PMID- 36642356 OWN - NLM STAT- MEDLINE DCOM- 20230224 LR - 20230224 IS - 1879-0003 (Electronic) IS - 0141-8130 (Linking) VI - 231 DP - 2023 Mar 15 TI - Synthesis, modification and application of fish skin gelatin-based hydrogel as sustainable and versatile bioresource of antidiabetic peptide. PG - 123248 LID - S0141-8130(23)00124-1 [pii] LID - 10.1016/j.ijbiomac.2023.123248 [doi] AB - Gelatin hydrogel is widely employed in various fields, however, commercially available gelatin hydrogels are mostly derived from mammalian which has many disadvantages due to the supply and ethical issues. In this study, the properties of hydrogels from fish-derived collagen fabricated with varying Glutaraldehyde (GA) determined. The antidiabetic properties of salmon gelatin (SG) and tilapia gelatin (TG) was also evaluated against alpha-glucosidase. Glutaraldehyde-crosslinked salmon gelatin and tilapia gelatin were used, and compared with different concentrations of GA by 0.05 %, 0.1 %, and 0.15 %. Water absorbency, swelling, porosity, pore size and water retention of the hydrogels were dependent on the degree of crosslinking. The synthesis of hydrogels was confirmed by FTIR study. Scanning electron microscope (SEM) observation showed that all hydrogels have a porous structure with irregular shapes and heterogeneous morphology. Performance tests showed that gelatin-GA 0.05 % mixture had the best performance. Antidiabetic bioactivity in vitro and in silico tests showed that the active peptides of SG and TG showed a high binding affinity to alpha-glucosidase enzyme. In conclusion, SG and TG cross-linked GA 0.05 % have the potential as an antidiabetic agent and as a useful option over mammalian-derived gelatin. CI - Copyright (c) 2023 Elsevier B.V. All rights reserved. FAU - Munawaroh, Heli Siti Halimatul AU - Munawaroh HSH AD - Study Program of Chemistry, Department of Chemistry Education, Universitas Pendidikan Indonesia, Jalan Dr. Setiabudhi 229, Bandung 40154, Indonesia. Electronic address: heli@upi.edu. FAU - Pratiwi, Riska Nur AU - Pratiwi RN AD - Study Program of Chemistry, Department of Chemistry Education, Universitas Pendidikan Indonesia, Jalan Dr. Setiabudhi 229, Bandung 40154, Indonesia. FAU - Gumilar, Gun Gun AU - Gumilar GG AD - Study Program of Chemistry, Department of Chemistry Education, Universitas Pendidikan Indonesia, Jalan Dr. Setiabudhi 229, Bandung 40154, Indonesia. FAU - Aisyah, Siti AU - Aisyah S AD - Study Program of Chemistry, Department of Chemistry Education, Universitas Pendidikan Indonesia, Jalan Dr. Setiabudhi 229, Bandung 40154, Indonesia. FAU - Rohilah, Siti AU - Rohilah S AD - Study Program of Chemistry, Department of Chemistry Education, Universitas Pendidikan Indonesia, Jalan Dr. Setiabudhi 229, Bandung 40154, Indonesia. FAU - Nurjanah, Anisa AU - Nurjanah A AD - Study Program of Chemistry, Department of Chemistry Education, Universitas Pendidikan Indonesia, Jalan Dr. Setiabudhi 229, Bandung 40154, Indonesia. FAU - Ningrum, Andriati AU - Ningrum A AD - Department of Food Science and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta 5528, Indonesia. FAU - Susanto, Eko AU - Susanto E AD - Faculty of Fisheries and Marine Science, Universitas Diponegoro, Jalan Prof. Jacub Rais Tembalang, Semarang 50275, Indonesia. FAU - Pratiwi, Amelinda AU - Pratiwi A AD - Study Program of Chemistry, Department of Chemistry Education, Universitas Pendidikan Indonesia, Jalan Dr. Setiabudhi 229, Bandung 40154, Indonesia. FAU - Arindita, Ni Putu Yunika AU - Arindita NPY AD - Study Program of Chemistry, Department of Chemistry Education, Universitas Pendidikan Indonesia, Jalan Dr. Setiabudhi 229, Bandung 40154, Indonesia. FAU - Martha, Larasati AU - Martha L AD - Laboratory of Biopharmaceutics, Department of Pharmacology, Faculty of Pharmacy, Takasaki University of Health and Welfare, 60 Nakaorui-machi, Takasaki City, Gunma prefecture 370-0033, Japan. FAU - Chew, Kit Wayne AU - Chew KW AD - School of Chemistry, Chemical Engineering and Biotechnology, Nanyang Technological University, 62 Nanyang Drive, Singapore 637459, Singapore. FAU - Show, Pau-Loke AU - Show PL AD - Zhejiang Provincial Key Laboratory for Subtropical Water Environment and Marine Biological Resources Protection, Wenzhou University, Wenzhou 325035, China; Department of Chemical Engineering, Khalifa University, Shakhbout Bin Sultan St - Zone 1 - Abu Dhabi - United Arab Emirates; Department of Sustainable Engineering, Saveetha School of Engineering, SIMATS, Chennai 602105, India; Department of Chemical and Environmental Engineering, University of Nottingham Malaysia, Jalan Broga 43500, Selangor, Malaysia. Electronic address: Pauloke.Show@nottingham.edu.my. LA - eng PT - Journal Article DEP - 20230112 PL - Netherlands TA - Int J Biol Macromol JT - International journal of biological macromolecules JID - 7909578 RN - 0 (Hydrogels) RN - 9000-70-8 (Gelatin) RN - T3C89M417N (Glutaral) RN - EC 3.2.1.20 (alpha-Glucosidases) RN - 0 (Peptides) RN - 059QF0KO0R (Water) SB - IM MH - Animals MH - *Hydrogels/chemistry MH - *Gelatin/chemistry MH - Glutaral MH - alpha-Glucosidases MH - Peptides MH - Water/chemistry MH - Mammals OTO - NOTNLM OT - Antidiabetic OT - Crosslinking OT - Fish skin OT - Gelatin hydrogel OT - Glutaraldehyde COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2023/01/16 06:00 MHDA- 2023/02/25 06:00 CRDT- 2023/01/15 19:27 PHST- 2022/10/21 00:00 [received] PHST- 2022/12/24 00:00 [revised] PHST- 2023/01/09 00:00 [accepted] PHST- 2023/01/16 06:00 [pubmed] PHST- 2023/02/25 06:00 [medline] PHST- 2023/01/15 19:27 [entrez] AID - S0141-8130(23)00124-1 [pii] AID - 10.1016/j.ijbiomac.2023.123248 [doi] PST - ppublish SO - Int J Biol Macromol. 2023 Mar 15;231:123248. doi: 10.1016/j.ijbiomac.2023.123248. Epub 2023 Jan 12.