PMID- 36653166
OWN - NLM
STAT- MEDLINE
DCOM- 20230202
LR  - 20230202
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 71
IP  - 4
DP  - 2023 Feb 1
TI  - Genistein Protects against Acetaldehyde-Induced Oxidative Stress and Hepatocyte 
      Injury in Chronic Alcohol-Fed Mice.
PG  - 1930-1943
LID - 10.1021/acs.jafc.2c05747 [doi]
AB  - Alcohol-related liver disease (ALD) is one of the most prevalent forms of liver 
      disease in the world. Acetaldehyde, an intermediate product of alcohol 
      catabolism, is a cause of liver injury caused by alcohol. This study was designed 
      to evaluate the protective role and mechanism(s) of genistein against 
      acetaldehyde-induced liver injury in the pathological process of ALD. We found 
      that genistein administration significantly ameliorated alcohol-induced hepatic 
      steatosis, injury, and inflammation in mice. Genistein supplementation markedly 
      reversed hepatic oxidative stress, endoplasmic reticulum stress, mitochondrial 
      dysfunction, and hepatocellular apoptosis in both alcohol-fed mice liver and 
      acetaldehyde-treated hepatocytes. The mechanistic experiments revealed that the 
      restoration of genistein administration rescued heme oxygenase-1 (HO-1) reduction 
      at both transcriptional and protein levels in either alcohol-fed mice liver or 
      acetaldehyde-treated hepatocytes, and the beneficial aspects derived from 
      genistein were abolished in antioxidase heme oxygenase-1 (HO-1)-deficient 
      hepatocytes. Moreover, we confirmed that genistein administration-restored 
      hepatic nuclear factor erythroid 2-related factor 2 (NRF2), a key transcriptional 
      regulator of HO-1, was involved in the protective role of genistein in ALD. This 
      study demonstrated that genistein ameliorated acetaldehyde-induced oxidative 
      stress and liver injury by restoring the hepatic NRF2-HO-1 signaling pathway in 
      response to chronic alcohol consumption. Therefore, genistein may serve as a 
      potential therapeutic choice for the treatment of ALD.
FAU - Ding, Qinchao
AU  - Ding Q
AUID- ORCID: 0000-0001-8664-5368
AD  - School of Public Health, Zhejiang Chinese Medical University, Hangzhou 310053, 
      Zhejiang, P. R. China.
AD  - College of Animal Science, Zhejiang University, Hangzhou 310058, Zhejiang, P. R. 
      China.
FAU - Pi, Aiwen
AU  - Pi A
AD  - School of Public Health, Zhejiang Chinese Medical University, Hangzhou 310053, 
      Zhejiang, P. R. China.
FAU - Hao, Liuyi
AU  - Hao L
AD  - School of Public Health, Zhejiang Chinese Medical University, Hangzhou 310053, 
      Zhejiang, P. R. China.
FAU - Xu, Tiantian
AU  - Xu T
AD  - School of Public Health, Zhejiang Chinese Medical University, Hangzhou 310053, 
      Zhejiang, P. R. China.
FAU - Zhu, Qin
AU  - Zhu Q
AD  - Department of Clinical Nutrition, Affiliated Zhejiang Hospital, School of 
      Medicine, Zhejiang University, Hangzhou 310013, Zhejiang, P. R. China.
FAU - Shu, Long
AU  - Shu L
AD  - Department of Clinical Nutrition, Affiliated Zhejiang Hospital, School of 
      Medicine, Zhejiang University, Hangzhou 310013, Zhejiang, P. R. China.
FAU - Yu, Xiaolong
AU  - Yu X
AD  - Department of Clinical Nutrition, Affiliated Zhejiang Hospital, School of 
      Medicine, Zhejiang University, Hangzhou 310013, Zhejiang, P. R. China.
FAU - Wang, Weiguang
AU  - Wang W
AD  - Department of Clinical Nutrition, Affiliated Zhejiang Hospital, School of 
      Medicine, Zhejiang University, Hangzhou 310013, Zhejiang, P. R. China.
FAU - Si, Caijuan
AU  - Si C
AD  - Department of Clinical Nutrition, Affiliated Zhejiang Hospital, School of 
      Medicine, Zhejiang University, Hangzhou 310013, Zhejiang, P. R. China.
FAU - Li, Songtao
AU  - Li S
AUID- ORCID: 0000-0002-2926-673X
AD  - School of Public Health, Zhejiang Chinese Medical University, Hangzhou 310053, 
      Zhejiang, P. R. China.
AD  - Department of Clinical Nutrition, Affiliated Zhejiang Hospital, School of 
      Medicine, Zhejiang University, Hangzhou 310013, Zhejiang, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20230118
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - GO1N1ZPR3B (Acetaldehyde)
RN  - DH2M523P0H (Genistein)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Acetaldehyde/metabolism
MH  - *Genistein/metabolism
MH  - Heme Oxygenase-1/genetics/metabolism
MH  - NF-E2-Related Factor 2/genetics/metabolism
MH  - Ethanol/metabolism
MH  - Hepatocytes/metabolism
MH  - Liver/metabolism
MH  - Oxidative Stress
OTO - NOTNLM
OT  - acetaldehyde
OT  - alcohol-related liver disease
OT  - genistein
OT  - inflammation
OT  - oxidative stress
EDAT- 2023/01/19 06:00
MHDA- 2023/02/03 06:00
CRDT- 2023/01/18 21:22
PHST- 2023/01/19 06:00 [pubmed]
PHST- 2023/02/03 06:00 [medline]
PHST- 2023/01/18 21:22 [entrez]
AID - 10.1021/acs.jafc.2c05747 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2023 Feb 1;71(4):1930-1943. doi: 10.1021/acs.jafc.2c05747. 
      Epub 2023 Jan 18.