PMID- 36625073
OWN - NLM
STAT- MEDLINE
DCOM- 20230202
LR  - 20230402
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 27
IP  - 3
DP  - 2023 Feb
TI  - Fluorescence in situ hybridization test for detection of endometrial carcinoma 
      cells by non-invasive vaginal swab.
PG  - 379-391
LID - 10.1111/jcmm.17658 [doi]
AB  - Endometrial cancer (EC) is the most common gynaecological malignancy with 
      increasing incidence in developed countries. As gold standard, hysteroscopy 
      confirms only 30% of suspected ECs. The detection of EC cells in the vagina by 
      fluorescence in situ hybridization (FISH) after a smear test could reduce 
      invasive procedures in the future. Using array-based comparative genome 
      hybridization (aCGH) on 65 endometrial carcinomas, most frequently imbalanced 
      regions of the tumour genome were identified. Bacterial artificial chromosomes 
      were used to generate FISH-probes homologue to these human regions. The FISH test 
      was hybridized on swabs specimens collected from the vaginal cavity. Samples from 
      six patients without EC were selected as a negative control and on 13 patients 
      with known EC as a positive control. To distinguish between benign and EC cases, 
      the cut-off value has been defined. A first validation of this EC-FISH Test was 
      performed with swabs from 41 patients with suspected EC. The most common genomic 
      imbalances in EC are around the CTNNB1, FBXW7 and APC genes. The cut-off is 
      defined at 32% of analysed cells without diploid signal pattern. This differs 
      significantly between the positive and negative controls (p < 0.001). In a first 
      validation cohort of 41 patients with suspected EC, the EC-FISH Test 
      distinguishes patients with and without EC with a sensitivity of 91% and a 
      specificity of 83%. The negative predictive value is 96%. This is the first 
      report of a non-invasive EC-FISH Test to predict EC in women with suspected EC.
CI  - (c) 2023 The Authors. Journal of Cellular and Molecular Medicine published by 
      Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
FAU - Weimer, Jorg
AU  - Weimer J
AUID- ORCID: 0000-0002-4528-8509
AD  - Department of Gynecology and Obstetrics, Christian-Albrechts-University Kiel and 
      University Medical Center Schleswig-Holstein Campus Kiel, Kiel, Germany.
FAU - Huttmann, Martje
AU  - Huttmann M
AD  - Department of Gynecology and Obstetrics, Christian-Albrechts-University Kiel and 
      University Medical Center Schleswig-Holstein Campus Kiel, Kiel, Germany.
FAU - Nusilati, Asiyan
AU  - Nusilati A
AD  - Department of Gynecology and Obstetrics, Christian-Albrechts-University Kiel and 
      University Medical Center Schleswig-Holstein Campus Kiel, Kiel, Germany.
FAU - Andreas, Svenja
AU  - Andreas S
AD  - Department of Gynecology and Obstetrics, Christian-Albrechts-University Kiel and 
      University Medical Center Schleswig-Holstein Campus Kiel, Kiel, Germany.
FAU - Roseler, Jona
AU  - Roseler J
AD  - Department of Gynecology and Obstetrics, Christian-Albrechts-University Kiel and 
      University Medical Center Schleswig-Holstein Campus Kiel, Kiel, Germany.
FAU - Tribian, Nils
AU  - Tribian N
AD  - Department of Gynecology and Obstetrics, Christian-Albrechts-University Kiel and 
      University Medical Center Schleswig-Holstein Campus Kiel, Kiel, Germany.
FAU - Rogmans, Christoph
AU  - Rogmans C
AD  - Department of Gynecology and Obstetrics, Christian-Albrechts-University Kiel and 
      University Medical Center Schleswig-Holstein Campus Kiel, Kiel, Germany.
FAU - Stope, Matthias Bernhard
AU  - Stope MB
AD  - Department of Gynecology and Gynecological Oncology, University Hospital Bonn, 
      Bonn, Germany.
FAU - Dahl, Edgar
AU  - Dahl E
AD  - Institute of Pathology, Medical Faculty of RWTH Aachen University, Aachen, 
      Germany.
FAU - Mustea, Alexander
AU  - Mustea A
AD  - Department of Gynecology and Gynecological Oncology, University Hospital Bonn, 
      Bonn, Germany.
FAU - Stickeler, Elmar
AU  - Stickeler E
AD  - Department of Gynecology, University Medical Center RWTH Aachen, Aachen, Germany.
FAU - Hedemann, Nina
AU  - Hedemann N
AD  - Department of Gynecology and Obstetrics, Christian-Albrechts-University Kiel and 
      University Medical Center Schleswig-Holstein Campus Kiel, Kiel, Germany.
FAU - Florkemeier, Inken
AU  - Florkemeier I
AD  - Department of Gynecology and Obstetrics, Christian-Albrechts-University Kiel and 
      University Medical Center Schleswig-Holstein Campus Kiel, Kiel, Germany.
FAU - Tiemann, Katharina
AU  - Tiemann K
AD  - Institute for Hematopathology, Hamburg, Germany.
FAU - Magadeeva, Svetlana
AU  - Magadeeva S
AD  - Department of Gynecology and Obstetrics, Christian-Albrechts-University Kiel and 
      University Medical Center Schleswig-Holstein Campus Kiel, Kiel, Germany.
FAU - Dempfle, Astrid
AU  - Dempfle A
AD  - Institute of Medical Informatics and Statistics, Kiel University and University 
      Medical Center Schleswig-Holstein Campus Kiel, Kiel, Germany.
FAU - Arnold, Norbert
AU  - Arnold N
AD  - Department of Gynecology and Obstetrics, Christian-Albrechts-University Kiel and 
      University Medical Center Schleswig-Holstein Campus Kiel, Kiel, Germany.
FAU - Maass, Nicolai
AU  - Maass N
AD  - Department of Gynecology and Obstetrics, Christian-Albrechts-University Kiel and 
      University Medical Center Schleswig-Holstein Campus Kiel, Kiel, Germany.
FAU - Bauerschlag, Dirk
AU  - Bauerschlag D
AD  - Department of Gynecology and Obstetrics, Christian-Albrechts-University Kiel and 
      University Medical Center Schleswig-Holstein Campus Kiel, Kiel, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230110
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
SB  - IM
MH  - Humans
MH  - Female
MH  - Sensitivity and Specificity
MH  - In Situ Hybridization, Fluorescence
MH  - *Endometrial Neoplasms/diagnosis/genetics/pathology
MH  - Predictive Value of Tests
MH  - Vagina
PMC - PMC9889703
OTO - NOTNLM
OT  - FISH
OT  - endometrial-cancer
OT  - non-invasive
OT  - predictive-marker
OT  - vaginal-swap
COIS- The authors declare that there is no conflict of interest.
EDAT- 2023/01/11 06:00
MHDA- 2023/02/03 06:00
PMCR- 2023/01/10
CRDT- 2023/01/10 03:12
PHST- 2022/10/19 00:00 [revised]
PHST- 2022/06/21 00:00 [received]
PHST- 2022/12/10 00:00 [accepted]
PHST- 2023/01/11 06:00 [pubmed]
PHST- 2023/02/03 06:00 [medline]
PHST- 2023/01/10 03:12 [entrez]
PHST- 2023/01/10 00:00 [pmc-release]
AID - JCMM17658 [pii]
AID - 10.1111/jcmm.17658 [doi]
PST - ppublish
SO  - J Cell Mol Med. 2023 Feb;27(3):379-391. doi: 10.1111/jcmm.17658. Epub 2023 Jan 
      10.