PMID- 36592642
OWN - NLM
STAT- MEDLINE
DCOM- 20230203
LR  - 20230522
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 325
DP  - 2023 Feb
TI  - Association of HLADQ-B1 polymorphisms in three generations of chronic hepatitis B 
      patients.
PG  - 199036
LID - S0168-1702(22)00365-3 [pii]
LID - 10.1016/j.virusres.2022.199036 [doi]
LID - 199036
AB  - The presence of polymorphisms in the human leukocyte antigen (HLA)-DQB1 gene, 
      along with its expression, has been demonstrated to be correlated with 
      spontaneous clearance and susceptibility to HBV infection. The present study 
      aimed to evaluate the possible role of genetic polymorphisms in HLA-DQB1 in three 
      generations of patients with chronic hepatitis B (CHB). Based on the inclusion 
      criteria, 90 CHB patients, 18 individuals recovered from HBV infection, and 40 
      healthy subjects were chosen. The DNA contents of the whole blood samples were 
      extracted in order to perform HLA-DQB1 typing by the PCR technique. Besides whole 
      blood samples, sera were applied to measure liver function tests (LFTs), as well 
      as the titers of anti-HDV and anti-HCV. Also, in all CHB patients were measured 
      liver stiffness (LSM) by Fibro Scan. The results of HLA-DQB1 polymorphisms 
      (rs2856718 and rs7453920) demonstrated that the majority of polymorphisms in CHB 
      patients were HLA-DQB1*03, HLA-DQB1*05, HLA-DQB1*04:01 and HLA-DQB1*03:01 that 
      associated with HBV persistence and chronicity. Among the patients who showed 
      these polymorphisms, the mean+/-SD, LSM was 4+/-1.57 KPa and most of them, F grade 
      was reported as F2, which was a sign of disease progression towards chronicity. 
      HLA polymorphisms imputation revealed that HLA-DQB1*06:04 (3.4%, P-Value= 0.2) 
      was detected only in healthy subjects as protective polymorphism, while the 
      allele HLA-DQB1*03:03 was reported in both healthy subjects (P-Value= 0.06) and 
      recovered patients (P-Value= 0.1) as suppressor of CHB formation. The allele 
      HLA-DQB1*05:02 was found in both healthy subjects (3.4%) and CHB patients (4.5%) 
      which was associated with risk to liver cirrhosis (P-Value= 0, OR: 0.002 0.95CI: 
      0.000-0.15). HLA polymorphism analysis indicated that 17.39% of patients who were 
      seropositive for anti-HCV carried the HLA-DQB1*03:01. HBV resistance or infection 
      risk could be assessed by DBQ1 typing. The existence of polymorphisms in HLA gene 
      could influence the clearance (HLA-DQB1*03:03) or susceptibility and persistence 
      of infection (HLA-DQB1*03, HLA-DQB1*05, HLA-DQB1*04:01 and HLA-DQB1*03:01). These 
      results have the potential to improve personalized therapy and prognosis for HBV 
      infection.
CI  - Copyright (c) 2022. Published by Elsevier B.V.
FAU - Naderi, Malihe
AU  - Naderi M
AD  - Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences 
      and Biotechnology, Shahid Beheshti University, Tehran, Iran; Department of 
      Microbiology, Faculty of Medicine, Golestan University of Medical Sciences, 
      Gorgan, Iran.
FAU - Hosseini, Seyed Masoud
AU  - Hosseini SM
AD  - Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences 
      and Biotechnology, Shahid Beheshti University, Tehran, Iran. Electronic address: 
      ma_Hosseini@sbu.ac.ir.
FAU - Behnampour, Naser
AU  - Behnampour N
AD  - Biostatistics and Epidemiology Department, Faculty of Health, Health Management 
      and Social Development Research Center, Golestan University of Medical Sciences, 
      Golestan, Iran.
FAU - Shahramian, Iraj
AU  - Shahramian I
AD  - Department of Pediatrics, Faculty of Medicine, Shiraz University of Medical 
      Sciences, Shiraz, Iran.
FAU - Moradi, Abdolvahab
AU  - Moradi A
AD  - Department of Microbiology, Faculty of Medicine, Golestan University of Medical 
      Sciences, Gorgan, Iran. Electronic address: abmoradi@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20221230
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
SB  - IM
MH  - Humans
MH  - *Hepatitis B, Chronic/genetics
MH  - Genetic Predisposition to Disease
MH  - Polymorphism, Genetic
MH  - Alleles
MH  - Liver Cirrhosis/genetics
MH  - Hepatitis B virus/genetics
MH  - *Hepatitis B/genetics
PMC - PMC10194363
OTO - NOTNLM
OT  - HBV
OT  - HLA-DQB1
OT  - Polymorphisms, anti-HCV, anti-HDV
COIS- Declaration of interests The authors declare that they have no known competing 
      financial interests or personal relationships that could have appeared to 
      influence the work reported in this paper.
EDAT- 2023/01/03 06:00
MHDA- 2023/02/04 06:00
PMCR- 2022/12/30
CRDT- 2023/01/02 19:11
PHST- 2022/12/07 00:00 [received]
PHST- 2022/12/28 00:00 [revised]
PHST- 2022/12/29 00:00 [accepted]
PHST- 2023/01/03 06:00 [pubmed]
PHST- 2023/02/04 06:00 [medline]
PHST- 2023/01/02 19:11 [entrez]
PHST- 2022/12/30 00:00 [pmc-release]
AID - S0168-1702(22)00365-3 [pii]
AID - 199036 [pii]
AID - 10.1016/j.virusres.2022.199036 [doi]
PST - ppublish
SO  - Virus Res. 2023 Feb;325:199036. doi: 10.1016/j.virusres.2022.199036. Epub 2022 
      Dec 30.