PMID- 36634885
OWN - NLM
STAT- MEDLINE
DCOM- 20230309
LR  - 20230309
IS  - 1879-3177 (Electronic)
IS  - 0887-2333 (Linking)
VI  - 88
DP  - 2023 Apr
TI  - N-acetyl-L-cysteine attenuated the toxicity of ZIF-8 on EA.hy926 endothelial 
      cells by wnt/beta-catenin pathway.
PG  - 105553
LID - S0887-2333(23)00002-4 [pii]
LID - 10.1016/j.tiv.2023.105553 [doi]
AB  - As kinds of porous crystalline compounds, zeolitic imidazolate frameworks (ZIFs) 
      have been developed quickly and attracted considerable attention for use in nano 
      drug delivery systems, which raised concerns about cardiovascular disorders. At 
      the present, the cytotoxic mechanism of ZIFs in cardiovascular disorders was 
      still unclear. Our experiment explored the toxicity of ZIF-8, a typical kind of 
      ZIFs, on human EA.hy926 vascular endothelial cells. The cell viability, ROS 
      formation, apoptosis level, inflammatory response level, wound healing ability 
      and atherosclerosis-related indicators of EA.hy926 endothelial cells were 
      analyzed after ZIF-8 treatment. Meanwhile, we evaluated the ability of 
      antioxidant N-Acetyl-L-cysteine (NAC) to attenuate the toxicity of ZIF-8 on 
      EA.hy926 endothelial cells. As results, NAC attenuated ROS formation, cell 
      apoptosis, LDH formation and endothelial dysfunction caused by ZIF-8. As the 
      Wnt/beta-catenin pathway was involved in endothelial cell dysfunction, we also 
      studied the expression level of beta-catenin and LEF1 in ZIF-8 and/or NAC treated 
      EA.hy926 cells. As expected, ZIF-8 increased the protein expressions of beta-catenin 
      and LEF1in the IC50 group, which was significantly inhibited by co-treatment with 
      NAC. Taken together, this study could help improve our understanding about the 
      mechanism of ZIF-8-induced endothelial cells injury and NAC had therapeutic 
      potential in preventing ZIF-8-associated endothelial dysfunction by wnt/beta-catenin 
      pathway.
CI  - Copyright (c) 2023. Published by Elsevier Ltd.
FAU - Tang, Yaxin
AU  - Tang Y
AD  - School of Life Science, Shaoxing University, Shaoxing, Zhejiang, China.
FAU - Jin, Lifang
AU  - Jin L
AD  - School of Life Science, Shaoxing University, Shaoxing, Zhejiang, China; Shaoxing 
      Academy of Biomedicine of Zhejiang Sci-Tech University, Shaoxing, Zhejiang, 
      China.
FAU - Qi, Wenwen
AU  - Qi W
AD  - Xiangzhou District People's Hospital, Xiangyang, Hubei, China.
FAU - Gao, Yue
AU  - Gao Y
AD  - School of Life Science, Shaoxing University, Shaoxing, Zhejiang, China.
FAU - Xie, Yixia
AU  - Xie Y
AD  - School of Life Science, Shaoxing University, Shaoxing, Zhejiang, China.
FAU - Xie, Xueying
AU  - Xie X
AD  - School of Life Science, Shaoxing University, Shaoxing, Zhejiang, China.
FAU - Lv, Jianan
AU  - Lv J
AD  - School of Life Science, Shaoxing University, Shaoxing, Zhejiang, China.
FAU - Jiang, Zhikai
AU  - Jiang Z
AD  - The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, 
      Zhejiang, China.
FAU - Jiang, He
AU  - Jiang H
AD  - The First Clinical Medical School of Zhejiang Chinese Medical University, 
      Hangzhou, China.
FAU - Fan, Caixia
AU  - Fan C
AD  - School of Life Science, Shaoxing University, Shaoxing, Zhejiang, China. 
      Electronic address: cxfan2014@sinano.ac.cn.
FAU - Yan, Junyan
AU  - Yan J
AD  - School of Life Science, Shaoxing University, Shaoxing, Zhejiang, China. 
      Electronic address: jyyan2018@usx.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230110
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - WYQ7N0BPYC (Acetylcysteine)
RN  - 0 (beta Catenin)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (acidified zeolitic imidazolate framework-8)
SB  - IM
MH  - Humans
MH  - *Acetylcysteine/pharmacology
MH  - *beta Catenin/metabolism
MH  - *Endothelial Cells/drug effects/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Wnt Signaling Pathway
OTO - NOTNLM
OT  - EA.hy926 endothelial cells
OT  - N-acetyl-L-cysteine (NAC)
OT  - Toxicity
OT  - ZIF-8
COIS- Declaration of Competing Interest The authors have no conflicts of interest to 
      declare.
EDAT- 2023/01/13 06:00
MHDA- 2023/02/25 06:00
CRDT- 2023/01/12 19:22
PHST- 2022/09/13 00:00 [received]
PHST- 2023/01/07 00:00 [revised]
PHST- 2023/01/07 00:00 [accepted]
PHST- 2023/01/13 06:00 [pubmed]
PHST- 2023/02/25 06:00 [medline]
PHST- 2023/01/12 19:22 [entrez]
AID - S0887-2333(23)00002-4 [pii]
AID - 10.1016/j.tiv.2023.105553 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2023 Apr;88:105553. doi: 10.1016/j.tiv.2023.105553. Epub 2023 
      Jan 10.