PMID- 36631492
OWN - NLM
STAT- MEDLINE
DCOM- 20230117
LR  - 20230228
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 13
IP  - 1
DP  - 2023 Jan 11
TI  - Accumulation of retrotransposons contributes to W chromosome differentiation in 
      the willow beauty Peribatodes rhomboidaria (Lepidoptera: Geometridae).
PG  - 534
LID - 10.1038/s41598-023-27757-3 [doi]
LID - 534
AB  - The W chromosome of Lepidoptera is typically gene-poor, repeat-rich and composed 
      of heterochromatin. Pioneering studies investigating this chromosome reported an 
      abundance of mobile elements. However, the actual composition of the W chromosome 
      varies greatly between species, as repeatedly demonstrated by comparative genomic 
      hybridization (CGH) or fluorescence in situ hybridization (FISH). Here we present 
      an analysis of repeats on the W chromosome in the willow beauty, Peribatodes 
      rhomboidaria (Geometridae), a species in which CGH predicted an abundance of 
      W-enriched or W-specific sequences. Indeed, comparative analysis of male and 
      female genomes using RepeatExplorer identified ten putative W chromosome-enriched 
      repeats, most of which are LTR or LINE mobile elements. We analysed the two most 
      abundant: PRW LINE-like and PRW Bel-Pao. The results of FISH mapping and 
      bioinformatic analysis confirmed their enrichment on the W chromosome, supporting 
      the hypothesis that mobile elements are the driving force of W chromosome 
      differentiation in Lepidoptera. As the W chromosome is highly underrepresented in 
      chromosome-level genome assemblies of Lepidoptera, this recently introduced 
      approach, combining bioinformatic comparative genome analysis with molecular 
      cytogenetics, provides an elegant tool for studying this elusive and rapidly 
      evolving part of the genome.
CI  - (c) 2023. The Author(s).
FAU - Hejnickova, Martina
AU  - Hejnickova M
AD  - Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech 
      Republic. martinahejnickova@gmail.com.
AD  - Institute of Entomology, Biology Centre CAS, Ceske Budejovice, Czech Republic. 
      martinahejnickova@gmail.com.
FAU - Dalikova, Martina
AU  - Dalikova M
AD  - Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech 
      Republic.
FAU - Zrzava, Magda
AU  - Zrzava M
AD  - Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech 
      Republic.
AD  - Institute of Entomology, Biology Centre CAS, Ceske Budejovice, Czech Republic.
FAU - Marec, Frantisek
AU  - Marec F
AD  - Institute of Entomology, Biology Centre CAS, Ceske Budejovice, Czech Republic.
FAU - Lorite, Pedro
AU  - Lorite P
AD  - Department of Experimental Biology, Genetics Area, University of Jaen, Jaen, 
      Spain.
FAU - Montiel, Eugenia E
AU  - Montiel EE
AD  - Department of Experimental Biology, Genetics Area, University of Jaen, Jaen, 
      Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230111
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Retroelements)
SB  - IM
MH  - Animals
MH  - Female
MH  - Male
MH  - Comparative Genomic Hybridization
MH  - In Situ Hybridization, Fluorescence
MH  - *Moths/genetics
MH  - Retroelements/genetics
MH  - *Salix/genetics
MH  - Sex Chromosomes/genetics
PMC - PMC9834309
COIS- The authors declare no competing interests.
EDAT- 2023/01/12 06:00
MHDA- 2023/01/14 06:00
PMCR- 2023/01/11
CRDT- 2023/01/11 23:15
PHST- 2022/10/12 00:00 [received]
PHST- 2023/01/06 00:00 [accepted]
PHST- 2023/01/11 23:15 [entrez]
PHST- 2023/01/12 06:00 [pubmed]
PHST- 2023/01/14 06:00 [medline]
PHST- 2023/01/11 00:00 [pmc-release]
AID - 10.1038/s41598-023-27757-3 [pii]
AID - 27757 [pii]
AID - 10.1038/s41598-023-27757-3 [doi]
PST - epublish
SO  - Sci Rep. 2023 Jan 11;13(1):534. doi: 10.1038/s41598-023-27757-3.