PMID- 36623874
OWN - NLM
STAT- MEDLINE
DCOM- 20230222
LR  - 20240214
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 43
IP  - 7
DP  - 2023 Feb 15
TI  - Arc-Expressing Accessory Olfactory Bulb Interneurons Support Chemosensory Social 
      Behavioral Plasticity.
PG  - 1178-1190
LID - 10.1523/JNEUROSCI.0847-22.2022 [doi]
AB  - The accessory olfactory system (AOS) is critical for the development and 
      expression of social behavior. The first dedicated circuit in the AOS, the 
      accessory olfactory bulb (AOB), exhibits cellular and network plasticity in male 
      and female mice after social experience. In the AOB, interneurons called internal 
      granule cells (IGCs) express the plasticity-associated immediate-early gene Arc 
      following intermale aggression or mating. Here, we sought to better understand 
      how Arc-expressing IGCs shape AOB information processing and social behavior in 
      the context of territorial aggression. We used "ArcTRAP" (Arc-CreERT2) transgenic 
      mice to selectively and permanently label Arc-expressing IGCs following male-male 
      resident-intruder interactions. Using whole-cell patch-clamp electrophysiology, 
      we found that Arc-expressing IGCs display increased intrinsic excitability for 
      several days after a single resident-intruder interaction. Further, we found that 
      Arc-expressing IGCs maintain this increased excitability across repeated 
      resident-intruder interactions, during which resident mice increase or "ramp" 
      their aggression. We tested the hypothesis that Arc-expressing IGCs participate 
      in ramping aggression. Using a combination of ArcTRAP mice and chemogenetics 
      (Cre-dependent hM4D(G(i))-mCherry AAV injections), we found that disruption of 
      Arc-expressing IGC activity during repeated resident-intruder interactions 
      abolishes the ramping aggression exhibited by resident male mice. This work shows 
      that Arc-expressing AOB IGC ensembles are activated by specific chemosensory 
      environments, and play an integral role in the establishment and expression of 
      sex-typical social behavior. These studies identify a population of plastic 
      interneurons in an early chemosensory circuit that display physiological features 
      consistent with simple memory formation, increasing our understanding of central 
      chemosensory processing and mammalian social behavior.SIGNIFICANCE STATEMENT The 
      accessory olfactory system plays a vital role in rodent chemosensory social 
      behavior. We studied experience-dependent plasticity in the accessory olfactory 
      bulb and found that internal granule cells expressing the immediate-early gene 
      Arc after the resident-intruder paradigm increase their excitability for several 
      days. We investigated the roles of these Arc-expressing internal granule cells on 
      chemosensory social behavior by chemogenetically manipulating their excitability 
      during repeated social interactions. We found that inhibiting these cells 
      eliminated intermale aggressive ramping behavior. These studies identify a 
      population of plastic interneurons in an early chemosensory circuit that display 
      physiological features consistent with simple memory formation, increasing our 
      understanding of central chemosensory processing and mammalian social behavior.
CI  - Copyright (c) 2023 Zuk et al.
FAU - Zuk, Kelsey E
AU  - Zuk KE
AD  - Neuroscience Graduate Program, University of Texas Southwestern Medical Center, 
      Dallas, Texas 75390.
AD  - Department of Neuroscience, University of Rochester School of Medicine and 
      Dentistry, Rochester, New York 14642.
FAU - Cansler, Hillary L
AU  - Cansler HL
AUID- ORCID: 0000-0001-8267-0515
AD  - Department of Pharmacology, University of Florida College of Medicine, 
      Gainesville, Florida 32603.
FAU - Wang, Jinxin
AU  - Wang J
AD  - Department of Neuroscience, University of Rochester School of Medicine and 
      Dentistry, Rochester, New York 14642.
FAU - Meeks, Julian P
AU  - Meeks JP
AUID- ORCID: 0000-0002-7537-4491
AD  - Department of Neuroscience, University of Rochester School of Medicine and 
      Dentistry, Rochester, New York 14642 julian_meeks@urmc.rochester.edu.
AD  - Department of Pediatrics, University of Rochester School of Medicine and 
      Dentistry, Rochester, New York 14642.
LA  - eng
GR  - F31 DC018440/DC/NIDCD NIH HHS/United States
GR  - R01 DC015784/DC/NIDCD NIH HHS/United States
GR  - R01 DC017985/DC/NIDCD NIH HHS/United States
GR  - R21 NS104826/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20230109
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
SB  - IM
MH  - Mice
MH  - Male
MH  - Female
MH  - Animals
MH  - *Olfactory Bulb/physiology
MH  - *Interneurons/physiology
MH  - Neurons
MH  - Social Behavior
MH  - Aggression
MH  - Mice, Transgenic
MH  - Mammals
PMC - PMC9962775
OTO - NOTNLM
OT  - Arc
OT  - accessory olfactory bulb
OT  - aggression
OT  - interneuron
OT  - plasticity
OT  - social behavior
EDAT- 2023/01/10 06:00
MHDA- 2023/02/22 06:00
PMCR- 2023/02/15
CRDT- 2023/01/09 20:52
PHST- 2022/05/02 00:00 [received]
PHST- 2022/11/18 00:00 [revised]
PHST- 2022/11/22 00:00 [accepted]
PHST- 2023/01/10 06:00 [pubmed]
PHST- 2023/02/22 06:00 [medline]
PHST- 2023/01/09 20:52 [entrez]
PHST- 2023/02/15 00:00 [pmc-release]
AID - JNEUROSCI.0847-22.2022 [pii]
AID - JN-RM-0847-22 [pii]
AID - 10.1523/JNEUROSCI.0847-22.2022 [doi]
PST - ppublish
SO  - J Neurosci. 2023 Feb 15;43(7):1178-1190. doi: 10.1523/JNEUROSCI.0847-22.2022. 
      Epub 2023 Jan 9.