PMID- 36576716
OWN - NLM
STAT- MEDLINE
DCOM- 20230616
LR  - 20230620
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 478
IP  - 8
DP  - 2023 Aug
TI  - Pathological alterations in the expression status of rotator cuff tendon matrix 
      components in hyperlipidemia.
PG  - 1887-1898
LID - 10.1007/s11010-022-04643-6 [doi]
AB  - Hyperlipidemia is an important risk factor in the development and progression of 
      tendon pathology, however its role in aggravating rotator cuff tendon injury 
      (RCTI) is largely unknown. We aimed to assess the expression status of key 
      extracellular matrix (ECM) components in the tendon tissues and tenocytes under 
      hyperlipidemia. Shoulder rotator cuff (RC) tendon tissues harvested from the 
      swine model of hyperlipidemia displayed alterations in histomorphometry and the 
      expression status of major ECM component proteins including COL-I, COL-III, 
      COL-IV, COL-V, COL-VI, MMP2, and MMP9. Similarly, the LDL- and oxLDL-challenged 
      tenocytes displayed altered expression of the same proteins at both 
      transcriptional and translational levels. In addition, the lipid uptake and 
      cellular reactive oxygen radicals predominated in the lipid-challenged tenocytes 
      compared to the control. Overall, the LDL-treated cells displayed predominant 
      pathological alterations compared to the ox-LDL-treated cells. Further 
      understanding regarding the underlying molecular mechanisms driving the tendon 
      matrisome alteration and subsequent aggravated RCTI pathology in hyperlipidemia 
      could open novel translational avenues in the management of RCTI.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Fang, William
AU  - Fang W
AUID- ORCID: 0000-0003-1703-6969
AD  - Department of Translational Research, College of Osteopathic Medicine of the 
      Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 
      91766, USA.
FAU - Sekhon, Seerat
AU  - Sekhon S
AD  - Department of Translational Research, College of Osteopathic Medicine of the 
      Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 
      91766, USA.
FAU - Teramoto, Darren
AU  - Teramoto D
AD  - Department of Translational Research, College of Osteopathic Medicine of the 
      Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 
      91766, USA.
FAU - Fung, Cameron
AU  - Fung C
AD  - Department of Translational Research, College of Osteopathic Medicine of the 
      Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 
      91766, USA.
FAU - La, Vy
AU  - La V
AD  - Department of Translational Research, College of Osteopathic Medicine of the 
      Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 
      91766, USA.
FAU - Duong, Cindy
AU  - Duong C
AD  - Department of Translational Research, College of Osteopathic Medicine of the 
      Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 
      91766, USA.
FAU - Doescher, Christian
AU  - Doescher C
AD  - Department of Translational Research, College of Osteopathic Medicine of the 
      Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 
      91766, USA.
FAU - Thai, An
AU  - Thai A
AD  - Department of Translational Research, College of Osteopathic Medicine of the 
      Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 
      91766, USA.
FAU - Thankam, Finosh G
AU  - Thankam FG
AUID- ORCID: 0000-0001-7285-9808
AD  - Department of Translational Research, College of Osteopathic Medicine of the 
      Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 
      91766, USA.
FAU - Agrawal, Devendra K
AU  - Agrawal DK
AUID- ORCID: 0000-0001-5445-0013
AD  - Department of Translational Research, College of Osteopathic Medicine of the 
      Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 
      91766, USA. DAgrawal@WesternU.edu.
LA  - eng
GR  - R01 HL144125/HL/NHLBI NIH HHS/United States
GR  - R01 HL144125/NH/NIH HHS/United States
PT  - Journal Article
DEP - 20221228
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Lipids)
SB  - IM
MH  - Swine
MH  - Animals
MH  - Rotator Cuff/metabolism
MH  - *Hyperlipidemias/metabolism
MH  - Tendons/metabolism/pathology
MH  - *Rotator Cuff Injuries/genetics/metabolism/pathology
MH  - Extracellular Matrix Proteins/metabolism
MH  - Lipids
OTO - NOTNLM
OT  - Collagen homeostasis
OT  - ECM disorganization
OT  - Hypercholesterolemia
OT  - Hyperlipidemia
OT  - Rotator cuff tendon injury
EDAT- 2022/12/29 06:00
MHDA- 2023/06/16 06:42
CRDT- 2022/12/28 11:21
PHST- 2022/11/02 00:00 [received]
PHST- 2022/12/13 00:00 [accepted]
PHST- 2023/06/16 06:42 [medline]
PHST- 2022/12/29 06:00 [pubmed]
PHST- 2022/12/28 11:21 [entrez]
AID - 10.1007/s11010-022-04643-6 [pii]
AID - 10.1007/s11010-022-04643-6 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2023 Aug;478(8):1887-1898. doi: 10.1007/s11010-022-04643-6. 
      Epub 2022 Dec 28.