PMID- 36629523
OWN - NLM
STAT- MEDLINE
DCOM- 20230113
LR  - 20230113
IS  - 1414-431X (Electronic)
IS  - 0100-879X (Print)
IS  - 0100-879X (Linking)
VI  - 55
DP  - 2023
TI  - Down-regulation of autophagy proteins is associated with higher mTOR expression 
      in the placenta of pregnant women with preeclampsia.
PG  - e12283
LID - S0100-879X2022000100683 [pii]
LID - 10.1590/1414-431X2022e12283 [doi]
LID - e12283
AB  - Autophagy is a lysosomal degradation pathway that removes protein aggregates and 
      damaged organelles maintaining cellular integrity. It seems to be essential for 
      cell survival during stress, starvation, hypoxia, and consequently to the 
      placenta implantation and development. Preeclampsia (PE) is a multisystemic 
      disorder characterized by the onset of hypertension associated or not with 
      proteinuria and other maternal complications. Considering that the placenta seems 
      to play an important role in the pathogenesis of PE, the objective of the present 
      study was to evaluate protein levels of light chain protein (LC3), beclin-1, and 
      the mammalian target of rapamycin (mTOR) in the placenta of pregnant women with 
      PE. Placental tissues collected from 20 women with PE and 20 normotensive (NT) 
      pregnant women were evaluated for LC3, beclin-1, and mTOR expression by qPCR and 
      immunohistochemistry. The mRNA for LC3 and beclin-1 were significantly lower, 
      while mTOR gene expression was significantly higher in the placenta of pregnant 
      women with PE than in the NT group. Placentas of PE women showed significantly 
      decreased protein expression of LC3-II and beclin-1, whereas mTOR was 
      significantly increased compared with the NT pregnant women. There was a negative 
      correlation between protein expression of mTOR and LC3-II in the placental tissue 
      of PE women. In conclusion, the results showed autophagy deficiency suggesting 
      that failure in this degradation process may contribute to the pathogenesis of 
      PE; however, new studies involving cross-talk between autophagy and inflammatory 
      molecular mechanisms might help to better understand the autophagy process in 
      this obstetric pathology.
FAU - Weel, I C
AU  - Weel IC
AUID- ORCID: 0000-0002-8267-5911
AD  - Departamento de Ciencias Quimicas e Biologicas, Instituto de Biociencias, 
      Universidade Estadual Paulista, Botucatu, SP, Brasil.
FAU - Ribeiro, V R
AU  - Ribeiro VR
AUID- ORCID: 0000-0001-7629-7689
AD  - Departamento de Ciencias Quimicas e Biologicas, Instituto de Biociencias, 
      Universidade Estadual Paulista, Botucatu, SP, Brasil.
FAU - Romao-Veiga, M
AU  - Romao-Veiga M
AUID- ORCID: 0000-0002-8990-0237
AD  - Departamento de Ciencias Quimicas e Biologicas, Instituto de Biociencias, 
      Universidade Estadual Paulista, Botucatu, SP, Brasil.
FAU - Fioratti, E G
AU  - Fioratti EG
AUID- ORCID: 0000-0002-1982-0119
AD  - Departamento de Ciencias Quimicas e Biologicas, Instituto de Biociencias, 
      Universidade Estadual Paulista, Botucatu, SP, Brasil.
FAU - Peracoli, J C
AU  - Peracoli JC
AUID- ORCID: 0000-0002-3273-3001
AD  - Departamento de Ginecologia e Obstetricia, Faculdade de Medicina de Botucatu, 
      Universidade Estadual Paulista, Botucatu, SP, Brasil.
FAU - Peracoli, M T S
AU  - Peracoli MTS
AUID- ORCID: 0000-0002-0936-9512
AD  - Departamento de Ciencias Quimicas e Biologicas, Instituto de Biociencias, 
      Universidade Estadual Paulista, Botucatu, SP, Brasil.
AD  - Departamento de Ginecologia e Obstetricia, Faculdade de Medicina de Botucatu, 
      Universidade Estadual Paulista, Botucatu, SP, Brasil.
LA  - eng
PT  - Journal Article
DEP - 20230109
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de 
      pesquisas medicas e biologicas
JID - 8112917
RN  - 0 (Beclin-1)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.1.1 (MTOR protein, human)
SB  - IM
MH  - Female
MH  - Pregnancy
MH  - Humans
MH  - *Placenta
MH  - Pregnant Women
MH  - *Pre-Eclampsia/genetics
MH  - Beclin-1/genetics/metabolism
MH  - Down-Regulation
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Autophagy/physiology
PMC - PMC9828864
EDAT- 2023/01/12 06:00
MHDA- 2023/01/14 06:00
PMCR- 2023/01/09
CRDT- 2023/01/11 10:05
PHST- 2022/07/27 00:00 [received]
PHST- 2022/11/09 00:00 [accepted]
PHST- 2023/01/11 10:05 [entrez]
PHST- 2023/01/12 06:00 [pubmed]
PHST- 2023/01/14 06:00 [medline]
PHST- 2023/01/09 00:00 [pmc-release]
AID - S0100-879X2022000100683 [pii]
AID - 10.1590/1414-431X2022e12283 [doi]
PST - epublish
SO  - Braz J Med Biol Res. 2023 Jan 9;55:e12283. doi: 10.1590/1414-431X2022e12283. 
      eCollection 2023.