PMID- 36584200 OWN - NLM STAT- MEDLINE DCOM- 20230103 LR - 20230112 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 17 IP - 12 DP - 2022 TI - Exploring the relationship between pancreatic fat and insulin secretion in overweight or obese women without type 2 diabetes mellitus: A preliminary investigation of the TOFI_Asia cohort. PG - e0279085 LID - 10.1371/journal.pone.0279085 [doi] LID - e0279085 AB - OBJECTIVE: While there is an emerging role of pancreatic fat in the aetiology of type 2 diabetes mellitus (T2DM), its impact on the associated decrease in insulin secretion remains controversial. We aimed to determine whether pancreatic fat negatively affects beta-cell function and insulin secretion in women with overweight or obesity but without T2DM. METHODS: 20 women, with normo- or dysglycaemia based on fasting plasma glucose levels, and low (< 4.5%) vs high (>/= 4.5%) magnetic resonance (MR) quantified pancreatic fat, completed a 1-hr intravenous glucose tolerance test (ivGTT) which included two consecutive 30-min square-wave steps of hyperglycaemia generated by using 25% dextrose. Plasma glucose, insulin and C-peptide were measured, and insulin secretion rate (ISR) calculated using regularisation deconvolution method from C-peptide kinetics. Repeated measures linear mixed models, adjusted for ethnicity and baseline analyte concentrations, were used to compare changes during the ivGTT between high and low percentage pancreatic fat (PPF) groups. RESULTS: No ethnic differences in anthropomorphic variables, body composition, visceral adipose tissue (MR-VAT) or PPF were measured and hence data were combined. Nine women (47%) were identified as having high PPF values. PPF was significantly associated with baseline C-peptide (p = 0.04) and ISR (p = 0.04) in all. During the 1-hr ivGTT, plasma glucose (p<0.0001), insulin (p<0.0001) and ISR (p = 0.02) increased significantly from baseline in both high and low PPF groups but did not differ between the two groups at any given time during the test (PPF x time, p > 0.05). Notably, the incremental areas under the curves for both first and second phase ISR were 0.04 units lower in the high than low PPF groups, but this was not significant (p > 0.05). CONCLUSION: In women with overweight or obesity but without T2DM, PPF did not modify beta-cell function as determined by ivGTT-assessed ISR. However, the salient feature in biphasic insulin secretion in those with >/=4.5% PPF may be of clinical importance, particularly in early stages of dysglycaemia may warrant further investigation. CI - Copyright: (c) 2022 Sequeira et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Sequeira, Ivana R AU - Sequeira IR AUID- ORCID: 0000-0001-5414-9925 AD - Human Nutrition Unit, School of Biological Sciences, Faculty of Science, University of Auckland, Auckland, New Zealand. AD - High-Value Nutrition National Science Challenge, Auckland, New Zealand. FAU - Yip, Wilson AU - Yip W AD - Human Nutrition Unit, School of Biological Sciences, Faculty of Science, University of Auckland, Auckland, New Zealand. AD - High-Value Nutrition National Science Challenge, Auckland, New Zealand. FAU - Lu, Louise W AU - Lu LW AD - Human Nutrition Unit, School of Biological Sciences, Faculty of Science, University of Auckland, Auckland, New Zealand. AD - High-Value Nutrition National Science Challenge, Auckland, New Zealand. FAU - Jiang, Yannan AU - Jiang Y AD - Department of Statistics, Faculty of Science, University of Auckland, Auckland, New Zealand. FAU - Murphy, Rinki AU - Murphy R AD - High-Value Nutrition National Science Challenge, Auckland, New Zealand. AD - Department of Medicine, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand. AD - Auckland District Health Board, Auckland, New Zealand. AD - Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, Auckland, New Zealand. FAU - Plank, Lindsay D AU - Plank LD AD - Department of Surgery, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand. FAU - Cooper, Garth J S AU - Cooper GJS AD - Division of Cardiovascular Sciences, Centre for Advanced Discovery and Experimental Therapeutics (CADET), Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom. AD - School of Biological Sciences, Faculty of Science, University of Auckland, Auckland, New Zealand. AD - Division of Medical Sciences, Department of Pharmacology, University of Oxford, Oxford, United Kingdom. FAU - Peters, Carl N AU - Peters CN AD - Department of Medicine, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand. AD - Waitemata District Health Board, Auckland, New Zealand. FAU - Aribsala, Benjamin S AU - Aribsala BS AD - Newcastle Magnetic Resonance Centre, Translational and Clinical Research Institute, Faculty of Medical Science, Newcastle University, Newcastle Upon Tyne, United Kingdom. AD - Department of Computer Science, Faculty of Science, Lagos State University, Lagos, Nigeria. FAU - Hollingsworth, Kieren G AU - Hollingsworth KG AD - Newcastle Magnetic Resonance Centre, Translational and Clinical Research Institute, Faculty of Medical Science, Newcastle University, Newcastle Upon Tyne, United Kingdom. FAU - Poppitt, Sally D AU - Poppitt SD AD - Human Nutrition Unit, School of Biological Sciences, Faculty of Science, University of Auckland, Auckland, New Zealand. AD - High-Value Nutrition National Science Challenge, Auckland, New Zealand. AD - Department of Medicine, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand. AD - Riddet Centre of Research Excellence (CoRE) for Food and Nutrition, Palmerston North, New Zealand. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20221230 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 0 (Blood Glucose) RN - 0 (C-Peptide) RN - 0 (Insulin) SB - IM MH - Humans MH - Female MH - *Diabetes Mellitus, Type 2 MH - Insulin Secretion MH - Blood Glucose MH - Overweight MH - C-Peptide MH - Insulin/metabolism MH - Obesity MH - *Insulin Resistance/physiology PMC - PMC9803309 COIS- The authors have declared that no competing interests exist. EDAT- 2022/12/31 06:00 MHDA- 2023/01/04 06:00 PMCR- 2022/12/30 CRDT- 2022/12/30 14:06 PHST- 2021/11/23 00:00 [received] PHST- 2022/10/02 00:00 [accepted] PHST- 2022/12/30 14:06 [entrez] PHST- 2022/12/31 06:00 [pubmed] PHST- 2023/01/04 06:00 [medline] PHST- 2022/12/30 00:00 [pmc-release] AID - PONE-D-21-36957 [pii] AID - 10.1371/journal.pone.0279085 [doi] PST - epublish SO - PLoS One. 2022 Dec 30;17(12):e0279085. doi: 10.1371/journal.pone.0279085. eCollection 2022.