PMID- 36585800
OWN - NLM
STAT- MEDLINE
DCOM- 20230503
LR  - 20230516
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 111
IP  - 7
DP  - 2023 Jul
TI  - Design and characterization of lipid nanocarriers for oral delivery of 
      immunotherapeutic peptides.
PG  - 938-949
LID - 10.1002/jbm.a.37477 [doi]
AB  - The use of therapeutic proteins and peptides is of great interest for the 
      treatment of many diseases, and advances in nanotechnology offer a path toward 
      their stable delivery via preferred routes of administration. In this study, we 
      sought to design and formulate a nanostructured lipid carrier (NLC) containing a 
      nominal antigen (insulin peptide) for oral delivery. We utilized the design of 
      experiments (DOE) statistical method to determine the dependencies of formulation 
      variables on physicochemical particle characteristics including particle size, 
      polydispersity (PDI), melting point, and latent heat of melting. The particles 
      were determined to be non-toxic in vitro, readily taken up by primary immune 
      cells, and found to accumulate in regional lymph nodes following oral 
      administration. We believe that this platform technology could be broadly useful 
      for the treatment of autoimmune diseases by supporting the development of oral 
      delivery-based antigen specific immunotherapies.
CI  - (c) 2022 JHU/APL. Journal of Biomedical Materials Research Part A published by 
      Wiley Periodicals LLC.
FAU - Calderon-Colon, Xiomara
AU  - Calderon-Colon X
AD  - Johns Hopkins Applied Physics Laboratory, Laurel, Maryland, USA.
FAU - Zhang, Yichuan
AU  - Zhang Y
AD  - Department of Plastic & Reconstructive Surgery, Johns Hopkins University School 
      of Medicine, Baltimore, Maryland, USA.
FAU - Tiburzi, Olivia
AU  - Tiburzi O
AD  - Johns Hopkins Applied Physics Laboratory, Laurel, Maryland, USA.
FAU - Wang, Jialu
AU  - Wang J
AD  - Department of Plastic & Reconstructive Surgery, Johns Hopkins University School 
      of Medicine, Baltimore, Maryland, USA.
FAU - Hou, Shenda
AU  - Hou S
AD  - Department of Plastic & Reconstructive Surgery, Johns Hopkins University School 
      of Medicine, Baltimore, Maryland, USA.
FAU - Raimondi, Giorgio
AU  - Raimondi G
AD  - Department of Plastic & Reconstructive Surgery, Johns Hopkins University School 
      of Medicine, Baltimore, Maryland, USA.
FAU - Patrone, Julia
AU  - Patrone J
AUID- ORCID: 0000-0003-3477-9502
AD  - Johns Hopkins Applied Physics Laboratory, Laurel, Maryland, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221230
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Drug Carriers)
RN  - 0 (Lipids)
SB  - IM
MH  - *Drug Carriers/chemistry
MH  - Lipids/chemistry
MH  - *Nanostructures/chemistry
MH  - Nanotechnology
MH  - Administration, Oral
MH  - Particle Size
OTO - NOTNLM
OT  - insulin peptide
OT  - lipid nanoparticle
OT  - lymphoid tissues
OT  - oral delivery
EDAT- 2023/01/01 06:00
MHDA- 2023/05/03 06:42
CRDT- 2022/12/31 02:02
PHST- 2022/10/07 00:00 [revised]
PHST- 2022/04/27 00:00 [received]
PHST- 2022/11/30 00:00 [accepted]
PHST- 2023/05/03 06:42 [medline]
PHST- 2023/01/01 06:00 [pubmed]
PHST- 2022/12/31 02:02 [entrez]
AID - 10.1002/jbm.a.37477 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2023 Jul;111(7):938-949. doi: 10.1002/jbm.a.37477. Epub 
      2022 Dec 30.