PMID- 36629639 OWN - NLM STAT- MEDLINE DCOM- 20230113 LR - 20230113 IS - 1678-4375 (Electronic) IS - 1519-6984 (Linking) VI - 84 DP - 2023 TI - Antioxidant associated chemoprophylaxis effect of natural spice and green vegetable on hepatotoxicity. PG - e266940 LID - S1519-69842024000100414 [pii] LID - 10.1590/1519-6984.266940 [doi] AB - This is a case control and cohort study, conducted at Department of Biochemistry in FUAST, compressing 24 number of albino Wistar rats. Illicium verum (star anise) natural spice and green vegetable broccoli are used for chemo-preventive herbal treatment in relation with vitamin C antioxidant activity. Hepatotoxicity was induced in liver of model rats by giving interperitoneally single injection of 200 mg/kg bod weight (B.W) of N-nitrosodiethylamine and orally giving 1 mg/kg BW phenobarbital for 14 days during disease prorogation period. The vitamin C analysis from Illicium verum (star anise), green broccoli, orange juice, orange peel and orange pulp was done by iodometric quantitative and qualitative titration method. Rats were given compound according to their bodyweight as 1 mg/kg B.W. After 14 days (Disease Promotion and Propagation Period) and 28 days (Disease cure period) of treatment behavioral activity was monitored by locomotors activity in open field experiment, stimulatory activity in home cage and anxiolytic effects observed in light and dark apparatus and also in T maze. Behavioral activity were significantly increased in Illicium verum and green broccoli treated rats. Hematological study including hemoglobin (Hb), white blood cells (WBC), red blood cells (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red cell distribution width (RDW) and Platelets was done by abbot laboratory analyzer cell. The value of Hb and RBC was also significantly increased in star anise and broccoli treated rats, showed proper function of RBC in microcirculation. WBC was also in normal range revealed that no disease regards to bone marrow and leukemia's. The biochemical activity of rats including urea, creatinine, total protein, albumin, globulin, A/G ratio, amylase and calcium in blood serum were analyzed while, liver health or performance was also determined by LFT (liver function test). Behavioral and laboratorial variables were analyzed by using SPSS v. 20 and p-value < 0.05 was considered statistically significant. The study report noticeable significant effect of Illicium verum and broccoli on hepatotoxicity of experimental model. Recent experimental study statistical outcomes show reciprocal relationship between the consumption of vegetable diet and natural spice with the risk of Hepatotoxicity. Thus, the aim of the study is to enhance the function of star anise or illicium verum natural spice and green vegetable broccoli as an anticancer or chemoprophylaxis agent. FAU - Raza, A A AU - Raza AA AD - Federal Urdu University of Arts Science and Technology, Department of Biochemistry, Karachi, Pakistan. FAU - Mushtaq, R AU - Mushtaq R AD - Federal Urdu University of Arts Science and Technology, Department of Zoology, Karachi, Pakistan. FAU - Khwaja, S AU - Khwaja S AUID- ORCID: 0000-0001-5738-500X AD - Federal Urdu University of Arts Science and Technology, Department of Zoology, Karachi, Pakistan. FAU - Akram, A AU - Akram A AUID- ORCID: 0000-0002-4100-3449 AD - Federal Urdu University of Arts Science and Technology, Department of Zoology, Karachi, Pakistan. FAU - Karim, A AU - Karim A AUID- ORCID: 0000-0002-7041-6023 AD - Sardar Bahadur Khan Women's University Quetta, Department of Zoology, Balochistan, Pakistan. FAU - Akhter, A AU - Akhter A AD - Federal Urdu University of Arts Science and Technology, Department of Zoology, Karachi, Pakistan. LA - eng PT - Journal Article DEP - 20230106 PL - Brazil TA - Braz J Biol JT - Brazilian journal of biology = Revista brasleira de biologia JID - 101129542 RN - 0 (Antioxidants) RN - PQ6CK8PD0R (Ascorbic Acid) SB - IM MH - Rats MH - Animals MH - *Antioxidants/pharmacology/metabolism MH - Vegetables MH - Spices MH - Cohort Studies MH - Rats, Wistar MH - Chemoprevention MH - Ascorbic Acid MH - *Chemical and Drug Induced Liver Injury/prevention & control EDAT- 2023/01/12 06:00 MHDA- 2023/01/14 06:00 CRDT- 2023/01/11 10:07 PHST- 2022/08/16 00:00 [received] PHST- 2022/10/28 00:00 [accepted] PHST- 2023/01/11 10:07 [entrez] PHST- 2023/01/12 06:00 [pubmed] PHST- 2023/01/14 06:00 [medline] AID - S1519-69842024000100414 [pii] AID - 10.1590/1519-6984.266940 [doi] PST - epublish SO - Braz J Biol. 2023 Jan 6;84:e266940. doi: 10.1590/1519-6984.266940. eCollection 2023.