PMID- 36605203
OWN - NLM
STAT- MEDLINE
DCOM- 20230109
LR  - 20231213
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 13
DP  - 2022
TI  - Complement component 3 protects human bronchial epithelial cells from cigarette 
      smoke-induced oxidative stress and prevents incessant apoptosis.
PG  - 1035930
LID - 10.3389/fimmu.2022.1035930 [doi]
LID - 1035930
AB  - The complement component 3 (C3) is a pivotal element of the complement system and 
      plays an important role in innate immunity. A previous study showed that 
      intracellular C3 was upregulated in airway epithelial cells (AECs) from 
      individuals with end-stage chronic obstructive pulmonary disease (COPD). 
      Accumulating evidence has shown that cigarette smoke extract (CSE) induces 
      oxidative stress and apoptosis in AECs. Therefore, we investigated whether C3 
      modulated cigarette smoke-induced oxidative stress and apoptosis in AECs and 
      participated in the pathogenesis of COPD. We found increased C3 expression, 
      together with increased oxidative stress and apoptosis, in a cigarette 
      smoke-induced mouse model of COPD and in AECs from patients with COPD. Different 
      concentrations of CSEinduced C3 expression in 16HBE cells in vitro. 
      Interestingly, C3 knockdown (KD) exacerbated oxidative stress and apoptosis in 
      16HBE cells exposed to CSE. Furthermore, C3 exerted its pro-survival effects 
      through JNK inhibition, while exogenous C3 partially rescued CSE-induced cell 
      death and oxidative stress in C3 KD cells. These data indicate that locally 
      produced C3 is an important pro-survival molecule in AECs under cigarette smoke 
      exposure, revealing a potentially novel mechanism in the pathogenesis of COPD.
CI  - Copyright (c) 2022 Pei, Zhang, Qu, Rao, Li, Gai, Chen, Liang and Sun.
FAU - Pei, Yuqiang
AU  - Pei Y
AD  - Department of Respiratory and Critical Care Medicine, Peking University Third 
      Hospital, Research Center for Chronic Airway Diseases, Peking University Health 
      Science Center, Beijing, China.
FAU - Zhang, Jing
AU  - Zhang J
AD  - Department of Respiratory and Critical Care Medicine, Peking University Third 
      Hospital, Research Center for Chronic Airway Diseases, Peking University Health 
      Science Center, Beijing, China.
FAU - Qu, Jingge
AU  - Qu J
AD  - Department of Respiratory and Critical Care Medicine, Peking University Third 
      Hospital, Research Center for Chronic Airway Diseases, Peking University Health 
      Science Center, Beijing, China.
FAU - Rao, Yafei
AU  - Rao Y
AD  - Department of Respiratory and Critical Care Medicine, Peking University Third 
      Hospital, Research Center for Chronic Airway Diseases, Peking University Health 
      Science Center, Beijing, China.
FAU - Li, Danyang
AU  - Li D
AD  - Department of Respiratory and Critical Care Medicine, Peking University Third 
      Hospital, Research Center for Chronic Airway Diseases, Peking University Health 
      Science Center, Beijing, China.
FAU - Gai, Xiaoyan
AU  - Gai X
AD  - Department of Respiratory and Critical Care Medicine, Peking University Third 
      Hospital, Research Center for Chronic Airway Diseases, Peking University Health 
      Science Center, Beijing, China.
FAU - Chen, Yahong
AU  - Chen Y
AD  - Department of Respiratory and Critical Care Medicine, Peking University Third 
      Hospital, Research Center for Chronic Airway Diseases, Peking University Health 
      Science Center, Beijing, China.
FAU - Liang, Ying
AU  - Liang Y
AD  - Department of Respiratory and Critical Care Medicine, Peking University Third 
      Hospital, Research Center for Chronic Airway Diseases, Peking University Health 
      Science Center, Beijing, China.
FAU - Sun, Yongchang
AU  - Sun Y
AD  - Department of Respiratory and Critical Care Medicine, Peking University Third 
      Hospital, Research Center for Chronic Airway Diseases, Peking University Health 
      Science Center, Beijing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221220
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Complement C3)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Humans
MH  - *Cigarette Smoking/adverse effects
MH  - Complement C3/metabolism
MH  - Oxidative Stress
MH  - *Pulmonary Disease, Chronic Obstructive/pathology
MH  - Epithelial Cells/metabolism
MH  - Apoptosis
MH  - Nicotiana/metabolism
PMC - PMC9807617
OTO - NOTNLM
OT  - apoptosis
OT  - chronic obstructive pulmonary disease
OT  - cigarette smoke
OT  - complement C3
OT  - oxidative stress
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/01/07 06:00
MHDA- 2023/01/10 06:00
PMCR- 2022/01/01
CRDT- 2023/01/06 02:28
PHST- 2022/09/29 00:00 [received]
PHST- 2022/12/06 00:00 [accepted]
PHST- 2023/01/06 02:28 [entrez]
PHST- 2023/01/07 06:00 [pubmed]
PHST- 2023/01/10 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2022.1035930 [doi]
PST - epublish
SO  - Front Immunol. 2022 Dec 20;13:1035930. doi: 10.3389/fimmu.2022.1035930. 
      eCollection 2022.