PMID- 36619632
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230111
IS  - 2296-858X (Print)
IS  - 2296-858X (Electronic)
IS  - 2296-858X (Linking)
VI  - 9
DP  - 2022
TI  - Long-term peritoneal dialysate exposure modulates expression of membrane 
      complement regulators in human peritoneal mesothelial cells.
PG  - 972592
LID - 10.3389/fmed.2022.972592 [doi]
LID - 972592
AB  - The membrane complement regulators (CRegs) CD46, CD55, and CD59 are highly 
      expressed on human peritoneal mesothelial cells. However, how mesothelial CRegs 
      change according to the peritoneal dialysis (PD) history of patients has remained 
      unclear. We therefore examined longitudinal changes in CRegs in primary cultured 
      mesothelial cells from PD patients (human peritoneal mesothelial cells; HPMCs) 
      and examined which components of PD fluid (PDF) affect CRegs in vitro. We 
      measured levels of soluble C5b-9 in overnight-dwelling PDF in PD patients and 
      also evaluated changes in CRegs expression on HPMCs collected from PDF using flow 
      cytometry and polymerase chain reaction at a 1-year interval of PD therapy. We 
      also evaluated changes in CReg expressions with stimulation by each component of 
      PDF (glucose, lactic acid and pH) using the Met5A human mesothelial cell line. 
      Levels of sC5b-9 in PDF decreased significantly during 1 year, while expressions 
      of CD46 and CD59 proteins and mRNAs increased significantly in HPMCs during 1 
      year. Analyzing Met-5A cells, we observed that expressions of the three CRegs 
      were increased by glucose and lactic acid in a concentration-dependent manner, 
      but conversely that expressions of CRegs were decreased by lower pH stimulation. 
      History of PD might influence expression of CRegs by HPMCs through properties of 
      PDF such as glucose, lactic acid, and pH. These results suggest that mesothelial 
      cells may alter expression of CRegs for the purpose of protecting the peritoneum 
      and the presence of PDF might affect peritoneal homeostasis associated with the 
      complement system.
CI  - Copyright (c) 2022 Kobayashi, Ozeki, Kim, Imai, Kojima, Iguchi, Fukui, Suzuki, 
      Suzuki, Maruyama, Ito and Mizuno.
FAU - Kobayashi, Kazuma
AU  - Kobayashi K
AD  - Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, 
      Japan.
FAU - Ozeki, Toshikazu
AU  - Ozeki T
AD  - Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, 
      Japan.
FAU - Kim, Hangsoo
AU  - Kim H
AD  - Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, 
      Japan.
AD  - Department of Renal Replacement Therapy, Nagoya University Graduate School of 
      Medicine, Nagoya, Japan.
FAU - Imai, Masaki
AU  - Imai M
AD  - Department of Immunology, Nagoya City University Graduate School of Medicine, 
      Nagoya, Japan.
FAU - Kojima, Hiroshi
AU  - Kojima H
AD  - Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, 
      Japan.
AD  - Department of Renal Replacement Therapy, Nagoya University Graduate School of 
      Medicine, Nagoya, Japan.
FAU - Iguchi, Daiki
AU  - Iguchi D
AD  - Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, 
      Japan.
FAU - Fukui, Sosuke
AU  - Fukui S
AD  - Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, 
      Japan.
AD  - Department of Renal Replacement Therapy, Nagoya University Graduate School of 
      Medicine, Nagoya, Japan.
FAU - Suzuki, Masafumi
AU  - Suzuki M
AD  - Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, 
      Japan.
FAU - Suzuki, Yasuhiro
AU  - Suzuki Y
AD  - Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, 
      Japan.
AD  - Department of Renal Replacement Therapy, Nagoya University Graduate School of 
      Medicine, Nagoya, Japan.
FAU - Maruyama, Shoichi
AU  - Maruyama S
AD  - Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, 
      Japan.
FAU - Ito, Yasuhiko
AU  - Ito Y
AD  - Department of Nephrology and Rheumatology, Aichi Medical University, Nagakute, 
      Japan.
FAU - Mizuno, Masashi
AU  - Mizuno M
AD  - Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, 
      Japan.
AD  - Department of Renal Replacement Therapy, Nagoya University Graduate School of 
      Medicine, Nagoya, Japan.
LA  - eng
PT  - Journal Article
DEP - 20221220
PL  - Switzerland
TA  - Front Med (Lausanne)
JT  - Frontiers in medicine
JID - 101648047
PMC - PMC9815709
OTO - NOTNLM
OT  - complement
OT  - complement regulator
OT  - mesothelial cells
OT  - peritoneal dialysate
OT  - peritoneal dialysis
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/01/10 06:00
MHDA- 2023/01/10 06:01
PMCR- 2022/12/20
CRDT- 2023/01/09 03:55
PHST- 2022/06/18 00:00 [received]
PHST- 2022/11/28 00:00 [accepted]
PHST- 2023/01/09 03:55 [entrez]
PHST- 2023/01/10 06:00 [pubmed]
PHST- 2023/01/10 06:01 [medline]
PHST- 2022/12/20 00:00 [pmc-release]
AID - 10.3389/fmed.2022.972592 [doi]
PST - epublish
SO  - Front Med (Lausanne). 2022 Dec 20;9:972592. doi: 10.3389/fmed.2022.972592. 
      eCollection 2022.