PMID- 36598094
OWN - NLM
STAT- MEDLINE
DCOM- 20230119
LR  - 20230119
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 71
IP  - 2
DP  - 2023 Jan 18
TI  - UHPLC-Q-Orbitrap-Based Integrated Lipidomics and Proteomics Reveal 
      Propane-1,2-diol Exposure Accelerating Degradation of Lipids via the Allosteric 
      Effect and Reducing the Nutritional Value of Milk.
PG  - 1178-1189
LID - 10.1021/acs.jafc.2c07059 [doi]
AB  - The scandal of detecting the flavoring solvent propane-1,2-diol (PD) in milk has 
      brought a crisis to the trust of consumers in the dairy industry, while its 
      deposition and transformation are still indistinct. Pseudo-targeted lipidomics 
      revealed that PD accelerated the degradation of glycerolipid (33,638.3 +/- 28.9 to 
      104,54.2 +/- 28.4 mg kg(-1)), phosphoglyceride (467.4 +/- 8.2 to 56.6 +/- 4.2 mg 
      kg(-1)), and sphingolipids (11.4 +/- 0.3 to 0.7 +/- 0.2 mg kg(-1)), which extremely 
      decreased the milk quality. Recoveries and relative standard deviations (RSDs) of 
      the established method were 85.0-109.9 and 0.1-14.9%, respectively, indicating 
      that the approach was credible. Protein-lipid interactions demonstrated that 10 
      proteins originating from fat globules were upregulated significantly and the 
      activities of 7 enzymes related to lipid degradation were improved. 
      Diacylglycerol cholinephosphotransferase was the only enzyme with decreased 
      activity, and the molecular docking results indicated that PD adjusted its 
      activity through regulating the conformation of the active center and weakening 
      the hydrogen bond force between the enzyme and substrate. This study firstly 
      revealed the mechanism of deposition and transformation of PD in milk, which 
      contributed to the knowledge on the milk quality control and provided key 
      indicators to evaluate the adverse risks of PD in dairy products.
FAU - Fan, Zibian
AU  - Fan Z
AD  - School of Food and Biological Engineering, Shaanxi University of Science & 
      Technology, Xi'an710021, China.
FAU - Jia, Wei
AU  - Jia W
AUID- ORCID: 0000-0001-5573-9361
AD  - School of Food and Biological Engineering, Shaanxi University of Science & 
      Technology, Xi'an710021, China.
AD  - Shaanxi Research Institute of Agricultural Products Processing Technology, 
      Xi'an710021, China.
FAU - Du, An
AU  - Du A
AD  - School of Food and Biological Engineering, Shaanxi University of Science & 
      Technology, Xi'an710021, China.
LA  - eng
PT  - Journal Article
DEP - 20230104
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - T75W9911L6 (Propane)
RN  - 0 (Phospholipids)
SB  - IM
MH  - Animals
MH  - *Milk/chemistry
MH  - *Propane/analysis/metabolism
MH  - Lipidomics/methods
MH  - Proteomics/methods
MH  - Chromatography, High Pressure Liquid/methods
MH  - Molecular Docking Simulation
MH  - Phospholipids/metabolism
MH  - Nutritive Value
OTO - NOTNLM
OT  - UHPLC-Q-orbitrap
OT  - deep single-shot profiling strategy
OT  - pasteurized milk
OT  - propane-1,2-diol
OT  - proteomics
OT  - pseudo-targeted lipidomics
EDAT- 2023/01/05 06:00
MHDA- 2023/01/20 06:00
CRDT- 2023/01/04 07:22
PHST- 2023/01/05 06:00 [pubmed]
PHST- 2023/01/20 06:00 [medline]
PHST- 2023/01/04 07:22 [entrez]
AID - 10.1021/acs.jafc.2c07059 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2023 Jan 18;71(2):1178-1189. doi: 10.1021/acs.jafc.2c07059. 
      Epub 2023 Jan 4.