PMID- 36625895
OWN - NLM
STAT- MEDLINE
DCOM- 20230131
LR  - 20240222
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Print)
IS  - 1618-2642 (Linking)
VI  - 415
IP  - 5
DP  - 2023 Feb
TI  - Label-free sub-micrometer 3D imaging of ciprofloxacin in native-state biofilms 
      with cryo-time-of-flight secondary ion mass spectrometry.
PG  - 991-999
LID - 10.1007/s00216-022-04496-4 [doi]
AB  - High spatial resolution mass spectrometry imaging has been identified as a key 
      technology needed to improve understanding of the chemical components that 
      influence antibiotic resistance within biofilms, which are communities of 
      micro-organisms that grow attached to a surface. Time-of-flight secondary ion 
      mass spectrometry (ToF-SIMS) offers the unique ability for label-free 3D imaging 
      of organic molecules with sub-micrometer spatial resolution and high sensitivity. 
      Several studies of biofilms have been done with the help of ToF-SIMS, but none of 
      those studies have shown 3D imaging of antibiotics in native-state hydrated 
      biofilms with cell-level resolution. Because ToF-SIMS measurements must be 
      performed in a high-vacuum environment, cryogenic preparation and analysis are 
      necessary to preserve the native biofilm structure and antibiotic spatial 
      distribution during ToF-SIMS measurements. In this study, we have investigated 
      the penetration of the antibiotic ciprofloxacin into Bacillus subtilis biofilms 
      using sub-micrometer resolution 3D imaging cryo-ToF-SIMS. B. subtilis biofilms 
      were exposed to physiologically relevant levels of ciprofloxacin. The treated 
      biofilms were then plunge-frozen in liquid propane and analyzed with ToF-SIMS 
      under cryogenic conditions. Multivariate analysis techniques, including 
      multivariate curve resolution (MCR) and inverse maximum signal factor (iMSF) 
      denoising, were used to aid analysis of the data and facilitate high spatial 
      resolution 3D imaging of the biofilm, providing individually resolved cells and 
      spatially resolved ciprofloxacin intensity at "real world" concentrations.
CI  - (c) 2023. The Author(s).
FAU - Akbari, Anoosheh
AU  - Akbari A
AD  - Physikalisches Institut and Center for Soft Nanoscience, University of Munster, 
      Wilhelm-Klemm-Strasse 10, 48149, Munster, Germany.
FAU - Galstyan, Anzhela
AU  - Galstyan A
AD  - Department of Chemistry, Center for Nanointegration Duisburg-Essen (CENIDE) and 
      Centre for Water and Environmental Research (ZWU), University of Duisburg-Essen, 
      Universitatsstrasse 5, 45141, Essen, Germany.
FAU - Peterson, Richard E
AU  - Peterson RE
AD  - Physikalisches Institut and Center for Soft Nanoscience, University of Munster, 
      Wilhelm-Klemm-Strasse 10, 48149, Munster, Germany.
FAU - Arlinghaus, Heinrich F
AU  - Arlinghaus HF
AD  - Physikalisches Institut and Center for Soft Nanoscience, University of Munster, 
      Wilhelm-Klemm-Strasse 10, 48149, Munster, Germany.
FAU - Tyler, Bonnie J
AU  - Tyler BJ
AD  - Physikalisches Institut and Center for Soft Nanoscience, University of Munster, 
      Wilhelm-Klemm-Strasse 10, 48149, Munster, Germany. tyler@uni-muenster.de.
LA  - eng
PT  - Journal Article
DEP - 20230110
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 5E8K9I0O4U (Ciprofloxacin)
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - *Spectrometry, Mass, Secondary Ion/methods
MH  - *Imaging, Three-Dimensional
MH  - Ciprofloxacin
MH  - Biofilms
MH  - Anti-Bacterial Agents
PMC - PMC9883301
OTO - NOTNLM
OT  - 3D imaging
OT  - Antibiotic
OT  - Biofilm
OT  - Cryogenic analysis
OT  - Multivariate analysis
OT  - ToF-SIMS
COIS- The authors declare no competing interests.
EDAT- 2023/01/11 06:00
MHDA- 2023/02/01 06:00
PMCR- 2023/01/10
CRDT- 2023/01/10 11:14
PHST- 2022/08/11 00:00 [received]
PHST- 2022/12/19 00:00 [accepted]
PHST- 2022/11/25 00:00 [revised]
PHST- 2023/01/11 06:00 [pubmed]
PHST- 2023/02/01 06:00 [medline]
PHST- 2023/01/10 11:14 [entrez]
PHST- 2023/01/10 00:00 [pmc-release]
AID - 10.1007/s00216-022-04496-4 [pii]
AID - 4496 [pii]
AID - 10.1007/s00216-022-04496-4 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2023 Feb;415(5):991-999. doi: 10.1007/s00216-022-04496-4. Epub 
      2023 Jan 10.