PMID- 36635574
OWN - NLM
STAT- MEDLINE
DCOM- 20230206
LR  - 20240429
IS  - 2058-5276 (Electronic)
IS  - 2058-5276 (Linking)
VI  - 8
IP  - 2
DP  - 2023 Feb
TI  - Anaerobic methanotroph 'Candidatus Methanoperedens nitroreducens' has a 
      pleomorphic life cycle.
PG  - 321-331
LID - 10.1038/s41564-022-01292-9 [doi]
AB  - 'Candidatus Methanoperedens' are anaerobic methanotrophic (ANME) archaea with 
      global importance to methane cycling. Here meta-omics and fluorescence in situ 
      hybridization (FISH) were applied to characterize a bioreactor dominated by 
      'Candidatus Methanoperedens nitroreducens' performing anaerobic methane oxidation 
      coupled to nitrate reduction. Unexpectedly, FISH revealed the stable co-existence 
      of two 'Ca. M. nitroreducens' morphotypes: the archetypal coccobacilli 
      microcolonies and previously unreported planktonic rods. Metagenomic analysis 
      showed that the 'Ca. M. nitroreducens' morphotypes were genomically identical but 
      had distinct gene expression profiles for proteins associated with carbon 
      metabolism, motility and cell division. In addition, a third distinct phenotype 
      was observed, with some coccobacilli 'Ca. M. nitroreducens' storing carbon as 
      polyhydroxyalkanoates. The phenotypic variation of 'Ca. M. nitroreducens' 
      probably aids their survival and dispersal in the face of sub-optimal 
      environmental conditions. These findings further demonstrate the remarkable 
      ability of members of the 'Ca. Methanoperedens' to adapt to their environment.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer Nature Limited.
FAU - McIlroy, Simon J
AU  - McIlroy SJ
AUID- ORCID: 0000-0003-3749-8730
AD  - Centre for Microbiome Research, School of Biomedical Sciences, Queensland 
      University of Technology (QUT), Translational Research Institute, Woolloongabba, 
      Australia. simon.mcilroy@qut.edu.au.
FAU - Leu, Andy O
AU  - Leu AO
AD  - Centre for Microbiome Research, School of Biomedical Sciences, Queensland 
      University of Technology (QUT), Translational Research Institute, Woolloongabba, 
      Australia.
FAU - Zhang, Xueqin
AU  - Zhang X
AD  - Australian Centre for Water and Environmental Biotechnology (ACWEB), Faculty of 
      Engineering, Architecture and Information Technology, University of Queensland, 
      Brisbane, Australia.
FAU - Newell, Rhys
AU  - Newell R
AUID- ORCID: 0000-0002-1300-6116
AD  - Centre for Microbiome Research, School of Biomedical Sciences, Queensland 
      University of Technology (QUT), Translational Research Institute, Woolloongabba, 
      Australia.
FAU - Woodcroft, Ben J
AU  - Woodcroft BJ
AUID- ORCID: 0000-0003-0670-7480
AD  - Centre for Microbiome Research, School of Biomedical Sciences, Queensland 
      University of Technology (QUT), Translational Research Institute, Woolloongabba, 
      Australia.
FAU - Yuan, Zhiguo
AU  - Yuan Z
AD  - Australian Centre for Water and Environmental Biotechnology (ACWEB), Faculty of 
      Engineering, Architecture and Information Technology, University of Queensland, 
      Brisbane, Australia.
FAU - Hu, Shihu
AU  - Hu S
AUID- ORCID: 0000-0002-1858-8872
AD  - Australian Centre for Water and Environmental Biotechnology (ACWEB), Faculty of 
      Engineering, Architecture and Information Technology, University of Queensland, 
      Brisbane, Australia.
FAU - Tyson, Gene W
AU  - Tyson GW
AUID- ORCID: 0000-0001-8559-9427
AD  - Centre for Microbiome Research, School of Biomedical Sciences, Queensland 
      University of Technology (QUT), Translational Research Institute, Woolloongabba, 
      Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20230112
PL  - England
TA  - Nat Microbiol
JT  - Nature microbiology
JID - 101674869
RN  - OP0UW79H66 (Methane)
SB  - IM
CIN - Nat Microbiol. 2023 Feb;8(2):189-190. PMID: 36635573
MH  - Anaerobiosis
MH  - In Situ Hybridization, Fluorescence
MH  - *Archaea/genetics
MH  - *Bacteria/genetics
MH  - Oxidation-Reduction
MH  - Methanosarcinales/genetics/metabolism
MH  - Methane/metabolism
EDAT- 2023/01/13 06:00
MHDA- 2023/02/07 06:00
CRDT- 2023/01/12 23:27
PHST- 2022/06/24 00:00 [received]
PHST- 2022/11/21 00:00 [accepted]
PHST- 2023/01/13 06:00 [pubmed]
PHST- 2023/02/07 06:00 [medline]
PHST- 2023/01/12 23:27 [entrez]
AID - 10.1038/s41564-022-01292-9 [pii]
AID - 10.1038/s41564-022-01292-9 [doi]
PST - ppublish
SO  - Nat Microbiol. 2023 Feb;8(2):321-331. doi: 10.1038/s41564-022-01292-9. Epub 2023 
      Jan 12.