PMID- 36639763 OWN - NLM STAT- MEDLINE DCOM- 20230117 LR - 20230117 IS - 1477-7827 (Electronic) IS - 1477-7827 (Linking) VI - 21 IP - 1 DP - 2023 Jan 13 TI - Features of peritoneal dendritic cells in the development of endometriosis. PG - 4 LID - 10.1186/s12958-023-01058-w [doi] LID - 4 AB - BACKGROUND: Emerging evidence of immunological dysfunction have been described in endometriosis. Dendritic cells (DCs), one of the main antigen-presenting cells, are specialized in the initiation and modulation of the adaptive immune response. Emerging studies demonstrated both endometrial and circulating differences in DCs populations in women with endometriosis. However, the role and mechanism of peritoneal DCs in endometriosis is still unclear. The present study was undertaken to explore the features of peritoneal DCs in the pathogenesis of endometriosis. This study is beneficial to further clarify the cause of endometriosis and provide a new insight into the medical treatment for endometriosis. METHODS: The study included 12 women with endometriosis and 11 women without endometriosis. The C57BL6 mouse model of endometriosis was established by intraperitoneal injection of endometrial segments. The peritoneal DCs of endometriosis patients and mouse models were analyzed by fluorescence associated cell sorting (FACS) examination. RESULTS: Increased cell density of peritoneal DCs were observed in endometriosis patients. Moreover, the proportion of mature DCs (mDCs, CD80(high)CD1a(low) cells) in the peritoneal DCs was lower whereas the proportion of immature DCs (iDCs, CD80(low)CD1a(high) cells) was increased in endometriosis patients. Similarly, the cell density of peritoneal DCs in murine models increased immediately after the injection of endometrial tissues and reached the highest level at 14 days. In addition, the proportion of mDCs (CD11c(high)CD80(high) cells) in the peritoneal DCs decreased immediately after the injection of endometrial tissues and then increased with the time until 42 days, but still lower than the control group. In contrast, the proportion of iDCs (CD11c(high)CD80(low) cells) in the peritoneal DCs showed the opposite dynamic changes. However, after treated with LPS, the mDCs proportion was significantly increased, leading to lower volume and weight of the endometriosis lesions. CONCLUSIONS: Increased level of peritoneal DCs facilitated the pathogenesis of endometriosis lesions, especially in the early stage of the disease. Furthermore, peritoneal DCs maturation played an important role in the development of endometriosis. CI - (c) 2023. The Author(s). FAU - Qiaomei, Zheng AU - Qiaomei Z AD - Department of Obstetrics and Gynecology, Fujian Key Laboratory of Precision Medicine for Cancer, the First Affiliated Hospital, Fujian Medical University, 20 Chazhong Road, Fuzhou, Fujian, 350005, People's Republic of China. AD - Department of Gynecology, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, 999 Huashan Road, Fuzhou, Fujian, 350212, People's Republic of China. FAU - Ping, Wu AU - Ping W AD - Department of Gynecology, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, 999 Huashan Road, Fuzhou, Fujian, 350212, People's Republic of China. AD - Department of Pathology, the First Affiliated Hospital, Fujian Medical University, 20 Chazhong Road, Fuzhou, Fujian, 350005, People's Republic of China. FAU - Yanjing, Zhao AU - Yanjing Z AD - Department of Surgery, 92403 Military Hospital, Fuzhou, Fujian, 350015, People's Republic of China. FAU - Jinhua, Wang AU - Jinhua W AD - Department of Obstetrics and Gynecology, Fujian Key Laboratory of Precision Medicine for Cancer, the First Affiliated Hospital, Fujian Medical University, 20 Chazhong Road, Fuzhou, Fujian, 350005, People's Republic of China. AD - Department of Gynecology, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, 999 Huashan Road, Fuzhou, Fujian, 350212, People's Republic of China. FAU - Shaozhan, Chen AU - Shaozhan C AD - Department of Obstetrics and Gynecology, Fujian Key Laboratory of Precision Medicine for Cancer, the First Affiliated Hospital, Fujian Medical University, 20 Chazhong Road, Fuzhou, Fujian, 350005, People's Republic of China. AD - Department of Gynecology, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, 999 Huashan Road, Fuzhou, Fujian, 350212, People's Republic of China. FAU - Lihong, Chen AU - Lihong C AD - Department of Obstetrics and Gynecology, Fujian Key Laboratory of Precision Medicine for Cancer, the First Affiliated Hospital, Fujian Medical University, 20 Chazhong Road, Fuzhou, Fujian, 350005, People's Republic of China. chenlihong_0102@126.com. AD - Department of Gynecology, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, 999 Huashan Road, Fuzhou, Fujian, 350212, People's Republic of China. chenlihong_0102@126.com. LA - eng GR - 2018Y9077/Joint Funds for the Innovation of Science and Technology, Fujian Province/ GR - 2020GGB031/Fujian Provincial Health Technology Project/ GR - 2022J01221/Natural Science Fundation of Fujian Province/ PT - Journal Article DEP - 20230113 PL - England TA - Reprod Biol Endocrinol JT - Reproductive biology and endocrinology : RB&E JID - 101153627 SB - IM MH - Humans MH - Female MH - Animals MH - Mice MH - *Endometriosis/pathology MH - Cell Differentiation MH - Endometrium/pathology MH - Dendritic Cells PMC - PMC9837895 OTO - NOTNLM OT - DCs maturation OT - Endometriosis OT - Immature DCs OT - Mature DCs OT - Peritoneal dendritic cells COIS- The authors declare that they have no competing interests. EDAT- 2023/01/14 06:00 MHDA- 2023/01/18 06:00 PMCR- 2023/01/13 CRDT- 2023/01/13 23:39 PHST- 2022/10/28 00:00 [received] PHST- 2023/01/10 00:00 [accepted] PHST- 2023/01/13 23:39 [entrez] PHST- 2023/01/14 06:00 [pubmed] PHST- 2023/01/18 06:00 [medline] PHST- 2023/01/13 00:00 [pmc-release] AID - 10.1186/s12958-023-01058-w [pii] AID - 1058 [pii] AID - 10.1186/s12958-023-01058-w [doi] PST - epublish SO - Reprod Biol Endocrinol. 2023 Jan 13;21(1):4. doi: 10.1186/s12958-023-01058-w.