PMID- 36572554
OWN - NLM
STAT- MEDLINE
DCOM- 20230201
LR  - 20230216
IS  - 1522-1490 (Electronic)
IS  - 0363-6119 (Linking)
VI  - 324
IP  - 2
DP  - 2023 Feb 1
TI  - Sodium oxamate reduces lactate production to improve the glucose homeostasis of 
      Micropterus salmoides fed high-carbohydrate diets.
PG  - R227-R241
LID - 10.1152/ajpregu.00226.2022 [doi]
AB  - The study was performed to evaluate the effects of the reduced lactate production 
      by sodium oxamate (SO) on growth performance, lactate and glucose and lipid 
      metabolism, and glucose tolerance of Micropterus salmoides fed high-carbohydrate 
      (CHO) diets. In in vitro study, primary hepatocytes were incubated for 48 h in a 
      control medium (5.5 mM glucose), a high-glucose medium (25 mM glucose, HG), or a 
      SO-containing high-glucose medium (25 mM glucose + 50 mM SO, HG-SO). Results 
      indicated lactate and triglyceride (TG) levels, and lactate dehydrogenase a 
      (LDH-a) expression in the HG-SO group were remarkably lower than those of the HG 
      group. In in vivo study, M. salmoides (5.23 +/- 0.03 g) were fed four diets 
      containing a control diet (10% CHO, C) and three SO contents [0 (HC), 100 
      (HC-SO1), and 200 (HC-SO2) mg.kg(-1), respectively] of high-CHO diets (20% CHO) 
      for 11 wk. High-CHO diets significantly reduced weight gain rate (WGR), specific 
      growth rate (SGR), p-AMPK-to-t-AMPK ratio, and expression of insulin receptor 
      substrate 1 (IRS1), insulin-like growth factor I (IGF-I), insulin-like growth 
      factor I receptor (IGF-IR), fructose-1,6-biphosphatase (FBPase), peroxisome 
      proliferator-activated receptor alpha (PPARalpha), and carnitine palmitoyl transferase 1alpha 
      (CPT1alpha) compared with the C group, whereas the opposite was true for plasma 
      levels of glucose, TG, lactate, tissue glycogen, and lipid contents, and 
      expression of LDH-a, monocarboxylate transporter 1 and 4 (MCT1 and MCT4), 
      insulin, glucokinase (GK), pyruvate dehydrogenase E1 subunit (PDH), 
      sterol-regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS). 
      The HC-SO2 diets remarkably increased WGR, SGR, p-AMPK-to-t-AMPK ratio, and 
      expression of IRS1, IGF-I, IGF-IR, GK, PDHalpha, PDHbeta, FAS, acetyl-CoA carboxylase 1 
      (ACC1), PPARalpha, and CPT1alpha compared with the HC group. Besides, HC-SO2 diets also 
      enhanced glucose tolerance of fish after a glucose loading. Overall, the reduced 
      lactate production by SO benefits growth performance and glucose homeostasis of 
      high-CHO-fed M. salmoides through the enhancement of glycolysis, lipogenesis, and 
      fatty acid beta-oxidation coupled with the suppression of glycogenesis and 
      gluconeogenesis.
FAU - Shen, Hui-Chao
AU  - Shen HC
AD  - College of Marine Sciences, South China Agricultural University, Guangzhou, 
      China.
AD  - University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on 
      Marine Bioresource Conservation and Exploitation, Guangzhou, China.
FAU - Chen, Zhi-Qiang
AU  - Chen ZQ
AD  - College of Marine Sciences, South China Agricultural University, Guangzhou, 
      China.
FAU - Liu, Xiao-Cheng
AU  - Liu XC
AD  - College of Marine Sciences, South China Agricultural University, Guangzhou, 
      China.
FAU - Guan, Jun-Feng
AU  - Guan JF
AD  - College of Marine Sciences, South China Agricultural University, Guangzhou, 
      China.
AD  - University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on 
      Marine Bioresource Conservation and Exploitation, Guangzhou, China.
FAU - Xie, Di-Zhi
AU  - Xie DZ
AD  - College of Marine Sciences, South China Agricultural University, Guangzhou, 
      China.
AD  - University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on 
      Marine Bioresource Conservation and Exploitation, Guangzhou, China.
FAU - Li, Yuan-You
AU  - Li YY
AD  - College of Marine Sciences, South China Agricultural University, Guangzhou, 
      China.
AD  - University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on 
      Marine Bioresource Conservation and Exploitation, Guangzhou, China.
FAU - Xu, Chao
AU  - Xu C
AUID- ORCID: 0000-0001-9943-8659
AD  - College of Marine Sciences, South China Agricultural University, Guangzhou, 
      China.
AD  - University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on 
      Marine Bioresource Conservation and Exploitation, Guangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221226
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 0 (PPAR alpha)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - EC 1.1.1.27.- (Lactate Dehydrogenase 5)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - *Insulin-Like Growth Factor I/metabolism
MH  - *Bass/metabolism
MH  - Lactic Acid/metabolism
MH  - PPAR alpha
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Lactate Dehydrogenase 5/metabolism/pharmacology
MH  - Diet
MH  - Glucose/metabolism
MH  - Homeostasis
MH  - Liver/metabolism
OTO - NOTNLM
OT  - carbohydrate utilization
OT  - fish culture
OT  - lactate metabolism
OT  - sodium oxamate
EDAT- 2022/12/27 06:00
MHDA- 2023/02/02 06:00
CRDT- 2022/12/26 21:42
PHST- 2022/12/27 06:00 [pubmed]
PHST- 2023/02/02 06:00 [medline]
PHST- 2022/12/26 21:42 [entrez]
AID - 10.1152/ajpregu.00226.2022 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2023 Feb 1;324(2):R227-R241. doi: 
      10.1152/ajpregu.00226.2022. Epub 2022 Dec 26.