PMID- 36585647
OWN - NLM
STAT- MEDLINE
DCOM- 20230103
LR  - 20230103
IS  - 2662-7671 (Electronic)
IS  - 2662-7671 (Linking)
VI  - 22
IP  - 1
DP  - 2022 Dec 30
TI  - The ethanolic extract of Curcuma longa grown in Korea exhibits 
      anti-neuroinflammatory effects by activating of nuclear transcription factor 
      erythroid-2-related factor 2/heme oxygenase-1 signaling pathway.
PG  - 343
LID - 10.1186/s12906-022-03825-5 [doi]
LID - 343
AB  - BACKGROUND: Curcuma longa has been used as spices, food preservative, coloring 
      material, and traditional medicine. This plant also has long been used for a 
      variety of diseases including dyslipidemia, stomach disorders, arthritis, and 
      hepatic diseases. The aim of the present investigation was to examine the 
      anti-neuroinflammatory effects of the 50% ethanolic extract of C. longa in 
      lipopolysaccharide (LPS)-induced BV2 microglial cells. METHODS: Griess reaction 
      was employed to measure the production of nitric oxide (NO), and the levels of 
      prostaglandin E2 (PGE(2)) and pro-inflammatory cytokines such as interleukin 
      1-beta (IL-1beta), IL-6 and tumor necrosis factor-alpha (TNF-alpha) were determined by using 
      profit ELISA kits. Western blotting was used to determine the expression of 
      inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear factor 
      kappa B (NF-kappaB), mitogen activated protein kinases (MAPKs), heme oxygenase-1 
      (HO-1) and nuclear factor erythroid-2-related factor 2 (Nrf2). RESULTS: 
      Pre-treatment with CLE inhibited the overproduction and overexpression of 
      pro-inflammatory mediators including NO, PGE(2), iNOS, COX-2, and 
      pro-inflammatory cytokines such as IL-1beta, IL-6 and TNF-alpha in LPS-induced BV2 
      cells. In addition, CLE suppressed the activation of the NF-kappaB and three MAPK 
      signaling pathways. Treatment with CLE induced HO-1 protein expression by 
      activating Nrf2 pathway, and inhibiting the HO-1 expression reversed the 
      anti-inflammatory effect of CLE. CONCLUSION: CLE showed anti-neuroinflammatory 
      effects against LPS-induced microglial cells activation through the inhibition of 
      production and expression of pro-inflammatory mediators by negative regulation of 
      the NF-kappaB and MAPK signaling pathways. These anti-neuroinflammatory effects of 
      CLE were mediated by HO-1/Nrf2 signaling pathway. Taken together, the present 
      study suggests a potent effect of CLE to prevent neuroinflammatory diseases. It 
      is necessary to perform additional efficacy evaluation through in vivo 
      experiments.
CI  - (c) 2022. The Author(s).
FAU - Kim, Kwan-Woo
AU  - Kim KW
AD  - Department of Herbal Crop Research, National Institute of Horticultural and 
      Herbal Sciences, Rural Development Administration, 27709, Eumseong, Republic of 
      Korea.
FAU - Lee, Young-Seob
AU  - Lee YS
AD  - Department of Herbal Crop Research, National Institute of Horticultural and 
      Herbal Sciences, Rural Development Administration, 27709, Eumseong, Republic of 
      Korea. youngseoblee@korea.kr.
FAU - Yoon, Dahye
AU  - Yoon D
AD  - Department of Herbal Crop Research, National Institute of Horticultural and 
      Herbal Sciences, Rural Development Administration, 27709, Eumseong, Republic of 
      Korea.
FAU - Kim, Geum-Soog
AU  - Kim GS
AD  - Department of Herbal Crop Research, National Institute of Horticultural and 
      Herbal Sciences, Rural Development Administration, 27709, Eumseong, Republic of 
      Korea.
FAU - Lee, Dae Young
AU  - Lee DY
AD  - Department of Herbal Crop Research, National Institute of Horticultural and 
      Herbal Sciences, Rural Development Administration, 27709, Eumseong, Republic of 
      Korea. dylee0809@gmail.com.
LA  - eng
GR  - PJ01497501/Cooperative Research Program for Agricultural Science & Technology 
      Development/
GR  - PJ01497501/Cooperative Research Program for Agricultural Science & Technology 
      Development/
GR  - PJ01497501/Cooperative Research Program for Agricultural Science & Technology 
      Development/
GR  - PJ01497501/Cooperative Research Program for Agricultural Science & Technology 
      Development/
GR  - PJ01497501/Cooperative Research Program for Agricultural Science & Technology 
      Development/
PT  - Journal Article
DEP - 20221230
PL  - England
TA  - BMC Complement Med Ther
JT  - BMC complementary medicine and therapies
JID - 101761232
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Interleukin-6)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-E2-Related Factor 2)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
SB  - IM
MH  - *NF-kappa B/metabolism
MH  - *Tumor Necrosis Factor-alpha/metabolism
MH  - Interleukin-6/metabolism
MH  - Neuroinflammatory Diseases
MH  - Heme Oxygenase-1/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Curcuma
MH  - NF-E2-Related Factor 2/metabolism
MH  - Cyclooxygenase 2/metabolism
MH  - Cell Line
MH  - Signal Transduction
MH  - Cytokines/metabolism
MH  - Inflammation Mediators
MH  - Republic of Korea
PMC - PMC9804997
OTO - NOTNLM
OT  - Anti-neuroinflammation
OT  - BV2 microglial cells
OT  - Curcuma longa
OT  - Heme oxigenase-1
OT  - Mitogen-activated protein kinases
OT  - Nuclear factor kappa B
COIS- The authors declare that they have no conflict of interest.
EDAT- 2022/12/31 06:00
MHDA- 2023/01/04 06:00
PMCR- 2022/12/30
CRDT- 2022/12/30 23:46
PHST- 2022/09/07 00:00 [received]
PHST- 2022/12/20 00:00 [accepted]
PHST- 2022/12/30 23:46 [entrez]
PHST- 2022/12/31 06:00 [pubmed]
PHST- 2023/01/04 06:00 [medline]
PHST- 2022/12/30 00:00 [pmc-release]
AID - 10.1186/s12906-022-03825-5 [pii]
AID - 3825 [pii]
AID - 10.1186/s12906-022-03825-5 [doi]
PST - epublish
SO  - BMC Complement Med Ther. 2022 Dec 30;22(1):343. doi: 10.1186/s12906-022-03825-5.