PMID- 36613292 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20230111 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 12 IP - 1 DP - 2022 Dec 23 TI - Rapid and High-Throughput Determination of Sixteen beta-agonists in Livestock Meat Using One-Step Solid-Phase Extraction Coupled with UHPLC-MS/MS. LID - 10.3390/foods12010076 [doi] LID - 76 AB - beta-agonists are illegally added to animal feed because they can greatly increase carcasses' leanness, which impairs the safety of animal-derived foods and indirectly endangers human health. This study aimed to develop an ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for determining sixteen beta-agonists in livestock meat. The homogenized samples were subjected to enzymatic hydrolysis using beta-glucuronidase/sulfatase at 40 degrees C for 2 h, extracted with acetonitrile containing 1% acetic acid (v/v), and purified by the one-step Qvet-AG extraction column. The residue was redissolved by 0.1% aqueous formic acid/methanol (9:1, v/v) after blow-drying by nitrogen, and then determined by UHPLC-MS/MS. The results demonstrated that the well linearity was in the range of 0.1-50 mug/L with the correlation coefficient (R(2)) >/=0.9928, and the limits of detection (LOD) and quantification (LOQ) were 0.01-0.11 mug/kg and 0.04-0.38 mug/kg, respectively. With intraday and interday relative standard deviations (RSDs) being less than 10%, the average recoveries of pork, beef, and lamb at various spiked levels ranged from 62.62-115.93%, 61.35-106.34%, and 62.00-111.83%, respectively. In conclusion, the established method is simple, efficient, sensitive, and suitable for the simultaneous detection of several beta-agonist residues in livestock meat. FAU - Yan, Yonghong AU - Yan Y AD - College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. AD - National Center of Meat Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China. AD - Key Laboratory of Animal Products Processing, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China. AD - Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China. FAU - Ning, Jun AU - Ning J AD - WENS Foodstuff Group Co., Ltd., Yunfu 527400, China. FAU - Cheng, Xin AU - Cheng X AD - The Center for Agri-Food Quality & Safety, Ministry of Agriculture and Rural Affairs, Beijing 100125, China. FAU - Lv, Qingqin AU - Lv Q AD - College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. AD - National Center of Meat Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China. AD - Key Laboratory of Animal Products Processing, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China. AD - Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China. FAU - Teng, Shuang AU - Teng S AD - College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. AD - National Center of Meat Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China. AD - Key Laboratory of Animal Products Processing, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China. AD - Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China. FAU - Wang, Wei AU - Wang W AUID- ORCID: 0000-0002-1051-6698 AD - College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. AD - National Center of Meat Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China. AD - Key Laboratory of Animal Products Processing, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China. AD - Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China. LA - eng GR - WENS-2020-1-ZDZX-007/Wen's Food Group (China)/ PT - Journal Article DEP - 20221223 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC9818196 OTO - NOTNLM OT - UHPLC-MS/MS OT - livestock meat OT - one-step solid-phase extraction OT - beta-agonist COIS- Author Jun Ning was employed by the company of the WENS Foodstuff Group Co., Ltd. (Guangdong China). The company had the following involvement with the study: software, val-idation, funding and the decision to submit it for publication. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2023/01/09 06:00 MHDA- 2023/01/09 06:01 PMCR- 2022/12/23 CRDT- 2023/01/08 01:17 PHST- 2022/11/11 00:00 [received] PHST- 2022/12/12 00:00 [revised] PHST- 2022/12/21 00:00 [accepted] PHST- 2023/01/08 01:17 [entrez] PHST- 2023/01/09 06:00 [pubmed] PHST- 2023/01/09 06:01 [medline] PHST- 2022/12/23 00:00 [pmc-release] AID - foods12010076 [pii] AID - foods-12-00076 [pii] AID - 10.3390/foods12010076 [doi] PST - epublish SO - Foods. 2022 Dec 23;12(1):76. doi: 10.3390/foods12010076.