PMID- 36658478
OWN - NLM
STAT- MEDLINE
DCOM- 20230124
LR  - 20230124
IS  - 1689-1392 (Electronic)
IS  - 1425-8153 (Print)
IS  - 1425-8153 (Linking)
VI  - 28
IP  - 1
DP  - 2023 Jan 19
TI  - Photobiomodulation promotes spinal cord injury repair by inhibiting macrophage 
      polarization through lncRNA TUG1-miR-1192/TLR3 axis.
PG  - 5
LID - 10.1186/s11658-023-00417-0 [doi]
LID - 5
AB  - BACKGROUND: Secondary spinal cord injury (SCI) often causes the aggravation of 
      inflammatory reaction and nerve injury, which affects the recovery of motor 
      function. Bone-marrow-derived macrophages (BMDMs) were recruited to the injured 
      area after SCI, and the M1 polarization is the key process for inducing 
      inflammatory response and neuronal apoptosis. We previously showed that 
      photobiomodulation (PBM) can inhibit the polarization of M1 phenotype of BMDMs 
      and reduce inflammation, but the underlying mechanisms are unclear. The purpose 
      of this study is to explore the potential target and mechanism of PBM in treating 
      SCI. METHODS: Transcriptome sequencing and bioinformatics analysis showed that 
      long noncoding RNA taurine upregulated gene 1 (lncRNA TUG1) was a potential 
      target of PBM. The expression and specific mechanism of lncRNA TUG1 were detected 
      by qPCR, immunofluorescence, flow cytometry, western blotting, fluorescence 
      in situ hybridization, and luciferase assay. The Basso mouse scale (BMS) and gait 
      analysis were used to evaluate the recovery of motor function in mice. RESULTS: 
      Results showed that lncRNA TUG1 may be a potential target of PBM, regulating the 
      polarization of BMDMs, inflammatory response, and the axial growth of DRG. 
      Mechanistically, TUG1 competed with TLR3 for binding to miR-1192 and attenuated 
      the inhibitory effect of miR-1192 on TLR3. This effect protected TLR3 from 
      degradation, enabling the high expression of TLR3, which promoted the activation 
      of downstream NF-kappaB signal and the release of inflammatory cytokines. In vivo, 
      PBM treatment could reduce the expression of TUG1, TLR3, and inflammatory 
      cytokines and promoted nerve survival and motor function recovery in SCI mice. 
      CONCLUSIONS: Our study clarified that the lncRNA TUG1/miR-1192/TLR3 axis is an 
      important pathway for PBM to inhibit M1 macrophage polarization and inflammation, 
      which provides theoretical support for its clinical application in patients with 
      SCI.
CI  - (c) 2023. The Author(s).
FAU - Ju, Cheng
AU  - Ju C
AD  - Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, 
      Changle West Road No. 127, Xi'an, 710032, Shaanxi, China.
FAU - Ma, Yangguang
AU  - Ma Y
AD  - Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, 
      Changle West Road No. 127, Xi'an, 710032, Shaanxi, China.
FAU - Zuo, Xiaoshuang
AU  - Zuo X
AD  - Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, 
      Changle West Road No. 127, Xi'an, 710032, Shaanxi, China.
FAU - Wang, Xuankang
AU  - Wang X
AD  - Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, 
      Changle West Road No. 127, Xi'an, 710032, Shaanxi, China.
FAU - Song, Zhiwen
AU  - Song Z
AD  - Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, 
      Changle West Road No. 127, Xi'an, 710032, Shaanxi, China.
FAU - Zhang, Zhihao
AU  - Zhang Z
AD  - Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, 
      Changle West Road No. 127, Xi'an, 710032, Shaanxi, China.
FAU - Zhu, Zhijie
AU  - Zhu Z
AD  - Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, 
      Changle West Road No. 127, Xi'an, 710032, Shaanxi, China.
FAU - Li, Xin
AU  - Li X
AD  - Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, 
      Changle West Road No. 127, Xi'an, 710032, Shaanxi, China.
FAU - Liang, Zhuowen
AU  - Liang Z
AD  - Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, 
      Changle West Road No. 127, Xi'an, 710032, Shaanxi, China.
FAU - Ding, Tan
AU  - Ding T
AD  - Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, 
      Changle West Road No. 127, Xi'an, 710032, Shaanxi, China.
FAU - Hu, Xueyu
AU  - Hu X
AD  - Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, 
      Changle West Road No. 127, Xi'an, 710032, Shaanxi, China. huxueyu@fmmu.edu.cn.
FAU - Wang, Zhe
AU  - Wang Z
AD  - Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, 
      Changle West Road No. 127, Xi'an, 710032, Shaanxi, China. wangzhe@fmmu.edu.cn.
LA  - eng
GR  - NO.81070996/National Natural Scientific Foundation of China/
GR  - NO.81572151/National Natural Scientific Foundation of China/
GR  - NO.2020ZDLSF02-05/Shaanxi Provincial Key R&D Program/
GR  - NO.2021ZDLSF02-10/Shaanxi Provincial Key R&D Program/
GR  - 2018RCFC02/The Everest Project of Fourth Military Medical University/
GR  - XJZT19Z22/Discipline Boost Project of the First Affiliated Hospital of Air Force 
      Military Medical University/
GR  - XJZT21L01/Discipline Boost Project of the First Affiliated Hospital of Air Force 
      Military Medical University/
PT  - Journal Article
DEP - 20230119
PL  - England
TA  - Cell Mol Biol Lett
JT  - Cellular & molecular biology letters
JID - 9607427
RN  - 0 (Cytokines)
RN  - 0 (MicroRNAs)
RN  - 0 (MIRN1192 microRNA, mouse)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (TLR3 protein, mouse)
RN  - 0 (Toll-Like Receptor 3)
RN  - 0 (TUG1 noncoding RNA, mouse)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Cytokines/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Inflammation/genetics/metabolism
MH  - Macrophages/metabolism
MH  - *MicroRNAs/genetics/metabolism
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - *Spinal Cord Injuries/genetics
MH  - *Toll-Like Receptor 3/genetics
PMC - PMC9854040
OTO - NOTNLM
OT  - Bone-marrow-derived macrophages
OT  - Inflammation
OT  - Long noncoding RNA TUG1
OT  - Photobiomodulation
OT  - Spinal cord injury
OT  - Transcriptome sequencing
COIS- The authors declare no competing interests.
EDAT- 2023/01/20 06:00
MHDA- 2023/01/24 06:00
PMCR- 2023/01/19
CRDT- 2023/01/19 23:43
PHST- 2022/11/09 00:00 [received]
PHST- 2023/01/05 00:00 [accepted]
PHST- 2023/01/19 23:43 [entrez]
PHST- 2023/01/20 06:00 [pubmed]
PHST- 2023/01/24 06:00 [medline]
PHST- 2023/01/19 00:00 [pmc-release]
AID - 10.1186/s11658-023-00417-0 [pii]
AID - 417 [pii]
AID - 10.1186/s11658-023-00417-0 [doi]
PST - epublish
SO  - Cell Mol Biol Lett. 2023 Jan 19;28(1):5. doi: 10.1186/s11658-023-00417-0.