PMID- 36599452
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231231
IS  - 1305-7456 (Print)
IS  - 1305-7464 (Electronic)
VI  - 17
IP  - 4
DP  - 2023 Oct
TI  - Osteoinductive and Osteogenic Capacity of Freeze-Dried Bovine Bone Compared to 
      Deproteinized Bovine Bone Mineral Scaffold in Human Umbilical Cord Mesenchymal 
      Stem Cell Culture: An In Vitro Study.
PG  - 1106-1113
LID - 10.1055/s-0042-1758786 [doi]
AB  - OBJECTIVE:  Freeze-dried bovine bone scaffold (FDBB) or decellularized FDBB 
      (dc-FDBB) was developed as an ideal scaffold with osteoinductive properties. This 
      research aims to compare the osteoinductive properties marked by the expression 
      of runt-related transcription factor-2 (RUNX2) and Osterix (OSX) and the 
      osteogenic capacity of these scaffolds imbued with human umbilical cord 
      mesenchymal stem cells (hUCMSCs). MATERIALS AND METHODS:  This study was 
      performed in five experimental groups: a negative control group (C-) of hUCMSCs 
      with a normal growth medium, a positive control group (C + ) of hUCMSCs with an 
      osteogenic medium, experimental group 1 (E1) with an FDBB conditioned medium 
      (CM), and experimental group 2 (E2) with a dc-FDBB-CM, and a third experimental 
      group (E3) consisting of a DBBM-CM. Alizarin red staining was performed to 
      qualitatively assess osteoinductive capacity. RUNX2 and OSX expression was 
      quantified using real-time quantification polymerase chain reaction with two 
      replications on day six (D6) and day 12 (D12) as fold changes. RESULTS:  This 
      experiment revealed that hUCMSCs were positively expressed by CD73, CD90, and 
      CD105 but were not expressed by CD34. Alizarin red staining showed that E1 had 
      the most calcium deposition on D6 and D12, followed by E3 and then E2 The RUNX2 
      and OSX expression was higher in E1 but this difference was not significant. The 
      OSX expression in E1,E2,E3 was lower on D12 and C+ of OSX had the highest 
      expression. There was a significant difference of fold change measured between 
      all groups (p < 0.05), and there was no significant difference between any of the 
      groups treated with OSX and RUNX2 on D6 and D12. CONCLUSION:  FDBB osteoinduction 
      and osteogenic capacity were higher when compared with DBBM and dc-FDBB.
CI  - The Author(s). This is an open access article published by Thieme under the terms 
      of the Creative Commons Attribution License, permitting unrestricted use, 
      distribution, and reproduction so long as the original work is properly cited. 
      (https://creativecommons.org/licenses/by/4.0/).
FAU - Nugraha, Andreas Pratama
AU  - Nugraha AP
AD  - Magister Program of Clinical Medicine, Faculty of Medicine, Universitas 
      Airlangga, Surabaya, Indonesia.
AD  - Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, 
      Universitas Airlangga, Surabaya, Indonesia.
FAU - Kamadjaja, David B
AU  - Kamadjaja DB
AD  - Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, 
      Universitas Airlangga, Surabaya, Indonesia.
FAU - Sumarta, Ni Putu Mira
AU  - Sumarta NPM
AD  - Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, 
      Universitas Airlangga, Surabaya, Indonesia.
FAU - Rizqiawan, Andra
AU  - Rizqiawan A
AD  - Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, 
      Universitas Airlangga, Surabaya, Indonesia.
FAU - Pramono, Coen
AU  - Pramono C
AD  - Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, 
      Universitas Airlangga, Surabaya, Indonesia.
FAU - Yuliati, Anita
AU  - Yuliati A
AD  - Departement of Dental Material, Faculty of Dental medicine Universitas Airlangga, 
      Surabaya, Indonesia.
FAU - Hendrianto, Eryk
AU  - Hendrianto E
AD  - Stem Cell Research and Developmental Center, Universitas Airlangga, Surabaya, 
      Indonesia.
FAU - Rahman, Mohammad Zeshaan
AU  - Rahman MZ
AD  - Department of Oral and Maxillofacial Surgery, Pioneer Dental College and 
      Hospital, Dhaka, Bangladesh.
LA  - eng
PT  - Journal Article
DEP - 20230104
PL  - Germany
TA  - Eur J Dent
JT  - European journal of dentistry
JID - 101303672
PMC - PMC10756842
COIS- None declared.
EDAT- 2023/01/05 06:00
MHDA- 2023/01/05 06:01
PMCR- 2023/01/01
CRDT- 2023/01/04 20:32
PHST- 2023/01/05 06:01 [medline]
PHST- 2023/01/05 06:00 [pubmed]
PHST- 2023/01/04 20:32 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - EJD-22-7-2232 [pii]
AID - 10.1055/s-0042-1758786 [doi]
PST - ppublish
SO  - Eur J Dent. 2023 Oct;17(4):1106-1113. doi: 10.1055/s-0042-1758786. Epub 2023 Jan 
      4.