PMID- 36639644
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230116
IS  - 2049-3002 (Print)
IS  - 2049-3002 (Electronic)
IS  - 2049-3002 (Linking)
VI  - 11
IP  - 1
DP  - 2023 Jan 13
TI  - Arginase-1 inhibition reduces migration ability and metastatic colonization of 
      colon cancer cells.
PG  - 1
LID - 10.1186/s40170-022-00301-z [doi]
LID - 1
AB  - BACKGROUND: Arginase-1 (ARG1), a urea cycle-related enzyme, catalyzes the 
      hydrolysis of arginine to urea and ornithine, which regulates the proliferation, 
      differentiation, and function of various cells. However, it is unclear whether 
      ARG1 controls the progression and malignant alterations of colon cancer. METHODS: 
      We established metastatic colonization mouse model and ARG1 overexpressing murine 
      colon cancer CT26 cells to investigate whether activation of ARG1 was related to 
      malignancy of colon cancer cells in vivo. Living cell numbers and migration 
      ability of CT26 cells were evaluated in the presence of ARG inhibitor in vitro. 
      RESULTS: Inhibition of arginase activity significantly suppressed the 
      proliferation and migration ability of CT26 murine colon cancer cells in vitro. 
      Overexpression of ARG1 in CT26 cells reduced intracellular L-arginine levels, 
      enhanced cell migration, and promoted epithelial-mesenchymal transition. 
      Metastatic colonization of CT26 cells in lung and liver tissues was significantly 
      augmented by ARG1 overexpression in vivo. ARG1 gene expression was higher in the 
      tumor tissues of liver metastasis than those of primary tumor, and arginase 
      inhibition suppressed the migration ability of HCT116 human colon cancer cells. 
      CONCLUSION: Activation of ARG1 is related to the migration ability and metastatic 
      colonization of colon cancer cells, and blockade of this process may be a novel 
      strategy for controlling cancer malignancy.
CI  - (c) 2022. The Author(s).
FAU - Wang, Xiangdong
AU  - Wang X
AD  - Division of Functional Immunology, Section of Disease Control, Institute for 
      Genetic Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, 
      060-0815, Japan.
FAU - Xiang, Huihui
AU  - Xiang H
AD  - Division of Functional Immunology, Section of Disease Control, Institute for 
      Genetic Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, 
      060-0815, Japan.
AD  - Molecular Pathology and Genetics Division, Kanagawa Cancer Center Research 
      Institute, Yokohama, 241-8515, Japan.
FAU - Toyoshima, Yujiro
AU  - Toyoshima Y
AD  - Department of Gastroenterological Surgery I, Hokkaido University Graduate School 
      of Medicine, Sapporo, 060-8638, Japan.
FAU - Shen, Weidong
AU  - Shen W
AD  - Division of Functional Immunology, Section of Disease Control, Institute for 
      Genetic Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, 
      060-0815, Japan.
FAU - Shichi, Shunsuke
AU  - Shichi S
AD  - Division of Functional Immunology, Section of Disease Control, Institute for 
      Genetic Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, 
      060-0815, Japan.
AD  - Department of Gastroenterological Surgery I, Hokkaido University Graduate School 
      of Medicine, Sapporo, 060-8638, Japan.
FAU - Nakamoto, Hiroki
AU  - Nakamoto H
AD  - Division of Functional Immunology, Section of Disease Control, Institute for 
      Genetic Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, 
      060-0815, Japan.
AD  - Department of Gastroenterological Surgery I, Hokkaido University Graduate School 
      of Medicine, Sapporo, 060-8638, Japan.
FAU - Kimura, Saori
AU  - Kimura S
AD  - Division of Functional Immunology, Section of Disease Control, Institute for 
      Genetic Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, 
      060-0815, Japan.
AD  - Department of Gastroenterological Surgery I, Hokkaido University Graduate School 
      of Medicine, Sapporo, 060-8638, Japan.
FAU - Sugiyama, Ko
AU  - Sugiyama K
AD  - Division of Functional Immunology, Section of Disease Control, Institute for 
      Genetic Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, 
      060-0815, Japan.
AD  - Department of Gastroenterological Surgery I, Hokkaido University Graduate School 
      of Medicine, Sapporo, 060-8638, Japan.
FAU - Homma, Shigenori
AU  - Homma S
AD  - Department of Gastroenterological Surgery I, Hokkaido University Graduate School 
      of Medicine, Sapporo, 060-8638, Japan.
FAU - Miyagi, Yohei
AU  - Miyagi Y
AD  - Molecular Pathology and Genetics Division, Kanagawa Cancer Center Research 
      Institute, Yokohama, 241-8515, Japan.
FAU - Taketomi, Akinobu
AU  - Taketomi A
AD  - Department of Gastroenterological Surgery I, Hokkaido University Graduate School 
      of Medicine, Sapporo, 060-8638, Japan.
FAU - Kitamura, Hidemitsu
AU  - Kitamura H
AD  - Division of Functional Immunology, Section of Disease Control, Institute for 
      Genetic Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, 
      060-0815, Japan. kitamura@igm.hokudai.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20230113
PL  - England
TA  - Cancer Metab
JT  - Cancer & metabolism
JID - 101607582
PMC - PMC9838026
OTO - NOTNLM
OT  - Arginase 1
OT  - Arginine
OT  - Colorectal cancer
OT  - Malignancy
OT  - Metastatic colonization
COIS- The authors declare that they have no competing interests.
EDAT- 2023/01/14 06:00
MHDA- 2023/01/14 06:01
PMCR- 2023/01/13
CRDT- 2023/01/13 23:30
PHST- 2022/05/20 00:00 [received]
PHST- 2022/12/14 00:00 [accepted]
PHST- 2023/01/13 23:30 [entrez]
PHST- 2023/01/14 06:00 [pubmed]
PHST- 2023/01/14 06:01 [medline]
PHST- 2023/01/13 00:00 [pmc-release]
AID - 10.1186/s40170-022-00301-z [pii]
AID - 301 [pii]
AID - 10.1186/s40170-022-00301-z [doi]
PST - epublish
SO  - Cancer Metab. 2023 Jan 13;11(1):1. doi: 10.1186/s40170-022-00301-z.