PMID- 36574861
OWN - NLM
STAT- MEDLINE
DCOM- 20230120
LR  - 20230503
IS  - 1873-5169 (Electronic)
IS  - 0196-9781 (Linking)
VI  - 160
DP  - 2023 Feb
TI  - Short-term depletion of plasma estrogen affects hypothalamic 
      kisspeptin-neurokinin B-dynorphin A neurons, gonadotrophs, and pulsatile 
      luteinizing hormone secretion in female rats.
PG  - 170929
LID - S0196-9781(22)00195-4 [pii]
LID - 10.1016/j.peptides.2022.170929 [doi]
AB  - Kisspeptin-neurokinin B-dynorphin A (KNDy) neurons in the arcuate nucleus (ARC) 
      regulate pulsatile luteinizing hormone (LH) secretion. These neurons express 
      estrogen receptors and are negatively regulated by estrogen. This study aimed to 
      determine whether estrogen supplementation after short-term ovariectomy-induced 
      estrogen depletion has different effects on KNDy neurons depending on the timing 
      of the supplementation. To decrease endogenous estradiol (E2) for a short time, 
      adult female rats received a tube filled with E2 one week after ovariectomy and 
      utilized it one week later (O1w + E). From the results of immunohistochemistry, 
      the response to E2 was attenuated in KNDy neurons of O1w + E rats. Enlarged 
      LH-secreting cells in the anterior pituitary were found in O1w + E rats; however, 
      such enlarged LH cells were not found in ones without previous short-term E2 
      depletion. From the analysis of LH pulses, plasma LH levels were increased in O1w 
      + E rats relative to ones without previous short-term E2 depletion. These results 
      suggested that once endogenous sex steroids were depleted, the response to E2 in 
      hypothalamic KNDy neurons did not fully recover in one week. Thus, short-term sex 
      steroid depletion due to gonadectomy could alter the response to the sex steroids 
      in KNDy neurons even though the period without sex steroids is only one week, and 
      the alteration is likely to affect plasma hormone levels.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Iwata, Kinuyo
AU  - Iwata K
AD  - Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon 
      Medical School, Tokyo 113-8602, Japan. Electronic address: kiwata0309@nms.ac.jp.
FAU - Ogata, Risako
AU  - Ogata R
AD  - Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon 
      Medical School, Tokyo 113-8602, Japan.
FAU - Sato, Marimo
AU  - Sato M
AD  - Department of Veterinary Medical Sciences, The University of Tokyo, Tokyo 
      113-8657, Japan.
FAU - Matsuda, Fuko
AU  - Matsuda F
AD  - Department of Veterinary Medical Sciences, The University of Tokyo, Tokyo 
      113-8657, Japan.
FAU - Ishii, Hirotaka
AU  - Ishii H
AD  - Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon 
      Medical School, Tokyo 113-8602, Japan.
FAU - Ozawa, Hitoshi
AU  - Ozawa H
AD  - Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon 
      Medical School, Tokyo 113-8602, Japan; Faculty of Health Science, Bukkyo 
      Univeristy, Kyoto, 604-8418, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221224
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 86933-75-7 (Neurokinin B)
RN  - 74913-18-1 (Dynorphins)
RN  - 0 (Kisspeptins)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - 0 (Estrogens)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
SB  - IM
MH  - Rats
MH  - Female
MH  - Animals
MH  - *Neurokinin B/metabolism
MH  - Dynorphins/metabolism
MH  - *Gonadotrophs/metabolism
MH  - Kisspeptins/metabolism
MH  - Luteinizing Hormone
MH  - Estrogens
MH  - Arcuate Nucleus of Hypothalamus
MH  - Neurons/metabolism
MH  - Gonadotropin-Releasing Hormone
OTO - NOTNLM
OT  - Arcuate nucleus
OT  - Estradiol treatment
OT  - Gonadotropin
OT  - Kiss1
OT  - Metastin
OT  - Sex steroid hormone
COIS- Declaration of Competing Interest The authors have no competing interests to 
      declare.
EDAT- 2022/12/28 06:00
MHDA- 2023/01/21 06:00
CRDT- 2022/12/27 19:11
PHST- 2022/09/29 00:00 [received]
PHST- 2022/12/04 00:00 [revised]
PHST- 2022/12/23 00:00 [accepted]
PHST- 2022/12/28 06:00 [pubmed]
PHST- 2023/01/21 06:00 [medline]
PHST- 2022/12/27 19:11 [entrez]
AID - S0196-9781(22)00195-4 [pii]
AID - 10.1016/j.peptides.2022.170929 [doi]
PST - ppublish
SO  - Peptides. 2023 Feb;160:170929. doi: 10.1016/j.peptides.2022.170929. Epub 2022 Dec 
      24.