PMID- 36600931
OWN - NLM
STAT- MEDLINE
DCOM- 20230106
LR  - 20230111
IS  - 2210-7185 (Electronic)
IS  - 2210-7177 (Print)
IS  - 2210-7177 (Linking)
VI  - 2022
DP  - 2022
TI  - Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through 
      Nogo/NgR Pathway.
PG  - 4588999
LID - 10.1155/2022/4588999 [doi]
LID - 4588999
AB  - The effect of Shenfu injection on brain injury after cardiac arrest (CA) and 
      cardiopulmonary resuscitation (CPR) along with the underlying mechanism of axonal 
      regeneration was explored. CA/CPR model in rats was established for subsequent 
      experiments. A total of 160 rats were randomly divided into sham group, model 
      group, conventional western medicine (CWM) group, Shenfu group, and antagonist 
      group (n = 32 per group). After 3 hours, 24 hours, 3 days, and 7 days of drug 
      administration, the modified Neurological Severity Score tests were performed. 
      The ultrastructure of the brain and hippocampus was observed by electron 
      microscopy. Real-time quantitative polymerase chain reaction (PCR), western 
      blotting, and immunohistochemistry were used to detect Nogo receptor (NgR) 
      expression in the hippocampus and cerebral cortex, and Nogo-NgR expression in 
      CA/CPR model. Neurological deficits in the model group were severe at 3 hours, 24 
      hours, 3 days, and 7 days after the recovery of natural circulation, whereas the 
      neurological deficits in CWM, antagonist, and Shenfu group were relatively mild. 
      The ultrastructure of neuronal cells in Shenfu group had relatively complete cell 
      membranes and more vesicles than those in the model group. The results of PCR and 
      western blotting showed lower messenger ribonucleic acid and protein expression 
      of NgR in Shenfu group than the model group and CWM group. Immunohistochemical 
      examination indicated a reduction of Nogo-NgR expression in Shenfu group and 
      antagonist group. Our results suggested that Shenfu injection reduced brain 
      injury by attenuating Nogo-NgR signaling pathway and promoting axonal 
      regeneration.
CI  - Copyright (c) 2022 Haixia Deng et al.
FAU - Deng, Haixia
AU  - Deng H
AUID- ORCID: 0000-0002-3403-5186
AD  - Department of Surgical Intensive Care Unit, The First Affiliated Hospital of 
      Guangxi Medical University, Nanning 530021, China.
AD  - Department of Emergency, The First Affiliated Hospital of Guangxi University of 
      Chinese Medicine, Nanning 530022, China.
FAU - Tang, Zhanhong
AU  - Tang Z
AUID- ORCID: 0000-0001-6698-4098
AD  - Department of Surgical Intensive Care Unit, The First Affiliated Hospital of 
      Guangxi Medical University, Nanning 530021, China.
FAU - Tuo, Peng
AU  - Tuo P
AUID- ORCID: 0000-0002-8132-7288
AD  - Department of Emergency, The First Affiliated Hospital of Guangxi University of 
      Chinese Medicine, Nanning 530022, China.
FAU - Wu, Ruihua
AU  - Wu R
AUID- ORCID: 0000-0002-0666-3169
AD  - Guangxi University of Chinese Medicine, Nanning 530200, China.
FAU - Jia, Si
AU  - Jia S
AUID- ORCID: 0000-0001-9038-8317
AD  - Guangxi University of Chinese Medicine, Nanning 530200, China.
FAU - Zhao, Xuan
AU  - Zhao X
AUID- ORCID: 0000-0001-9810-9896
AD  - Department of Emergency, The First Affiliated Hospital of Guangxi University of 
      Chinese Medicine, Nanning 530022, China.
FAU - Huang, Deqing
AU  - Huang D
AUID- ORCID: 0000-0003-2384-2801
AD  - Department of Emergency, The First Affiliated Hospital of Guangxi University of 
      Chinese Medicine, Nanning 530022, China.
FAU - Gao, Yuguang
AU  - Gao Y
AUID- ORCID: 0000-0003-1704-6235
AD  - Department of Emergency, The First Affiliated Hospital of Guangxi University of 
      Chinese Medicine, Nanning 530022, China.
FAU - Lan, Zhou
AU  - Lan Z
AUID- ORCID: 0000-0001-5197-0195
AD  - Department of Emergency, The First Affiliated Hospital of Guangxi University of 
      Chinese Medicine, Nanning 530022, China.
LA  - eng
PT  - Journal Article
DEP - 20221226
PL  - United States
TA  - Anal Cell Pathol (Amst)
JT  - Analytical cellular pathology (Amsterdam)
JID - 101541993
RN  - 0 (Nogo Receptors)
RN  - 0 (Myelin Proteins)
RN  - 0 (Shen-Fu)
RN  - 0 (Nogo Proteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Nogo Receptor 1)
RN  - 0 (GPI-Linked Proteins)
SB  - IM
MH  - Rats
MH  - Animals
MH  - Nogo Receptors
MH  - Rats, Sprague-Dawley
MH  - Myelin Proteins/analysis/metabolism
MH  - Nogo Proteins
MH  - Receptors, Cell Surface/metabolism
MH  - Nogo Receptor 1
MH  - GPI-Linked Proteins/metabolism
MH  - *Brain Injuries/drug therapy/metabolism
MH  - *Heart Arrest/complications/drug therapy
PMC - PMC9807299
COIS- The author(s) declare(s) that they have no conflicts of interest.
EDAT- 2023/01/06 06:00
MHDA- 2023/01/07 06:00
PMCR- 2022/12/26
CRDT- 2023/01/05 02:18
PHST- 2021/11/07 00:00 [received]
PHST- 2022/05/23 00:00 [revised]
PHST- 2022/11/19 00:00 [accepted]
PHST- 2023/01/05 02:18 [entrez]
PHST- 2023/01/06 06:00 [pubmed]
PHST- 2023/01/07 06:00 [medline]
PHST- 2022/12/26 00:00 [pmc-release]
AID - 10.1155/2022/4588999 [doi]
PST - epublish
SO  - Anal Cell Pathol (Amst). 2022 Dec 26;2022:4588999. doi: 10.1155/2022/4588999. 
      eCollection 2022.