PMID- 36585543
OWN - NLM
STAT- MEDLINE
DCOM- 20230223
LR  - 20230223
IS  - 1476-3524 (Electronic)
IS  - 1029-8428 (Linking)
VI  - 41
IP  - 1
DP  - 2023 Feb
TI  - CircUBE3B High Expression Participates in Sevoflurane-Induced Human Hippocampal 
      Neuron Injury via Targeting miR-326 and Regulating MYD88 Expression.
PG  - 16-28
LID - 10.1007/s12640-022-00617-0 [doi]
AB  - The clinical application of Sevoflurane (Sevo) brings about non-negligible neuron 
      injury, leading to postoperative cognitive dysfunction (POCD). However, related 
      pathogenesis is complex and not fully established. We aimed to disclose the role 
      of circRNA UBE3B (circUBE3B) in neuron injury induced by Sevo. Cell viability and 
      apoptosis were determined by CCK-8 and flow cytometry experiments. Inflammation 
      production was monitored by ELISA. The expression of circUBE3B, miR-326, and 
      myeloid differentiation factor 88 (MYD88) mRNA was assessed by quantitative 
      real-time PCR (qPCR). Apoptosis-associated markers and MYD88 protein were 
      quantified by western blot. The putative binding site between miR-326 and 
      circUBE3B or MYD88 was verified by a dual-luciferase reporter experiment, and 
      their binding was validated by a pull-down assay. Sevo treatment weakened cell 
      viability and promoted cell apoptosis and inflammatory response. CircUBE3B 
      expression was elevated in Sevo-treated neurons. Sevo-induced neuron injury was 
      alleviated by circUBE3B downregulation but aggravated by circUBE3B 
      overexpression. MiR-326 was targeted by circUBE3B, and miR-326 inhibition 
      recovered neuron injury that was repressed by circUBE3B absence in Sevo-treated 
      neurons. MiR-326 interacted with MYD88. MiR-326 enrichment attenuated 
      Sevo-induced neuron injury, while these effects were reversed by MYD88 
      overexpression. CircUBE3B dysregulation was involved in Sevo-induced human 
      hippocampal neuron injury via targeting the miR-326/MYD88 network.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Qian, Xinye
AU  - Qian X
AD  - Department of Anesthesiology, Fudan University Huashan Hospital, No. 12, Urumqi 
      Road, Jing'An, Shanghai, 200040, China.
FAU - Zheng, Shanshan
AU  - Zheng S
AD  - Department of Anesthesiology, Fudan University Huashan Hospital, No. 12, Urumqi 
      Road, Jing'An, Shanghai, 200040, China.
FAU - Yu, Yingfang
AU  - Yu Y
AD  - Department of Anesthesiology, Fudan University Huashan Hospital, No. 12, Urumqi 
      Road, Jing'An, Shanghai, 200040, China. xzzqpcc@163.com.
LA  - eng
PT  - Journal Article
DEP - 20221231
PL  - United States
TA  - Neurotox Res
JT  - Neurotoxicity research
JID - 100929017
RN  - 38LVP0K73A (Sevoflurane)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (MicroRNAs)
RN  - EC 2.3.2.26 (UBE3B protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - 0 (MIRN326 microRNA, human)
SB  - IM
MH  - Humans
MH  - Sevoflurane/toxicity
MH  - *Myeloid Differentiation Factor 88/genetics/metabolism/pharmacology
MH  - *MicroRNAs/metabolism
MH  - Hippocampus
MH  - Neurons
MH  - Apoptosis
MH  - Ubiquitin-Protein Ligases/metabolism
OTO - NOTNLM
OT  - MYD88
OT  - Neuron
OT  - Sevoflurane
OT  - circUBE3B
OT  - miR-326
EDAT- 2022/12/31 06:00
MHDA- 2023/02/25 06:00
CRDT- 2022/12/30 23:36
PHST- 2022/06/24 00:00 [received]
PHST- 2022/12/05 00:00 [accepted]
PHST- 2022/09/19 00:00 [revised]
PHST- 2022/12/31 06:00 [pubmed]
PHST- 2023/02/25 06:00 [medline]
PHST- 2022/12/30 23:36 [entrez]
AID - 10.1007/s12640-022-00617-0 [pii]
AID - 10.1007/s12640-022-00617-0 [doi]
PST - ppublish
SO  - Neurotox Res. 2023 Feb;41(1):16-28. doi: 10.1007/s12640-022-00617-0. Epub 2022 
      Dec 31.