PMID- 36608932
OWN - NLM
STAT- MEDLINE
DCOM- 20230619
LR  - 20231116
IS  - 1673-8527 (Print)
IS  - 1673-8527 (Linking)
VI  - 50
IP  - 6
DP  - 2023 Jun
TI  - Molecular cytogenetic map visualizes the heterozygotic genome and identifies 
      translocation chromosomes in Citrus sinensis.
PG  - 410-421
LID - S1673-8527(22)00283-1 [pii]
LID - 10.1016/j.jgg.2022.12.003 [doi]
AB  - Citrus sinensis is the most cultivated and economically valuable Citrus species 
      in the world, whose genome has been assembled by three generation sequencings. 
      However, chromosome recognition remains a problem due to the small size of 
      chromosomes, and difficulty in differentiating between pseudo and real 
      chromosomes because of a highly heterozygous genome. Here, we employ fluorescence 
      in situ hybridization (FISH) with 9 chromosome painting probes, 30 oligo pools, 
      and 8 repetitive sequences to visualize 18 chromosomes. Then, we develop an 
      approach to identify each chromosome in one cell through single experiment of 
      oligo-FISH and Chromoycin A3 (CMA) staining. By this approach, we construct a 
      high-resolution molecular cytogenetic map containing the physical positions of 
      CMA banding and 38 sequences of FISH including centromere regions, which enables 
      us to visualize significant differences between homologous chromosomes. Based on 
      the map, we locate several highly repetitive sequences on chromosomes and 
      estimate sizes and copy numbers of each site. In particular, we discover the 
      translocation regions of chromosomes 4 and 9 in C. sinensis "Valencia." The 
      high-resolution molecular cytogenetic map will help improve understanding of 
      sweet orange genome assembly and also provide a fundamental reference for 
      investigating chromosome evolution and chromosome engineering for genetic 
      improvement in Citrus.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Song, Shipeng
AU  - Song S
AD  - National Key Laboratory for Germplasm Innovation and Utilization for Fruit and 
      Vegetable Horticultural Crops, Hubei Hongshan Laboratory, Wuhan, Hubei 430070, 
      China; College of Life Science and Technology, Huazhong Agricultural University, 
      Wuhan, Hubei 430070, China.
FAU - Liu, Hui
AU  - Liu H
AD  - National Key Laboratory for Germplasm Innovation and Utilization for Fruit and 
      Vegetable Horticultural Crops, Hubei Hongshan Laboratory, Wuhan, Hubei 430070, 
      China; College of Life Science and Technology, Huazhong Agricultural University, 
      Wuhan, Hubei 430070, China.
FAU - Miao, Luke
AU  - Miao L
AD  - College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 
      Hubei 430070, China.
FAU - He, Li
AU  - He L
AD  - National-local Joint Engineering Laboratory of Citrus Breeding and 
      Cultivation/Horticulture Institute, Sichuan Academy of Agricultural Sciences, 
      Chengdu, Sichuan 610066, China.
FAU - Xie, Wenzhao
AU  - Xie W
AD  - College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 
      Hubei 430070, China; National Key Laboratory of Crop Genetics and Improvement, 
      Huazhong Agricultural University, Wuhan, Hubei 430070, China.
FAU - Lan, Hong
AU  - Lan H
AD  - National Key Laboratory for Germplasm Innovation and Utilization for Fruit and 
      Vegetable Horticultural Crops, Hubei Hongshan Laboratory, Wuhan, Hubei 430070, 
      China; Hubei Province Engineering Research Center of Legume Plants, College of 
      Life Science, Jianghan University, Wuhan, Hubei 430056, China.
FAU - Yu, Changxiu
AU  - Yu C
AD  - National Key Laboratory for Germplasm Innovation and Utilization for Fruit and 
      Vegetable Horticultural Crops, Hubei Hongshan Laboratory, Wuhan, Hubei 430070, 
      China; College of Life Science and Technology, Huazhong Agricultural University, 
      Wuhan, Hubei 430070, China.
FAU - Yan, Wenkai
AU  - Yan W
AD  - State Key Laboratory for Crop Genetics and Germplasm Enhancement, Bioinformatics 
      Center, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural 
      University, Nanjing, Jiangsu 210095, China.
FAU - Wu, Yufeng
AU  - Wu Y
AD  - State Key Laboratory for Crop Genetics and Germplasm Enhancement, Bioinformatics 
      Center, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural 
      University, Nanjing, Jiangsu 210095, China.
FAU - Wen, Xiao-Peng
AU  - Wen XP
AD  - Key Laboratory of Plant Resource Conservation and Germplasm Innovation in 
      Mountainous Region (Ministry of Education), Institute of Agro-bioengineering, 
      College of Life Science, Guizhou University, Guiyang, Guizhou 550025, China.
FAU - Xu, Qiang
AU  - Xu Q
AD  - National Key Laboratory for Germplasm Innovation and Utilization for Fruit and 
      Vegetable Horticultural Crops, Hubei Hongshan Laboratory, Wuhan, Hubei 430070, 
      China.
FAU - Deng, Xiuxin
AU  - Deng X
AD  - National Key Laboratory for Germplasm Innovation and Utilization for Fruit and 
      Vegetable Horticultural Crops, Hubei Hongshan Laboratory, Wuhan, Hubei 430070, 
      China.
FAU - Chen, Chunli
AU  - Chen C
AD  - National Key Laboratory for Germplasm Innovation and Utilization for Fruit and 
      Vegetable Horticultural Crops, Hubei Hongshan Laboratory, Wuhan, Hubei 430070, 
      China; College of Life Science and Technology, Huazhong Agricultural University, 
      Wuhan, Hubei 430070, China. Electronic address: chenchunli@mail.hzau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230104
PL  - China
TA  - J Genet Genomics
JT  - Journal of genetics and genomics = Yi chuan xue bao
JID - 101304616
SB  - IM
MH  - *Citrus sinensis/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - *Citrus/genetics
MH  - Translocation, Genetic/genetics
MH  - Chromosomes, Plant/genetics
OTO - NOTNLM
OT  - CMA banding
OT  - Chromosomal marker
OT  - Chromosomal translocation
OT  - FISH
OT  - Genomic heterozygosity
OT  - Sweet orange
COIS- Conflict of interest The authors declare that there is no conflict of interest.
EDAT- 2023/01/08 06:00
MHDA- 2023/06/19 13:08
CRDT- 2023/01/07 16:06
PHST- 2022/10/24 00:00 [received]
PHST- 2022/12/19 00:00 [revised]
PHST- 2022/12/22 00:00 [accepted]
PHST- 2023/06/19 13:08 [medline]
PHST- 2023/01/08 06:00 [pubmed]
PHST- 2023/01/07 16:06 [entrez]
AID - S1673-8527(22)00283-1 [pii]
AID - 10.1016/j.jgg.2022.12.003 [doi]
PST - ppublish
SO  - J Genet Genomics. 2023 Jun;50(6):410-421. doi: 10.1016/j.jgg.2022.12.003. Epub 
      2023 Jan 4.