PMID- 36626243
OWN - NLM
STAT- MEDLINE
DCOM- 20230124
LR  - 20231124
IS  - 1945-4589 (Electronic)
IS  - 1945-4589 (Linking)
VI  - 15
IP  - 1
DP  - 2023 Jan 9
TI  - Mitoquinone shifts energy metabolism to reduce ROS-induced oxeiptosis in female 
      granulosa cells and mouse oocytes.
PG  - 246-260
LID - 10.18632/aging.204475 [doi]
AB  - The female reproductive system is quite sensitive to regulation, and external 
      environmental stimuli may cause oxidative stress which in turn may lead to 
      accelerated aging and programmed cell death in female reproductive cells. The aim 
      of this study was to investigate whether or not mitoquinone (MitoQ) could resist 
      ROS-induced apoptosis in human granulosa cells and mouse oocytes. We found that 
      the MitoQ treatment significantly reduced production of reactive oxygen species 
      (ROS) and imbalance in mitochondrial membrane potential. The MitoQ treatment 
      prevented an excessive mitochondrial fragmentation by upregulating Drp1 S637 and 
      decreasing Drp1 S637 phosphorylation. More importantly, MitoQ maintained aerobic 
      respiration and reduced anaerobic respiration by regulating reprogramming of 
      intracellular energy metabolism, which enhanced cellular ATP production. MitoQ 
      effectively reduced the expressions of AIFM1 and PGAM5, key molecules whose 
      expressions were reversed not only in granulosa cells but also in mouse oocytes. 
      Our findings suggest that MitoQ can ameliorate the mitochondrial deterioration 
      caused by ROS and reprogram cellular energy metabolism, providing protection to 
      cells against apoptosis. The presence of MitoQ may help in protecting human germ 
      cells under in vitro culture conditions.
FAU - Tsui, Kuan-Hao
AU  - Tsui KH
AD  - Department of Obstetrics and Gynaecology, Kaohsiung Veterans General Hospital, 
      Kaohsiung 813, Taiwan.
AD  - Institute of Biopharmaceutical Sciences, National Sun Yat-Sen University, 
      Kaohsiung 804, Taiwan.
AD  - Department of Obstetrics and Gynaecology, National Yang-Ming University School of 
      Medicine, Taipei 112, Taiwan.
AD  - Department of Obstetrics and Gynecology, Taipei Veterans General Hospital, Taipei 
      112, Taiwan.
AD  - Department of Pharmacy and Master Program, College of Pharmacy and Health Care, 
      Tajen University, Pingtung County 907, Taiwan.
AD  - Department of Medicine, Tri-Service General Hospital, National Defense Medical 
      Center, Taipei 114, Taiwan.
AD  - College of Health and Nursing, Meiho University, Pingtung County 912, Taiwan.
FAU - Li, Chia-Jung
AU  - Li CJ
AD  - Department of Obstetrics and Gynaecology, Kaohsiung Veterans General Hospital, 
      Kaohsiung 813, Taiwan.
AD  - Institute of Biopharmaceutical Sciences, National Sun Yat-Sen University, 
      Kaohsiung 804, Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230109
PL  - United States
TA  - Aging (Albany NY)
JT  - Aging
JID - 101508617
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Antioxidants)
SB  - IM
MH  - Mice
MH  - Female
MH  - Humans
MH  - Animals
MH  - Reactive Oxygen Species/metabolism
MH  - *Antioxidants/pharmacology
MH  - *Oocytes/metabolism
MH  - Energy Metabolism
MH  - Granulosa Cells/metabolism
PMC - PMC9876626
OTO - NOTNLM
OT  - MitoQ
OT  - ROS
OT  - metabolism
OT  - mitochondria
OT  - oxeiptosis
COIS- CONFLICTS OF INTEREST: The authors declare no conflicts of interest related to 
      this study.
EDAT- 2023/01/11 06:00
MHDA- 2023/01/25 06:00
PMCR- 2023/01/15
CRDT- 2023/01/10 12:22
PHST- 2022/08/30 00:00 [received]
PHST- 2022/12/16 00:00 [accepted]
PHST- 2023/01/11 06:00 [pubmed]
PHST- 2023/01/25 06:00 [medline]
PHST- 2023/01/10 12:22 [entrez]
PHST- 2023/01/15 00:00 [pmc-release]
AID - 204475 [pii]
AID - 10.18632/aging.204475 [doi]
PST - ppublish
SO  - Aging (Albany NY). 2023 Jan 9;15(1):246-260. doi: 10.18632/aging.204475. Epub 
      2023 Jan 9.