PMID- 3660586
OWN - NLM
STAT- MEDLINE
DCOM- 19871109
LR  - 20190714
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 160
IP  - 2
DP  - 1987 Oct
TI  - Identification of an amino terminal domain required for the transforming activity 
      of the Rous sarcoma virus src protein.
PG  - 400-10
AB  - Transformation of chicken cells by Rous sarcoma virus (RSV) requires the 
      functional expression of the viral src protein, a tyrosine protein kinase, 
      pp60src. Variants of RSV containing deletions within the amino terminal one-third 
      of the src protein have been identified that exhibit either temperature-sensitive 
      or transformation-defective phenotype when used to infect chicken embryo cells. 
      To define the regions within the amino terminal portion of pp60src that influence 
      morphological transformation, a series of overlapping deletion mutations in the 
      src gene of Prague A RSV (Pr A RSV) were constructed and their biological and 
      biochemical properties were analyzed. Deletions within the src gene which remove 
      amino acid residues 38 to 142 had minimal effects on the ability of the mutant 
      viruses to induce cellular transformation. However, deletions, which impinged 
      upon the region of the src gene encoding residues 142 to 169, inhibited cellular 
      transformation. A variant containing a deletion of amino acid residues 169 to 
      225, was temperature sensitive for transformation. Structurally altered src 
      proteins recovered from cells infected with transformation-defective variants 
      exhibited a somewhat reduced tyrosine protein kinase activities when assayed in 
      the immune complex kinase assay. Analysis of the in vivo phosphorylation of a 
      pp60src substrate, the 36-kDa protein, revealed virtually wild-type levels of 
      phosphorylation in cells infected with the transformation-defective mutants. 
      These studies suggest that the region of the Pr A RSV src protein delineated by 
      amino acid residues 142 to 169 is essential for initiation and maintenance of 
      morphological transformation of chicken cells in culture.
FAU - Raymond, V W
AU  - Raymond VW
AD  - Department of Microbiology, University of Virginia School of Medicine, 
      Charlottesville 22908.
FAU - Parsons, J T
AU  - Parsons JT
LA  - eng
GR  - CA27578/CA/NCI NIH HHS/United States
GR  - CA29243/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Oncogene Proteins, Viral)
RN  - 0 (Phosphoproteins)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Cell Transformation, Viral
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Chromosome Deletion
MH  - Mutation
MH  - Oncogene Proteins, Viral/*physiology
MH  - *Oncogenes
MH  - Peptide Mapping
MH  - Phosphoproteins/metabolism
MH  - Protein-Tyrosine Kinases/metabolism
MH  - Structure-Activity Relationship
EDAT- 1987/10/01 00:00
MHDA- 1987/10/01 00:01
CRDT- 1987/10/01 00:00
PHST- 1987/10/01 00:00 [pubmed]
PHST- 1987/10/01 00:01 [medline]
PHST- 1987/10/01 00:00 [entrez]
AID - 10.1016/0042-6822(87)90011-0 [doi]
PST - ppublish
SO  - Virology. 1987 Oct;160(2):400-10. doi: 10.1016/0042-6822(87)90011-0.