PGL (Mercury)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - *Colorimetry/methods
MH  - Peroxidase
MH  - Peroxidases
MH  - Oxidoreductases
MH  - *Mercury
MH  - Hydrogen Peroxide
OTO - NOTNLM
OT  - Biothiols
OT  - GTP
OT  - Hg(2+)
OT  - Peroxidase-like activity
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/12/27 06:00
MHDA- 2023/02/15 06:00
CRDT- 2022/12/26 18:05
PHST- 2022/08/24 00:00 [received]
PHST- 2022/11/25 00:00 [revised]
PHST- 2022/12/17 00:00 [accepted]
PHST- 2022/12/27 06:00 [pubmed]
PHST- 2023/02/15 06:00 [medline]
PHST- 2022/12/26 18:05 [entrez]
AID - S1386-1425(22)01411-1 [pii]
AID - 10.1016/j.saa.2022.122263 [doi]
PST - ppublish
SO  - Spectrochim Acta A Mol Biomol Spectrosc. 2023 Apr 5;290:122263. doi: 
      10.1016/j.saa.2022.122263. Epub 2022 Dec 20.