PMID- 36629549
OWN - NLM
STAT- MEDLINE
DCOM- 20230113
LR  - 20230113
IS  - 1678-4375 (Electronic)
IS  - 1519-6984 (Linking)
VI  - 82
DP  - 2023
TI  - Inflammation reduction potential of nanostructured lipid carriers encapsulated 
      with rat's bone marrow cells' lysate.
PG  - e269553
LID - S1519-69842022000100781 [pii]
LID - 10.1590/1519-6984.269553 [doi]
AB  - Bone marrow-derived mesenchymal stromal cells (BMSCs) have been used for treating 
      inflammatory disorders. Due to the large size of BMSCs compared to nanoparticles, 
      BMSCs cannot be loaded into the nanoparticles. It is hypothesized that BMSCs 
      lysate loading into the nanocarriers will effectively deliver cellular contents 
      and regulatory elements of BMSCs at the injury site. This study aimed to 
      investigate nanostructured lipid carriers (NLC) loading with BMSCs lysate through 
      basic characterization and morphological analysis. Moreover, this study was 
      mainly designed to investigate the role of NLC loaded BMSCs lysate in reducing 
      inflammation via in-vitro and in-vivoassays. The in-vitro study involves cell 
      viability assays, p53, annexin V and VEGF expression through ELISA and 
      immunocytochemistry, real-time BAX, caspase-3, IL-6, IL-8, TOP2A, PCNA, and Ki-67 
      gene expression analysis. Additionally, to evaluate in-vivo anti-inflammatory 
      activity, the carrageenan-induced rat paw oedema model was used. In-vitro results 
      showed that NLC loaded BMSCs lysate increased cell viability, decreased apoptosis 
      and pro-inflammatory genes expression and up-regulated angiogenesis and 
      proliferation in H2O2 pre-stimulated cells. Findings of the in-vivo assay also 
      indicated a reduction in rat's paw oedema volume in NLC-loaded BMSCs lysate, and 
      downregulation of BAX, Caspase-3, IL-6, and IL-8 was observed. Enhanced 
      expressions of TOP2A, PCNA, and Ki-67 were obtained. Concluding the results of 
      this study, NLC-loaded BMSCs lysate could reduce inflammation and possibly 
      regenerate damaged tissue mainly via increasing cell viability, angiogenesis and 
      proliferation, and reducing apoptosis and pro-inflammatory cytokines.
FAU - Malik, S
AU  - Malik S
AUID- ORCID: 0000-0002-0709-7226
AD  - The University of Lahore, Institute of Molecular Biology and Biotechnology - 
      IMBB, Lahore, Pakistan.
FAU - Awan, S J
AU  - Awan SJ
AUID- ORCID: 0000-0001-5333-6702
AD  - The University of Lahore, Institute of Molecular Biology and Biotechnology - 
      IMBB, Lahore, Pakistan.
AD  - Kinnaird College For Women, Department of Zoology, Lahore, Pakistan.
FAU - Farzand, A
AU  - Farzand A
AUID- ORCID: 0000-0002-0829-6323
AD  - The University of Lahore, Institute of Molecular Biology and Biotechnology - 
      IMBB, Lahore, Pakistan.
FAU - Ali, Q
AU  - Ali Q
AUID- ORCID: 0000-0002-3160-4830
AD  - University of the Punjab, Department of Plant Breeding and Genetics, Lahore, 
      Pakistan.
LA  - eng
PT  - Journal Article
DEP - 20230106
PL  - Brazil
TA  - Braz J Biol
JT  - Brazilian journal of biology = Revista brasleira de biologia
JID - 101129542
RN  - EC 3.4.22.- (Caspase 3)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Lipids)
SB  - IM
MH  - Rats
MH  - Animals
MH  - Caspase 3/metabolism/pharmacology
MH  - *Hydrogen Peroxide/pharmacology
MH  - bcl-2-Associated X Protein/metabolism/pharmacology
MH  - Proliferating Cell Nuclear Antigen/metabolism/pharmacology
MH  - *Interleukin-6/metabolism/pharmacology
MH  - Interleukin-8/metabolism/pharmacology
MH  - Ki-67 Antigen/metabolism
MH  - Inflammation/chemically induced/metabolism
MH  - Lipids
MH  - Bone Marrow Cells
MH  - Edema/metabolism
EDAT- 2023/01/12 06:00
MHDA- 2023/01/14 06:00
CRDT- 2023/01/11 10:06
PHST- 2022/11/15 00:00 [received]
PHST- 2022/12/01 00:00 [accepted]
PHST- 2023/01/11 10:06 [entrez]
PHST- 2023/01/12 06:00 [pubmed]
PHST- 2023/01/14 06:00 [medline]
AID - S1519-69842022000100781 [pii]
AID - 10.1590/1519-6984.269553 [doi]
PST - epublish
SO  - Braz J Biol. 2023 Jan 6;82:e269553. doi: 10.1590/1519-6984.269553. eCollection 
      2023.