PMID- 3663478
OWN - NLM
STAT- MEDLINE
DCOM- 19871216
LR  - 20190515
IS  - 0007-0920 (Print)
IS  - 1532-1827 (Electronic)
IS  - 0007-0920 (Linking)
VI  - 56
IP  - 3
DP  - 1987 Sep
TI  - Characterization of radiation sensitivity of human squamous carcinoma A431 cells.
PG  - 301-7
AB  - Studies have been performed to investigate the radiosensitivity of human squamous 
      carcinoma cells. A431 cells were grown in vitro as exponential and fed-plateau 
      monolayer cultures or as multicellular spheroids. Radiobiological studies of 
      various cultures showed that fed-plateau phase cells were more sensitive (D0 = 
      1.3 Gy) than exponentially growing cells (D0 = 1.5 Gy). After a single dose of 12 
      Gy or two doses of 6 Gy irradiation, A431 cultures exhibited a large capacity for 
      potentially lethal damage (PLD) repair (PLD repair factor = 17), but a relatively 
      small sublethal damage (SLD) repair. In order to measure the radiation 
      sensitivity of proliferating (P) and quiescent (Q) cells, enriched populations of 
      P- and Q-cells were isolated from A431 spheroids. Flow cytometric analysis with 
      acridine orange (AO) staining demonstrated that there was a shift of the RNA 
      histograms in fed-plateau and spheroid cultures towards lower values, suggesting 
      the presence of a subpopulation of Q-cells. Centrifugal elutriation was used to 
      isolate the Q-cells from dissociated spheroid cells. Coulter cell volume 
      distributions and flow cytometric analysis showed that Q-cells had a small cell 
      volume (approximately 1380 microns3), low RNA content and a G1-like DNA content. 
      Continuous labelling experiments with tritiated thymidine confirmed the 
      non-proliferating nature of the Q-cells. Irradiation of the Q-cells after 
      isolation from spheroids with between 0 to 10 Gy showed that they were more 
      radiosensitive (decreased D0) than the P-cells isolated from these spheroids. The 
      latter were, however, similar in radiosensitivity to exponential G1 cells.
FAU - Ng, C E
AU  - Ng CE
AD  - Cancer Center, University of Rochester, New York 14642.
FAU - Keng, P C
AU  - Keng PC
FAU - Sutherland, R M
AU  - Sutherland RM
LA  - eng
GR  - CA 11051/CA/NCI NIH HHS/United States
GR  - CA 11198/CA/NCI NIH HHS/United States
GR  - CA 37618/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Br J Cancer
JT  - British journal of cancer
JID - 0370635
RN  - 0 (DNA, Neoplasm)
RN  - 0 (RNA, Neoplasm)
SB  - IM
MH  - Carcinoma, Squamous Cell/*radiotherapy
MH  - Cell Count
MH  - Cell Line
MH  - Cell Separation
MH  - Cell Survival/radiation effects
MH  - DNA, Neoplasm/analysis
MH  - Dose-Response Relationship, Radiation
MH  - Humans
MH  - Mitosis
MH  - RNA, Neoplasm/analysis
MH  - *Radiation Tolerance
PMC - PMC2002214
EDAT- 1987/09/01 00:00
MHDA- 1987/09/01 00:01
CRDT- 1987/09/01 00:00
PHST- 1987/09/01 00:00 [pubmed]
PHST- 1987/09/01 00:01 [medline]
PHST- 1987/09/01 00:00 [entrez]
AID - 10.1038/bjc.1987.193 [doi]
PST - ppublish
SO  - Br J Cancer. 1987 Sep;56(3):301-7. doi: 10.1038/bjc.1987.193.