PMID- 36626597
OWN - NLM
STAT- MEDLINE
DCOM- 20230801
LR  - 20230907
IS  - 1529-4242 (Electronic)
IS  - 0032-1052 (Linking)
VI  - 152
IP  - 2
DP  - 2023 Aug 1
TI  - Early Angiogenesis-Dependent CXCL12 Attracts Adipose-Derived Stem Cells to 
      Promote the Repair of Fat Grafting in a Mouse Model.
PG  - 363-372
LID - 10.1097/PRS.0000000000010186 [doi]
AB  - BACKGROUND: The unpredictable and unstable tissue retention rate of autologous 
      fat grafting remains an obstacle faced by plastic surgeons. The authors' previous 
      study using a fat grafting mouse model with donor sites showed that 
      adipose-derived stem cell (ASC) infiltration in the recipient site was delayed, 
      leading to poor regeneration and lower retention. Thus, the mechanism behind the 
      differential infiltration of ASCs needed to be explored. METHODS: First, the 
      authors locally injected C-X-C chemokine ligand 12 (CXCL12) or C-X-C motif 
      chemokine receptor 4 (CXCR4) inhibitor AMD3100 in the recipient or donor site, 
      respectively (CXCL12 + AMD3100 - , CXCL12 - AMD3100 + , and CXCL12 + AMD3100 + 
      groups). The authors compared the migration of ASCs, adipose regeneration, and 
      long-term retention. Next, the authors explored the role of angiogenesis using a 
      normal/ischemic mice model in which the authors test the expression of 
      CXCL12/CXCR4, migration of ASCs, and adipose regeneration. RESULTS: Blocking 
      CXCL12 in the donor site using AMD3100 (CXCL12 - AMD3100 + and CXCL12+AMD3100+ 
      groups) could accelerate ASC infiltration and promote adipose regeneration and 
      long-term retention ( P < 0.05) compared with the other groups. CXCL12 and its 
      receptor CXCR4 were more highly expressed in normal than in ischemic adipose 
      tissue; consistently, there were more ASCs infiltrating normal than ischemic 
      adipose tissue early after surgery ( P < 0.05). CONCLUSION: Early angiogenesis is 
      essential for CXCL12 in promoting ASC infiltration, improving adipose tissue 
      repair in the recipient site, and potentiating the long-term fat retention rate. 
      CLINICAL RELEVANCE STATEMENT: The authors provide a proof-of-concept way to 
      improve the outcomes of fat grafting by locally injecting AMD3100, also known as 
      plerixafor, to the donor site.
CI  - Copyright (c) 2023 by the American Society of Plastic Surgeons.
FAU - Chen, Yunzi
AU  - Chen Y
AD  - From the Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern 
      Medical University.
FAU - Wang, Zijue
AU  - Wang Z
AD  - From the Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern 
      Medical University.
FAU - Li, Ye
AU  - Li Y
AD  - From the Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern 
      Medical University.
FAU - Li, Bin
AU  - Li B
AD  - From the Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern 
      Medical University.
FAU - Wang, Xinhui
AU  - Wang X
AD  - From the Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern 
      Medical University.
FAU - Zou, Jialiang
AU  - Zou J
AD  - From the Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern 
      Medical University.
FAU - Bi, Xin
AU  - Bi X
AD  - From the Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern 
      Medical University.
FAU - Zhao, Jing
AU  - Zhao J
AD  - From the Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern 
      Medical University.
FAU - Lu, Feng
AU  - Lu F
AD  - From the Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern 
      Medical University.
FAU - Dong, Ziqing
AU  - Dong Z
AD  - From the Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern 
      Medical University.
LA  - eng
GR  - 2020M672723/Postdoctoral Science Foundation of China/
GR  - A2020542/Medical Scientific Research Foundation of Guangdong Province of China/
GR  - 201604020007/Science and Technology Program of Guangzhou of China/
GR  - 2019B021/Administrator Foundation of Nanfang Hospital (CN)/
GR  - X202012121222/National undergraduate innovation and entrepreneurship training 
      program/
GR  - X202012121312/National undergraduate innovation and entrepreneurship training 
      program/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230727
PL  - United States
TA  - Plast Reconstr Surg
JT  - Plastic and reconstructive surgery
JID - 1306050
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Heterocyclic Compounds)
RN  - 0 (Ligands)
RN  - S915P5499N (plerixafor)
RN  - 0 (Cxcl12 protein, mouse)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Adipose Tissue/metabolism
MH  - Chemokine CXCL12/metabolism
MH  - *Hematopoietic Stem Cell Mobilization
MH  - *Heterocyclic Compounds/pharmacology/metabolism
MH  - Ligands
MH  - Stem Cells/metabolism
EDAT- 2023/01/11 06:00
MHDA- 2023/07/31 06:42
CRDT- 2023/01/10 14:32
PHST- 2023/07/31 06:42 [medline]
PHST- 2023/01/11 06:00 [pubmed]
PHST- 2023/01/10 14:32 [entrez]
AID - 00006534-202308000-00023 [pii]
AID - 10.1097/PRS.0000000000010186 [doi]
PST - ppublish
SO  - Plast Reconstr Surg. 2023 Aug 1;152(2):363-372. doi: 
      10.1097/PRS.0000000000010186. Epub 2023 Jul 27.