PMID- 36656019
OWN - NLM
STAT- MEDLINE
DCOM- 20230208
LR  - 20230208
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 14
IP  - 3
DP  - 2023 Feb 6
TI  - beta(2 --> 1)-beta(2 --> 6) branched graminan-type fructans and beta(2 --> 1) linear fructans 
      impact mucus-related and endoplasmic reticulum stress-related genes in goblet 
      cells and attenuate inflammatory responses in a fructan dependent fashion.
PG  - 1338-1348
LID - 10.1039/d2fo02710k [doi]
AB  - Dietary fibers such as fructans have beneficial effects on intestinal health but 
      it is unknown whether they impact goblet cells (GCs). Here we studied the effects 
      of inulin-type fructans (ITFs) and graminan-type fructans (GTFs) with different 
      molecular weights on mucus- and endoplasmic reticulum (ER) stress-related genes 
      in intestinal GCs. To that end, GCs were incubated in the presence of ITFs or 
      GTFs, or ITFs and GTFs + TNFalpha or the N-glycosylation inhibitor tunicamycin (Tm). 
      IL-8 production by GCs was studied as a marker of inflammation. Effects between 
      ITFs and GTFs were compared. We found a beneficial impact of GTFs especially on 
      the expression of RETNLB. GTF II protects from the TNFalpha-induced gene expression 
      dysregulation of MUC2, TFF3, GAL3ST2, and CHST5. Also, all the studied fructans 
      prevented Tm-induced dysregulation of GAL3ST2. Interestingly, only the short 
      chain fructans ITF I and GTF I have anti-inflammatory properties on GCs. All the 
      studied fructans except ITF I decreased the expression of the ER stress-related 
      HSPA5 and XBP1. All these benefits were fructan-structure and chain length 
      dependent. Our study contributes to a better understanding of chemical 
      structure-dependent beneficial effects of ITFs and GTFs on gut barrier function, 
      which could contribute to prevention of gut inflammatory disorders.
FAU - Fernandez-Lainez, Cynthia
AU  - Fernandez-Lainez C
AUID- ORCID: 0000-0001-7468-3420
AD  - Immunoendocrinology, Division of Medical Biology, Department of Pathology and 
      Medical Biology, University Medical Centre Groningen, Hanzeplein 1, 9713 GZ, 
      Groningen, The Netherlands. c.fernandez.lainez@umcg.nl.
AD  - Laboratorio de Errores innatos del Metabolismo y Tamiz, Instituto Nacional de 
      Pediatria, Av. Iman 1, 04530, Ciudad de Mexico, Mexico.
AD  - Posgrado en Ciencias Biologicas, Universidad Nacional Autonoma de Mexico. 
      Edificio D, 1 degrees  Piso. Circuito de Posgrados, Ciudad Universitaria, 04510, Ciudad 
      de Mexico, Mexico.
FAU - Aan de Stegge, Myrthe
AU  - Aan de Stegge M
AUID- ORCID: 0000-0003-3668-8575
AD  - Immunoendocrinology, Division of Medical Biology, Department of Pathology and 
      Medical Biology, University Medical Centre Groningen, Hanzeplein 1, 9713 GZ, 
      Groningen, The Netherlands. c.fernandez.lainez@umcg.nl.
FAU - Silva-Lagos, Luis Alfredo
AU  - Silva-Lagos LA
AUID- ORCID: 0000-0002-2883-378X
AD  - Immunoendocrinology, Division of Medical Biology, Department of Pathology and 
      Medical Biology, University Medical Centre Groningen, Hanzeplein 1, 9713 GZ, 
      Groningen, The Netherlands. c.fernandez.lainez@umcg.nl.
FAU - Lopez-Velazquez, Gabriel
AU  - Lopez-Velazquez G
AD  - Laboratorio de Biomoleculas y Salud Infantil, Instituto Nacional de Pediatria, 
      Av. Iman 1, 04530, Cuidad de Mexico, Mexico. glv_1999@ciencias.unam.mx.
FAU - de Vos, Paul
AU  - de Vos P
AUID- ORCID: 0000-0001-9618-2408
AD  - Immunoendocrinology, Division of Medical Biology, Department of Pathology and 
      Medical Biology, University Medical Centre Groningen, Hanzeplein 1, 9713 GZ, 
      Groningen, The Netherlands. c.fernandez.lainez@umcg.nl.
LA  - eng
PT  - Journal Article
DEP - 20230206
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
RN  - 0 (Fructans)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9005-80-5 (Inulin)
SB  - IM
MH  - *Fructans/pharmacology/chemistry
MH  - *Goblet Cells/metabolism
MH  - Tumor Necrosis Factor-alpha/genetics
MH  - Mucus/metabolism
MH  - Inulin
MH  - Endoplasmic Reticulum Stress
EDAT- 2023/01/20 06:00
MHDA- 2023/02/09 06:00
CRDT- 2023/01/19 09:04
PHST- 2023/01/20 06:00 [pubmed]
PHST- 2023/02/09 06:00 [medline]
PHST- 2023/01/19 09:04 [entrez]
AID - 10.1039/d2fo02710k [doi]
PST - epublish
SO  - Food Funct. 2023 Feb 6;14(3):1338-1348. doi: 10.1039/d2fo02710k.