PMID- 36649130
OWN - NLM
STAT- MEDLINE
DCOM- 20230222
LR  - 20230226
IS  - 1554-8937 (Electronic)
IS  - 1554-8929 (Print)
IS  - 1554-8929 (Linking)
VI  - 18
IP  - 2
DP  - 2023 Feb 17
TI  - Profiling Sulfur(VI) Fluorides as Reactive Functionalities for Chemical Biology 
      Tools and Expansion of the Ligandable Proteome.
PG  - 285-295
LID - 10.1021/acschembio.2c00633 [doi]
AB  - Here, we report a comprehensive profiling of sulfur(VI) fluorides (S(VI)-Fs) as 
      reactive groups for chemical biology applications. S(VI)-Fs are reactive 
      functionalities that modify lysine, tyrosine, histidine, and serine sidechains. A 
      panel of S(VI)-Fs were studied with respect to hydrolytic stability and 
      reactivity with nucleophilic amino acid sidechains. The use of S(VI)-Fs to 
      covalently modify carbonic anhydrase II (CAII) and a range of kinases was then 
      investigated. Finally, the S(VI)-F panel was used in live cell chemoproteomic 
      workflows, identifying novel protein targets based on the type of S(VI)-F used. 
      This work highlights how S(VI)-F reactivity can be used as a tool to expand the 
      liganded proteome.
FAU - Gilbert, Katharine E
AU  - Gilbert KE
AD  - GlaxoSmithKline, Gunnels Wood Road, Stevenage, HertfordshireSG1 2NY, United 
      Kingdom.
AD  - University of Strathclyde, 295 Cathedral Street, GlasgowG11XL, United Kingdom.
FAU - Vuorinen, Aini
AU  - Vuorinen A
AD  - Crick-GSK Biomedical LinkLabs, GlaxoSmithKline, Gunnels Wood Road, StevenageSG1 
      2NY, United Kingdom.
FAU - Aatkar, Arron
AU  - Aatkar A
AD  - GlaxoSmithKline, Gunnels Wood Road, Stevenage, HertfordshireSG1 2NY, United 
      Kingdom.
AD  - University of Strathclyde, 295 Cathedral Street, GlasgowG11XL, United Kingdom.
FAU - Pogany, Peter
AU  - Pogany P
AD  - GlaxoSmithKline, Gunnels Wood Road, Stevenage, HertfordshireSG1 2NY, United 
      Kingdom.
FAU - Pettinger, Jonathan
AU  - Pettinger J
AD  - Crick-GSK Biomedical LinkLabs, GlaxoSmithKline, Gunnels Wood Road, StevenageSG1 
      2NY, United Kingdom.
FAU - Grant, Emma K
AU  - Grant EK
AD  - GlaxoSmithKline, Gunnels Wood Road, Stevenage, HertfordshireSG1 2NY, United 
      Kingdom.
FAU - Kirkpatrick, Joanna M
AU  - Kirkpatrick JM
AUID- ORCID: 0000-0001-9291-7294
AD  - The Francis Crick Institute, 1 Midland Road, LondonNW1 1AT, United Kingdom.
FAU - Rittinger, Katrin
AU  - Rittinger K
AUID- ORCID: 0000-0002-7698-4435
AD  - The Francis Crick Institute, 1 Midland Road, LondonNW1 1AT, United Kingdom.
FAU - House, David
AU  - House D
AUID- ORCID: 0000-0002-9635-2451
AD  - GlaxoSmithKline, Gunnels Wood Road, Stevenage, HertfordshireSG1 2NY, United 
      Kingdom.
AD  - Crick-GSK Biomedical LinkLabs, GlaxoSmithKline, Gunnels Wood Road, StevenageSG1 
      2NY, United Kingdom.
FAU - Burley, Glenn A
AU  - Burley GA
AUID- ORCID: 0000-0002-4896-113X
AD  - University of Strathclyde, 295 Cathedral Street, GlasgowG11XL, United Kingdom.
FAU - Bush, Jacob T
AU  - Bush JT
AUID- ORCID: 0000-0001-7165-0092
AD  - GlaxoSmithKline, Gunnels Wood Road, Stevenage, HertfordshireSG1 2NY, United 
      Kingdom.
AD  - Crick-GSK Biomedical LinkLabs, GlaxoSmithKline, Gunnels Wood Road, StevenageSG1 
      2NY, United Kingdom.
LA  - eng
GR  - Medical Research Council/United Kingdom
GR  - Cancer Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230117
PL  - United States
TA  - ACS Chem Biol
JT  - ACS chemical biology
JID - 101282906
RN  - 0 (Proteome)
RN  - Q80VPU408O (Fluorides)
RN  - 70FD1KFU70 (Sulfur)
RN  - 0 (Amino Acids)
SB  - IM
MH  - *Proteome/metabolism
MH  - *Fluorides/chemistry
MH  - Sulfur/chemistry
MH  - Amino Acids/chemistry
MH  - Biology
PMC - PMC9942091
COIS- The authors declare no competing financial interest.
EDAT- 2023/01/18 06:00
MHDA- 2023/02/22 06:00
PMCR- 2023/02/21
CRDT- 2023/01/17 12:23
PHST- 2023/01/18 06:00 [pubmed]
PHST- 2023/02/22 06:00 [medline]
PHST- 2023/01/17 12:23 [entrez]
PHST- 2023/02/21 00:00 [pmc-release]
AID - 10.1021/acschembio.2c00633 [doi]
PST - ppublish
SO  - ACS Chem Biol. 2023 Feb 17;18(2):285-295. doi: 10.1021/acschembio.2c00633. Epub 
      2023 Jan 17.