PMID- 36610229
OWN - NLM
STAT- MEDLINE
DCOM- 20240123
LR  - 20240123
IS  - 1532-3072 (Electronic)
IS  - 0040-8166 (Linking)
VI  - 81
DP  - 2023 Apr
TI  - MicroRNA-211-5p in extracellular vesicles derived from BMSCs facilitates the 
      repair of rat frozen shoulder via regulating KDM2B/LACC1 axis.
PG  - 102006
LID - S0040-8166(22)00278-6 [pii]
LID - 10.1016/j.tice.2022.102006 [doi]
AB  - OBJECTIVE: This study aims to explore the mechanism of miR-211-5p in 
      extracellular vesicles (EVs) derived from bone marrow mesenchymal stem cells 
      (BMSCs) in improving frozen shoulder (FS) in rat models. METHODS: Rat BMSCs and 
      EVs derived from rat BMSCs were isolated, identified, and then injected into rats 
      to assess the expression of TGF-beta, MMP1, MMP3, MMP12, GAP43, and PGP9.5 in 
      shoulder capsule tissues. The range of motion of bilateral glenohumeral joints 
      was assessed and pathological changes of shoulder capsule tissues were observed 
      after hematoxylin-eosin staining. The binding sites of miR-211-5p to KDM2B and 
      LACC1 to H3K4me3 were measured. FS rat models with LACC1 highly expressed were 
      established to assess the motion of bilateral glenohumeral joints and expression 
      of arthritis related factors in rats. RESULTS: EVs were successfully extracted 
      from BMSCs. Injection of BMSCs-EVs could improve the activity of bilateral 
      glenohumeral joints and the pathological condition of joint capsule in rats. 
      Elevated expression of miR-211-5p was found in rats injected with BMSCs-EVs. Dual 
      luciferase assay showed that miR-211-5p had a binding site with KDM2B. ChIP, 
      qRT-PCR, and western blot experiments showed BMSCs-EVs injection resulted in 
      elevated enrichment of LACC1 promoter in shoulder capsule tissues of FS rats, and 
      decreased mRNA and protein expression of KDM2B and increased H3K4me3 methylation. 
      Overexpression of LACC1 could also improve the pathological condition of joint 
      capsule tissue. CONCLUSION: miR-211-5p in EVs derived from BMSCs increased 
      H3K4me3 methylation in shoulder capsule tissue of rats by binding KDM2B, 
      resulting in up-regulated transcription level of LACC1 and improving FS. 
      AVAILABILITY OF DATA AND MATERIALS: The datasets used or analyzed during the 
      current study are available from the corresponding author on reasonable request.
CI  - Copyright (c) 2023 Elsevier Ltd. All rights reserved.
FAU - Mao, Xiaodong
AU  - Mao X
AD  - Department of Orthopedics & Traumatology, Department of Joint Surgery, Changsha 
      Hospital of Traditional Chinese Medicine, Changsha Eighth Hospital, Changsha, 
      Hunan 410008, PR China.
FAU - Li, Zhi
AU  - Li Z
AD  - Department of Orthopedics & Traumatology, Department of Joint Surgery, Changsha 
      Hospital of Traditional Chinese Medicine, Changsha Eighth Hospital, Changsha, 
      Hunan 410008, PR China.
FAU - Gu, Shaofang
AU  - Gu S
AD  - Department of Orthopedics & Traumatology, Department of Joint Surgery, Changsha 
      Hospital of Traditional Chinese Medicine, Changsha Eighth Hospital, Changsha, 
      Hunan 410008, PR China.
FAU - Song, Wei
AU  - Song W
AD  - Department of Orthopedics & Traumatology, Department of Joint Surgery, Changsha 
      Hospital of Traditional Chinese Medicine, Changsha Eighth Hospital, Changsha, 
      Hunan 410008, PR China.
FAU - Zhang, Mimi
AU  - Zhang M
AD  - Department of Orthopedics & Traumatology, Department of Joint Surgery, Changsha 
      Hospital of Traditional Chinese Medicine, Changsha Eighth Hospital, Changsha, 
      Hunan 410008, PR China.
FAU - Tan, Xiao
AU  - Tan X
AD  - Department of Orthopedics & Traumatology, Department of Joint Surgery, Changsha 
      Hospital of Traditional Chinese Medicine, Changsha Eighth Hospital, Changsha, 
      Hunan 410008, PR China.
FAU - Mao, Ziqing
AU  - Mao Z
AD  - Department of Orthopedics & Traumatology, Department of Joint Surgery, Changsha 
      Hospital of Traditional Chinese Medicine, Changsha Eighth Hospital, Changsha, 
      Hunan 410008, PR China. Electronic address: 329512897@qq.com.
LA  - eng
PT  - Journal Article
DEP - 20221222
PL  - Scotland
TA  - Tissue Cell
JT  - Tissue & cell
JID - 0214745
RN  - 0 (F-Box Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - EC 1.14.11.- (Jumonji Domain-Containing Histone Demethylases)
RN  - 0 (MicroRNAs)
RN  - 0 (MIRN211 microRNA, rat)
RN  - 0 (Lacc1 protein, rat)
RN  - 0 (Kdm2b protein, rat)
SB  - IM
MH  - Animals
MH  - Rats
MH  - *Bursitis/genetics/metabolism
MH  - *Extracellular Vesicles/genetics/metabolism
MH  - F-Box Proteins/genetics/metabolism
MH  - Intracellular Signaling Peptides and Proteins/genetics/metabolism
MH  - Jumonji Domain-Containing Histone Demethylases/genetics/metabolism
MH  - *Mesenchymal Stem Cells/metabolism
MH  - *MicroRNAs/genetics/metabolism
OTO - NOTNLM
OT  - Bone marrow mesenchymal stem cells
OT  - Frozen shoulder
OT  - KDM2B
OT  - LACC1
OT  - MicroRNA-211-5p
COIS- Conflict of interests The authors declare there is no conflict of interests.
EDAT- 2023/01/08 06:00
MHDA- 2023/03/21 06:00
CRDT- 2023/01/07 18:10
PHST- 2022/07/06 00:00 [received]
PHST- 2022/12/21 00:00 [revised]
PHST- 2022/12/21 00:00 [accepted]
PHST- 2023/01/08 06:00 [pubmed]
PHST- 2023/03/21 06:00 [medline]
PHST- 2023/01/07 18:10 [entrez]
AID - S0040-8166(22)00278-6 [pii]
AID - 10.1016/j.tice.2022.102006 [doi]
PST - ppublish
SO  - Tissue Cell. 2023 Apr;81:102006. doi: 10.1016/j.tice.2022.102006. Epub 2022 Dec 
      22.