PMID- 36652270
OWN - NLM
STAT- MEDLINE
DCOM- 20230509
LR  - 20230509
IS  - 1942-7611 (Electronic)
IS  - 1942-7603 (Linking)
VI  - 15
IP  - 5
DP  - 2023 May
TI  - Development of mass spectrometry-based methods for the detection of 
      11-ketotestosterone and 11-ketodihydrotestosterone.
PG  - 566-578
LID - 10.1002/dta.3442 [doi]
AB  - The anabolic properties of 11-hydroxyandrostenedione (OHA4) and its 
      physiologically active metabolites 11-ketotestosterone (KT) and 
      11-ketodihydrotestosterone (KDHT) have been discussed in several recent 
      publications. Especially KT has become readily available via internet-based 
      providers. No doping control methods for the detection of KT or KDHT exist, 
      neither on the initial testing procedure level nor as confirmatory assay. Probing 
      for the misuse of adrenosterone, the prohormone of OHA4, has already been 
      addressed, and the suggested marker for its misuse was mainly the urinary 
      concentration of 11-hydroxyandrosterone (OHA). In addition, for confirmation 
      purposes, the carbon isotope ratios (CIR) were taken into consideration. The 
      urinary concentration of OHA is highly variable, and the endogenous dilution 
      after exogenous administration may therefore be considerable; hence, described 
      approaches resulted in short detection times. In this study, the human metabolism 
      of KT was investigated in order to provide additional means for the detection of 
      KT and its prohormone OHA4. Two volunteers (one female and one male) orally 
      administered 20 mg of KT each, and urine samples were collected for 5 days. 
      Urinary concentrations of KT and its metabolites were investigated, and a 
      reference population encompassing 220 male and female athletes was investigated 
      in order to elucidate preliminary thresholds. As confirmation procedure, an 
      isotope ratio mass spectrometry-based method was developed in order to determine 
      the CIR of KT and relevant metabolites. The developed methods enabled the 
      detection of exogenous KT for more than 20 h after a single oral administration, 
      which is comparable to a single oral testosterone administration.
CI  - (c) 2023 The Authors. Drug Testing and Analysis published by John Wiley & Sons Ltd.
FAU - Piper, Thomas
AU  - Piper T
AUID- ORCID: 0000-0002-7462-6693
AD  - Center for Preventive Doping Research - Institute of Biochemistry, German Sport 
      University Cologne, Koln, Germany.
FAU - Thevis, Mario
AU  - Thevis M
AUID- ORCID: 0000-0002-1535-6451
AD  - Center for Preventive Doping Research - Institute of Biochemistry, German Sport 
      University Cologne, Koln, Germany.
AD  - European Monitoring Center for Emerging Doping Agents (EuMoCEDA), Bonn, Germany.
LA  - eng
GR  - 20A13MT/World Anti-Doping Agency/
GR  - Manfred-Donike Institute for Doping Analysis/
GR  - Federal Ministry of the Interior, Building and Community/
PT  - Journal Article
DEP - 20230203
PL  - England
TA  - Drug Test Anal
JT  - Drug testing and analysis
JID - 101483449
RN  - KF38W1A85U (11-ketotestosterone)
RN  - 0 (11-ketodihydrotestosterone)
RN  - 3XMK78S47O (Testosterone)
RN  - 0 (Carbon Isotopes)
SB  - IM
MH  - Humans
MH  - Male
MH  - Female
MH  - Gas Chromatography-Mass Spectrometry/methods
MH  - Mass Spectrometry
MH  - *Testosterone/metabolism
MH  - Carbon Isotopes/analysis
MH  - *Doping in Sports
MH  - Substance Abuse Detection/methods
OTO - NOTNLM
OT  - 11-ketotestosterone
OT  - adrenosterone
OT  - carbon isotope ratios
OT  - doping controls
OT  - isotope ratio mass spectrometry
EDAT- 2023/01/19 06:00
MHDA- 2023/05/09 06:42
CRDT- 2023/01/18 11:34
PHST- 2023/01/16 00:00 [revised]
PHST- 2022/12/20 00:00 [received]
PHST- 2023/01/16 00:00 [accepted]
PHST- 2023/05/09 06:42 [medline]
PHST- 2023/01/19 06:00 [pubmed]
PHST- 2023/01/18 11:34 [entrez]
AID - 10.1002/dta.3442 [doi]
PST - ppublish
SO  - Drug Test Anal. 2023 May;15(5):566-578. doi: 10.1002/dta.3442. Epub 2023 Feb 3.