PMID- 36607993
OWN - NLM
STAT- MEDLINE
DCOM- 20230110
LR  - 20230319
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 18
IP  - 1
DP  - 2023
TI  - Metabolomics of a neonatal cohort from the Alliance for Maternal and Newborn 
      Health Improvement biorepository: Effect of preanalytical variables on reference 
      intervals.
PG  - e0279931
LID - 10.1371/journal.pone.0279931 [doi]
LID - e0279931
AB  - BACKGROUND: The study was conducted to determine reference interval (RI) and 
      evaluate the effect of preanalytical variables on Dried blood spot (DBS)-amino 
      acids, acylcarnitines and succinylacetone of neonates. METHODOLOGY: DBS samples 
      were collected within 48-72 hours of life. Samples were analyzed for biochemical 
      markers on tandem mass spectrometer at the University of Iowa. Comparison of RI 
      across various categorical variables were performed. RESULTS: A total of 610 
      reference samples were selected based on exclusion criteria; 53.2% being females. 
      Mean gestational age (GA) of mothers at the time of delivery was 38.7+/-1.6 weeks; 
      24.5% neonates were of low birth weight and 14.3% were preterm. Out of the total 
      610 neonates, 23.1% were small for GA. Reference intervals were generated for 
      eleven amino acids, thirty-two acylcarnitines and succinylacetone concentrations. 
      Markers were evaluated with respect to the influence of gender, GA, weight and 
      time of sampling and statistically significant minimal differences were observed 
      for some biomarkers. CONCLUSION: RI for amino acids, succinylacetone and 
      acylcarnitine on DBS has been established for healthy neonates, which could be of 
      use in the clinical practice. Clinically significant effect of GA, weight, gender 
      and time of sampling on these markers were not identified.
CI  - Copyright: (c) 2023 Jafri et al. This is an open access article distributed under 
      the terms of the Creative Commons Attribution License, which permits unrestricted 
      use, distribution, and reproduction in any medium, provided the original author 
      and source are credited.
FAU - Jafri, Lena
AU  - Jafri L
AUID- ORCID: 0000-0001-6807-5429
AD  - Department of Pathology and Laboratory Medicine, Chemical Pathology, Aga Khan 
      University, Karachi, Pakistan.
FAU - Khan, Aysha Habib
AU  - Khan AH
AD  - Department of Pathology and Laboratory Medicine, Aga Khan University, Karachi, 
      Pakistan.
FAU - Ilyas, Muhammad
AU  - Ilyas M
AD  - Department of Pediatrics and Child Health, Aga Khan University, Karachi, 
      Pakistan.
FAU - Nisar, Imran
AU  - Nisar I
AD  - Department of Pediatrics and Child Health, Aga Khan University, Karachi, 
      Pakistan.
FAU - Khalid, Javairia
AU  - Khalid J
AD  - Department of Pediatrics and Child Health, Aga Khan University, Karachi, 
      Pakistan.
FAU - Majid, Hafsa
AU  - Majid H
AD  - Department of Pathology and Laboratory Medicine, Aga Khan University, Karachi, 
      Pakistan.
FAU - Hotwani, Aneeta
AU  - Hotwani A
AD  - Department of Pediatrics and Child Health, Aga Khan University, Karachi, 
      Pakistan.
FAU - Jehan, Fyezah
AU  - Jehan F
AD  - Department of Pediatrics and Child Health, Aga Khan University, Karachi, 
      Pakistan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230106
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Amino Acids)
RN  - 0 (acylcarnitine)
RN  - S7UI8SM58A (Carnitine)
RN  - 0 (Amines)
SB  - IM
MH  - Infant, Newborn
MH  - Female
MH  - Humans
MH  - Infant
MH  - Male
MH  - *Infant Health
MH  - *Amino Acids
MH  - Carnitine
MH  - Infant, Low Birth Weight
MH  - Amines
PMC - PMC9821480
COIS- The authors have declared that no competing interests exist.
EDAT- 2023/01/07 06:00
MHDA- 2023/01/11 06:00
PMCR- 2023/01/06
CRDT- 2023/01/06 13:44
PHST- 2022/07/06 00:00 [received]
PHST- 2022/12/18 00:00 [accepted]
PHST- 2023/01/06 13:44 [entrez]
PHST- 2023/01/07 06:00 [pubmed]
PHST- 2023/01/11 06:00 [medline]
PHST- 2023/01/06 00:00 [pmc-release]
AID - PONE-D-22-19104 [pii]
AID - 10.1371/journal.pone.0279931 [doi]
PST - epublish
SO  - PLoS One. 2023 Jan 6;18(1):e0279931. doi: 10.1371/journal.pone.0279931. 
      eCollection 2023.