PMID- 36621069
OWN - NLM
STAT- MEDLINE
DCOM- 20230227
LR  - 20230227
IS  - 1879-0909 (Electronic)
IS  - 0141-0229 (Linking)
VI  - 164
DP  - 2023 Mar
TI  - Caffeic acid production from glucose using metabolically engineered Escherichia 
      coli.
PG  - 110193
LID - S0141-0229(23)00001-7 [pii]
LID - 10.1016/j.enzmictec.2023.110193 [doi]
AB  - Caffeic acid (3,4-dihydroxycinnamic acid) is a precursor for high-valued 
      compounds with anticancer, antiviral activities, and anti-inflammatory making it 
      an important substance in the food additive, cosmetics, and pharmaceutical 
      industries. Here, we developed an engineered Escherichia coli strain capable of 
      directly producing high levels of caffeic acid from glucose. Tyrosine 
      ammonia-lyase from Rhodotorula glutinis (RgTAL) and p-coumaric acid 3-hydroxylase 
      from Saccharothrix espanaensis (SeC3H) were expressed. Next, feedback-resistant 
      chorismate mutase/prephenate dehydrogenase, was introduced to promote l-tyrosine 
      synthesis. This engineered strain CA3 produced 1.58 g/L of caffeic acid from 
      glucose without tyrosine supplemented to the medium. Furthermore, to reduce 
      p-coumaric acid accumulation, 4-hydroxyphenylacetate 3-hydroxylase from 
      Pseudomonas aeruginosa (PaHpaBC) was introduced. Finally, an engineered strain 
      CA8 directly produced 6.17 g/L of caffeic acid from glucose using a jar 
      fermenter. The E. coli developed in this study would be helpful as a chassis 
      strain to produce value-added caffeic acid-derivatives.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Sakae, Kosuke
AU  - Sakae K
AD  - Department of Chemical Science and Engineering, Graduate School of Engineering, 
      Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
FAU - Nonaka, Daisuke
AU  - Nonaka D
AD  - Department of Chemical Science and Engineering, Graduate School of Engineering, 
      Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
FAU - Kishida, Mayumi
AU  - Kishida M
AD  - Department of Chemical Science and Engineering, Graduate School of Engineering, 
      Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
FAU - Hirata, Yuuki
AU  - Hirata Y
AD  - Department of Chemical Science and Engineering, Graduate School of Engineering, 
      Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
FAU - Fujiwara, Ryosuke
AU  - Fujiwara R
AD  - Department of Chemical Science and Engineering, Graduate School of Engineering, 
      Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
FAU - Kondo, Akihiko
AU  - Kondo A
AD  - Center for Sustainable Resource Science, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, 
      Yokohama, Kanagawa 230-0045, Japan; Graduate School of Science, Technology and 
      Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
FAU - Noda, Shuhei
AU  - Noda S
AD  - Center for Sustainable Resource Science, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, 
      Yokohama, Kanagawa 230-0045, Japan.
FAU - Tanaka, Tsutomu
AU  - Tanaka T
AD  - Department of Chemical Science and Engineering, Graduate School of Engineering, 
      Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan. Electronic address: 
      tanaka@kitty.kobe-u.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20230105
PL  - United States
TA  - Enzyme Microb Technol
JT  - Enzyme and microbial technology
JID - 8003761
RN  - U2S3A33KVM (caffeic acid)
RN  - IY9XDZ35W2 (Glucose)
RN  - EC 1.- (Mixed Function Oxygenases)
RN  - IBS9D1EU3J (p-coumaric acid)
RN  - 0 (Caffeic Acids)
SB  - IM
MH  - *Escherichia coli/genetics/metabolism
MH  - *Glucose
MH  - *Metabolic Engineering
MH  - Mixed Function Oxygenases
MH  - *Caffeic Acids/metabolism
OTO - NOTNLM
OT  - Caffeic acid
OT  - E. coli
OT  - Metabolic engineering
OT  - Shikimate pathway
OT  - p-coumaric acid
COIS- Conflicts of interest The authors declare no commercial or financial conflict of 
      interest.
EDAT- 2023/01/10 06:00
MHDA- 2023/01/25 06:00
CRDT- 2023/01/09 04:30
PHST- 2022/09/15 00:00 [received]
PHST- 2023/01/03 00:00 [revised]
PHST- 2023/01/04 00:00 [accepted]
PHST- 2023/01/10 06:00 [pubmed]
PHST- 2023/01/25 06:00 [medline]
PHST- 2023/01/09 04:30 [entrez]
AID - S0141-0229(23)00001-7 [pii]
AID - 10.1016/j.enzmictec.2023.110193 [doi]
PST - ppublish
SO  - Enzyme Microb Technol. 2023 Mar;164:110193. doi: 10.1016/j.enzmictec.2023.110193. 
      Epub 2023 Jan 5.