PMID- 36639049
OWN - NLM
STAT- MEDLINE
DCOM- 20230223
LR  - 20230223
IS  - 1873-6351 (Electronic)
IS  - 0278-6915 (Linking)
VI  - 173
DP  - 2023 Mar
TI  - Luteolin protects against adipogenic and lipogenic potency induced by human 
      relevant mixtures of persistent organic pollutants (POPs) in the 3T3-L1 model.
PG  - 113608
LID - S0278-6915(23)00010-8 [pii]
LID - 10.1016/j.fct.2023.113608 [doi]
AB  - Human exposure to persistent organic pollutants (POPs) may contribute to 
      obesogenic effects. We have previously shown that POP mixtures modelled on blood 
      levels relevant to the Scandinavian population induces adipogenic effects in the 
      mouse 3T3-L1 cell line. Luteolin is a flavone that has shown anti-lipogenic and 
      anti-adipogenic effects on adipogenesis in in vitro models. In this study, 
      luteolin has been applied to inhibit adipocyte formation and intracellular lipid 
      content increase induced by a human relevant mixture of POPs. 3T3-L1 cells were 
      exposed to a POP mixture consisting of 29 chemicals, including amongst others 
      polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs), 
      perfluoroalkylated acids (PFAAs), and polybrominated diphenyl ethers (PBDEs). 
      Rosiglitazone was applied as a positive lipogenic control. Luteolin was tested 
      between 0.5 and 10 muM. High content analysis was used to assess changes in 
      adipocyte formation and intracellular lipid content in the 3T3-L1 cell line. 
      Luteolin significantly reduced POP-induced adipocyte formation at 2, 5 and 10 muM, 
      and lipid accumulation at 10 muM. Interestingly, luteolin did not affect 
      rosiglitazone induced adipo- and lipogenic effects, suggesting differences in 
      mechanisms of action. In conclusion, this in vitro study shows that dietary 
      polyphenols such as luteolin may protect against POP induced adipo- and lipogenic 
      effects.
CI  - Copyright (c) 2023. Published by Elsevier Ltd.
FAU - Xie, Y
AU  - Xie Y
AD  - Institute for Global Food Security, School of Biological Sciences, Queen's 
      University Belfast, Belfast, BT9 5DL, Northern Ireland, United Kingdom.
FAU - Berntsen, H F
AU  - Berntsen HF
AD  - Department of Production Animal Clinical Sciences, Faculty of Veterinary 
      Medicine, Norwegian University of Life Sciences, Post-box 369 sentrum, 0102, 
      Oslo, Norway; National Institute of Occupational Health, P.O. Box 5330, Oslo, 
      Norway.
FAU - Zimmer, K E
AU  - Zimmer KE
AD  - Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, 
      Norwegian University of Life Sciences, Post-box 369 sentrum, 0102, Oslo, Norway.
FAU - Ropstad, E
AU  - Ropstad E
AD  - Department of Production Animal Clinical Sciences, Faculty of Veterinary 
      Medicine, Norwegian University of Life Sciences, Post-box 369 sentrum, 0102, 
      Oslo, Norway.
FAU - Verhaegen, S
AU  - Verhaegen S
AD  - Department of Production Animal Clinical Sciences, Faculty of Veterinary 
      Medicine, Norwegian University of Life Sciences, Post-box 369 sentrum, 0102, 
      Oslo, Norway.
FAU - Connolly, L
AU  - Connolly L
AD  - Institute for Global Food Security, School of Biological Sciences, Queen's 
      University Belfast, Belfast, BT9 5DL, Northern Ireland, United Kingdom. 
      Electronic address: l.connolly@qub.ac.uk.
LA  - eng
PT  - Journal Article
DEP - 20230111
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Persistent Organic Pollutants)
RN  - KUX1ZNC9J2 (Luteolin)
RN  - 05V02F2KDG (Rosiglitazone)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - 0 (Hydrocarbons, Chlorinated)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Pesticides)
RN  - 0 (Lipids)
RN  - 0 (Halogenated Diphenyl Ethers)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Humans
MH  - Adipogenesis
MH  - 3T3-L1 Cells
MH  - Persistent Organic Pollutants
MH  - Luteolin/pharmacology
MH  - Rosiglitazone
MH  - *Polychlorinated Biphenyls/analysis
MH  - *Hydrocarbons, Chlorinated
MH  - *Environmental Pollutants/analysis
MH  - *Pesticides/analysis
MH  - Lipids
MH  - Halogenated Diphenyl Ethers/analysis
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/01/14 06:00
MHDA- 2023/02/25 06:00
CRDT- 2023/01/13 19:27
PHST- 2022/08/29 00:00 [received]
PHST- 2022/12/16 00:00 [revised]
PHST- 2023/01/09 00:00 [accepted]
PHST- 2023/01/14 06:00 [pubmed]
PHST- 2023/02/25 06:00 [medline]
PHST- 2023/01/13 19:27 [entrez]
AID - S0278-6915(23)00010-8 [pii]
AID - 10.1016/j.fct.2023.113608 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2023 Mar;173:113608. doi: 10.1016/j.fct.2023.113608. Epub 2023 
      Jan 11.