PMID- 36579614
OWN - NLM
STAT- MEDLINE
DCOM- 20230314
LR  - 20230316
IS  - 1365-2958 (Electronic)
IS  - 0950-382X (Linking)
VI  - 119
IP  - 2
DP  - 2023 Feb
TI  - Mycobacterium tuberculosis induces delayed lipid droplet accumulation in 
      dendritic cells depending on bacterial viability and virulence.
PG  - 224-236
LID - 10.1111/mmi.15023 [doi]
AB  - Tuberculosis remains a global health threat with high morbidity. Dendritic cells 
      (DCs) participate in the acute and chronic inflammatory responses to 
      Mycobacterium tuberculosis (Mtb) by directing the adaptive immune response and 
      are present in lung granulomas. In macrophages, the interaction of lipid droplets 
      (LDs) with mycobacteria-containing phagosomes is central to host-pathogen 
      interactions. However, the data available for DCs are still a matter of debate. 
      Here, we reported that bone marrow-derived DCs (BMDCs) were susceptible to Mtb 
      infection and replication at similar rate to macrophages. Unlike macrophages, the 
      analysis of gene expression showed that Mtb infection induced a delayed increase 
      in lipid droplet-related genes and proinflammatory response. Hence, LD 
      accumulation has been observed by high-content imaging in late periods. Infection 
      of BMDCs with killed H37Rv demonstrated that LD accumulation depends on Mtb 
      viability. Moreover, infection with the attenuated strains H37Ra and 
      Mycobacterium bovis-BCG induced only an early transient increase in LDs, whereas 
      virulent Mtb also induced delayed LD accumulation. In addition, infection with 
      the BCG strain with the reintroduced virulence RD1 locus induced higher LD 
      accumulation and bacterial replication when compared to parental BCG. 
      Collectively, our data suggest that delayed LD accumulation in DCs is dependent 
      on mycobacterial viability and virulence.
CI  - (c) 2022 John Wiley & Sons Ltd.
FAU - Costa, Maria Fernanda de Souza
AU  - Costa MFS
AUID- ORCID: 0000-0003-2300-5384
AD  - Instituto de Biologia, Departamento de Imunobiologia, Universidade Federal 
      Fluminense, Niteroi, Brazil.
AD  - Center for Technological Development in Health, Fundacao Oswaldo Cruz, Rio de 
      Janeiro, Brazil.
AD  - Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 
      - CIIL - Center for Infection and Immunity of Lille, Lille, France.
FAU - Pereira-Dutra, Filipe
AU  - Pereira-Dutra F
AUID- ORCID: 0000-0003-1917-2876
AD  - Immunopharmacology Laboratory, Instituto Oswaldo Cruz, Fundacao Oswaldo Cruz, Rio 
      de Janeiro, Brazil.
FAU - Deboosere, Nathalie
AU  - Deboosere N
AD  - Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 
      - CIIL - Center for Infection and Immunity of Lille, Lille, France.
FAU - Jouny, Samuel
AU  - Jouny S
AD  - Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 
      - CIIL - Center for Infection and Immunity of Lille, Lille, France.
FAU - Song, Ok-Ryul
AU  - Song OR
AD  - Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 
      - CIIL - Center for Infection and Immunity of Lille, Lille, France.
FAU - Iack, Guilherme
AU  - Iack G
AD  - Instituto de Biologia, Departamento de Imunobiologia, Universidade Federal 
      Fluminense, Niteroi, Brazil.
AD  - Immunopharmacology Laboratory, Instituto Oswaldo Cruz, Fundacao Oswaldo Cruz, Rio 
      de Janeiro, Brazil.
FAU - Souza, Andreia Lamoglia
AU  - Souza AL
AD  - Fundacao Oswaldo Cruz, Laboratory of Immunology and Immunogenetics in Infectious 
      Diseases at Evandro Chagas National Institute of Infectious Diseases, Rio de 
      Janeiro, Brazil.
FAU - Roma, Eric Henrique
AU  - Roma EH
AD  - Fundacao Oswaldo Cruz, Laboratory of Immunology and Immunogenetics in Infectious 
      Diseases at Evandro Chagas National Institute of Infectious Diseases, Rio de 
      Janeiro, Brazil.
FAU - Delorme, Vincent
AU  - Delorme V
AUID- ORCID: 0000-0001-5235-7069
AD  - Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 
      - CIIL - Center for Infection and Immunity of Lille, Lille, France.
FAU - Bozza, Patricia T
AU  - Bozza PT
AD  - Immunopharmacology Laboratory, Instituto Oswaldo Cruz, Fundacao Oswaldo Cruz, Rio 
      de Janeiro, Brazil.
FAU - Brodin, Priscille
AU  - Brodin P
AD  - Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 
      - CIIL - Center for Infection and Immunity of Lille, Lille, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230116
PL  - England
TA  - Mol Microbiol
JT  - Molecular microbiology
JID - 8712028
RN  - 0 (BCG Vaccine)
SB  - IM
MH  - *Mycobacterium tuberculosis/genetics
MH  - Lipid Droplets
MH  - Virulence
MH  - Microbial Viability
MH  - BCG Vaccine/metabolism
MH  - Dendritic Cells/metabolism/microbiology
OTO - NOTNLM
OT  - RD1
OT  - dendritic cell
OT  - immunometabolism
OT  - lipid droplets
OT  - tuberculosis
EDAT- 2022/12/30 06:00
MHDA- 2023/03/15 06:00
CRDT- 2022/12/29 04:35
PHST- 2022/12/23 00:00 [revised]
PHST- 2021/11/30 00:00 [received]
PHST- 2022/12/25 00:00 [accepted]
PHST- 2022/12/30 06:00 [pubmed]
PHST- 2023/03/15 06:00 [medline]
PHST- 2022/12/29 04:35 [entrez]
AID - 10.1111/mmi.15023 [doi]
PST - ppublish
SO  - Mol Microbiol. 2023 Feb;119(2):224-236. doi: 10.1111/mmi.15023. Epub 2023 Jan 16.