PMID- 36598130
OWN - NLM
STAT- MEDLINE
DCOM- 20240226
LR  - 20240226
IS  - 1699-5848 (Electronic)
IS  - 0213-3911 (Linking)
VI  - 39
IP  - 3
DP  - 2024 Mar
TI  - Catalpol protects mouse ATDC5 chondrocytes against interleukin-1beta-induced 
      catabolism.
PG  - 333-344
LID - 10.14670/HH-18-575 [doi]
AB  - Catalpol is a natural product with promising anti-inflammatory effects, however, 
      its effects on chondrocytes and osteoarthritis (OA) have not been well 
      investigated. OA is a painful and debilitating joint disease that affects people 
      worldwide. Traditional Chinese Medicine has been sought to treat OA, including 
      the Rehmannia extract, Catalpol. Here, we examined the effects of Catalpol, a 
      plant derivative used in traditional Chinese medicine, on ATDC5 chondrocytes 
      originating from mouse teratocarcinoma cells stimulated with interleukin-1beta 
      (IL-1beta) to mimic the OA cellular environment. Catalpol significantly reduced 
      matrix metalloproteinase-1, -3, -13 (MMP-1, -3, -13), a disintegrin and 
      metalloproteinase with thrombospondin motifs -4, -5 (ADAMTS-4, -5) against IL-1beta, 
      demonstrating a likely anti-cartilage degradation activity. We also found that 
      Catalpol exerted a significant anti-oxidative stress effect by downregulating the 
      production of inducible nitric oxide synthase (iNOS), nitric oxide (NO), reactive 
      oxygen species (ROS), and malondialdehyde (MDA). Catalpol treatment significantly 
      reduced the levels of several key inflammatory factors, including Prostaglandin 
      E(2) (PGE(2)), cyclooxygenase-2 (COX-2), interleukin-8 (IL-8), and monocyte 
      chemoattractant protein-1 (MCP-1). We further demonstrate that the effects of 
      Catalpol were mediated by the nuclear factor -kappaB (NF-kappaB) pathway via 
      downregulation of the phosphorylation of inhibitor of nuclear factor kappaB-alpha (IkappaBalpha). 
      This was confirmed by measuring p38 and p65 protein levels as well as the 
      luciferase activity of NF-kappaB. Altogether, we demonstrate the potential of 
      Catalpol as a novel treatment agent against cartilage matrix degradation, 
      oxidative stress, and inflammation in OA.
CI  - (c)The Author(s) 2024. Open Access. This article is licensed under a Creative 
      Commons CC-BY International License.
FAU - Cai, Chengkui
AU  - Cai C
AD  - Department of Orthopedics, The Second Affiliated Hospital of Shaanxi University 
      of Chinese Medicine, Xianyang, Shaanxi, PR China.
FAU - Sun, Pengcheng
AU  - Sun P
AD  - Department of Orthopedics, The Second Affiliated Hospital of Shaanxi University 
      of Chinese Medicine, Xianyang, Shaanxi, PR China.
FAU - Chen, Zhihui
AU  - Chen Z
AD  - Department of Orthopedics, The Second Affiliated Hospital of Shaanxi University 
      of Chinese Medicine, Xianyang, Shaanxi, PR China.
FAU - Sun, Chao
AU  - Sun C
AD  - Department of Orthopedics, The Second Affiliated Hospital of Shaanxi University 
      of Chinese Medicine, Xianyang, Shaanxi, PR China.
FAU - Tian, Liying
AU  - Tian L
AD  - Department of Anesthesiology, The Second Affiliated Hospital of Air Force Medical 
      University, Xi' an, Shaanxi, PR China. tianly0323@yeah.net.
LA  - eng
GR  - SAHSGMU-2018U03/Second Affiliated Hospital of Air Force Medical University/
PT  - Journal Article
DEP - 20221215
PL  - Spain
TA  - Histol Histopathol
JT  - Histology and histopathology
JID - 8609357
RN  - 0 (NF-kappa B)
RN  - 0 (Interleukin-1beta)
RN  - 2415-24-9 (catalpol)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Iridoid Glucosides)
SB  - IM
MH  - Humans
MH  - Mice
MH  - Animals
MH  - *Chondrocytes/metabolism
MH  - Inflammation/pathology
MH  - NF-kappa B/metabolism
MH  - Interleukin-1beta/metabolism
MH  - Anti-Inflammatory Agents/therapeutic use
MH  - *Osteoarthritis/metabolism
MH  - *Iridoid Glucosides
EDAT- 2023/01/05 06:00
MHDA- 2024/02/26 06:43
CRDT- 2023/01/04 07:53
PHST- 2024/02/26 06:43 [medline]
PHST- 2023/01/05 06:00 [pubmed]
PHST- 2023/01/04 07:53 [entrez]
AID - HH-18-575 [pii]
AID - 10.14670/HH-18-575 [doi]
PST - ppublish
SO  - Histol Histopathol. 2024 Mar;39(3):333-344. doi: 10.14670/HH-18-575. Epub 2022 
      Dec 15.