PMID- 36618655
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230111
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 13
DP  - 2022
TI  - Transcriptomics-based analysis of the causes of sugar receding in Feizixiao 
      litchi (Litchi chinensis Sonn.) pulp.
PG  - 1083753
LID - 10.3389/fpls.2022.1083753 [doi]
LID - 1083753
AB  - To investigate the causes of the "sugar receding" in 'Feizixiao' litchi (Litchi 
      chinensis Sonn.) pulp, the main sugar contents and sucrose metabolism enzyme 
      activities were measured in pulp obtained in 2020 and 2021. Pulp RNA obtained in 
      2020 was extracted at 35, 63, and 69 days after anthesis (DAA) for transcriptome 
      sequencing analysis. The differential expression of genes was verified by 
      real-time PCR for both years. The results showed that after 63 DAA, the contents 
      of soluble sugars and sucrose decreased, and the contents of fructose and glucose 
      increased in both years. The dynamic changes in sucrose metabolism enzyme 
      activities were similar in both years. After 63 DAA, except for acid invertase 
      (AI) in 2021, the activities of other enzymes decreased significantly, and the 
      net activity of sucrose metabolism enzymes showed a strong sucrose cleavage 
      activity. There were 18061, 19575, and 985 differentially expressed genes in 35 d 
      vs. 63 d, 35 d vs. 69 d, and 63 d vs. 69 d, respectively. Ninety-one sugar 
      metabolism genes were screened out, including sucrose synthase (SS), sucrose 
      phosphate synthase (SPS), AI, neutral invertase (NI), hexokinase (HK), glucose 
      6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), 
      phosphofructokinase (PFK), and pyruvate kinase (PK) genes. In 63 d vs. 69 d, 
      seventy-five percent of sucrose metabolism genes were downregulated, 
      seventy-seven percent of genes in glycolysis (EMP) were upregulated and the PFK 
      genes were significantly upregulated. There was a significant linear correlation 
      between the expression of 15 genes detected by real-time PCR and the 
      transcriptome sequencing results (r(2020) = 0.9139, r(2021) = 0.8912). These 
      results suggest that the upregulated expression of PFK genes at maturity may 
      enhance PFK activity and promote the degradation of soluble sugar in pulp through 
      the EMP pathway, resulting in decreased soluble sugar and sucrose contents and 
      "sugar receding" in pulp. Moreover, the downregulated expression of sucrose 
      metabolism genes in pulp decreased the activities of these enzymes, but the net 
      activity of these enzymes resulted in cleaved sucrose and replenished levels of 
      reducing sugars, resulting in a stable reducing sugar content.
CI  - Copyright (c) 2022 Peng, Du, Wuqiang, Chen, Shui, Liao, Lin and Zhou.
FAU - Peng, Junjie
AU  - Peng J
AD  - Sanya Nanfan Research Institute of Hainan University, Sanya, China.
AD  - College of Horticulture, Hainan University, Haikou, China.
FAU - Du, Jingjia
AU  - Du J
AD  - Sanya Nanfan Research Institute of Hainan University, Sanya, China.
AD  - College of Horticulture, Hainan University, Haikou, China.
FAU - Wuqiang, Ma
AU  - Wuqiang M
AD  - Sanya Nanfan Research Institute of Hainan University, Sanya, China.
AD  - College of Horticulture, Hainan University, Haikou, China.
FAU - Chen, Tiantian
AU  - Chen T
AD  - Sanya Nanfan Research Institute of Hainan University, Sanya, China.
AD  - College of Horticulture, Hainan University, Haikou, China.
FAU - Shui, Xian
AU  - Shui X
AD  - Sanya Nanfan Research Institute of Hainan University, Sanya, China.
AD  - College of Horticulture, Hainan University, Haikou, China.
FAU - Liao, Haizhi
AU  - Liao H
AD  - College of Horticulture, Hainan University, Haikou, China.
FAU - Lin, Xiaokai
AU  - Lin X
AD  - College of Horticulture, Hainan University, Haikou, China.
FAU - Zhou, Kaibing
AU  - Zhou K
AD  - Sanya Nanfan Research Institute of Hainan University, Sanya, China.
AD  - College of Horticulture, Hainan University, Haikou, China.
LA  - eng
PT  - Journal Article
DEP - 20221222
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC9814114
OTO - NOTNLM
OT  - glycolytic pathway
OT  - litchi
OT  - phosphofructokinase
OT  - sugar receding
OT  - transcriptome sequencing
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/01/10 06:00
MHDA- 2023/01/10 06:01
PMCR- 2022/01/01
CRDT- 2023/01/09 03:35
PHST- 2022/11/16 00:00 [received]
PHST- 2022/11/29 00:00 [accepted]
PHST- 2023/01/09 03:35 [entrez]
PHST- 2023/01/10 06:00 [pubmed]
PHST- 2023/01/10 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2022.1083753 [doi]
PST - epublish
SO  - Front Plant Sci. 2022 Dec 22;13:1083753. doi: 10.3389/fpls.2022.1083753. 
      eCollection 2022.