PMID- 36658569
OWN - NLM
STAT- MEDLINE
DCOM- 20230124
LR  - 20230210
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 19
IP  - 1
DP  - 2023 Jan 19
TI  - Optimized protocol for double vaccine immunization against classical swine fever 
      and porcine reproductive and respiratory syndrome.
PG  - 14
LID - 10.1186/s12917-022-03559-z [doi]
LID - 14
AB  - BACKGROUND: Classical swine fever and porcine reproductive and respiratory 
      syndrome have seriously affected the development of the swine breeding industry 
      in China. Vaccine immunization remains the main way to prevent these infections. 
      The aim of this study was to establish an optimized protocol for vaccine 
      immunization against classical swine fever virus (CSFV) and porcine reproductive 
      and respiratory syndrome virus (PRRSV). METHODS: Blood samples were collected 
      from the anterior vena cava of pigs after immunization, and blood indices, 
      secreted levels of specific antibodies and neutralizing antibodies associated 
      with humoral immunity, the proliferation capacity of T lymphocytes as a measure 
      of cellular immunity, and secreted levels of IFN-gamma and TNF-alpha were determined. 
      RESULTS: The results showed that simultaneous immunization against CSFV and PRRSV 
      infections induced strong and specific humoral and T-cellular immune responses, 
      high levels of cytokine IFN-gamma secretion and delayed secretion of cytokine TNF-alpha. 
      Moreover, significantly higher lymphocyte percentages and red blood cell and 
      leukocyte counts were found in the group simultaneously immunized against CSFV 
      and PRRSV. However, no statistically significant differences were observed in 
      hemoglobin values, neutrophil counts, and median cell percentages among the 
      S + PRRS, PRRS-S, and S-PRRS groups. CONCLUSION: This study demonstrated that 
      simultaneous immunization against CSFV and PRRSV had the advantages of inducing a 
      rapid, enhanced, and long-lasting immune response. These findings provide a 
      theoretical basis for the establishment of a reasonable and optimized vaccine 
      immunization protocol against CSFV and PRRSV in combination with a variety of 
      other vaccine inoculations.
CI  - (c) 2023. The Author(s).
FAU - Liu, Ziyu
AU  - Liu Z
AD  - Key Laboratory of Livestock Infectious Disease, Ministry of Education, College of 
      Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 
      China, No.120, Dongling Road, Shenhe District, 110866, People's Republic of 
      China.
FAU - Shan, Baiqiang
AU  - Shan B
AD  - Key Laboratory of Livestock Infectious Disease, Ministry of Education, College of 
      Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 
      China, No.120, Dongling Road, Shenhe District, 110866, People's Republic of 
      China.
FAU - Ni, Chao
AU  - Ni C
AD  - Key Laboratory of Livestock Infectious Disease, Ministry of Education, College of 
      Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 
      China, No.120, Dongling Road, Shenhe District, 110866, People's Republic of 
      China.
FAU - Feng, Shouhua
AU  - Feng S
AD  - Key Laboratory of Livestock Infectious Disease, Ministry of Education, College of 
      Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 
      China, No.120, Dongling Road, Shenhe District, 110866, People's Republic of 
      China.
FAU - Liu, Wanting
AU  - Liu W
AD  - Key Laboratory of Livestock Infectious Disease, Ministry of Education, College of 
      Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 
      China, No.120, Dongling Road, Shenhe District, 110866, People's Republic of 
      China.
FAU - Wang, Xiaoli
AU  - Wang X
AD  - Key Laboratory of Livestock Infectious Disease, Ministry of Education, College of 
      Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 
      China, No.120, Dongling Road, Shenhe District, 110866, People's Republic of 
      China.
FAU - Wu, Hongtao
AU  - Wu H
AD  - The Preventive Center of Animal Disease of Liaoning Province, Liaoning 
      Agricultural Development Service Center, No.95, Renhe Road, Shenbei District, 
      Shenyang, 110164, People's Republic of China.
FAU - ZuofengYang
AU  - ZuofengYang
AD  - The Preventive Center of Animal Disease of Liaoning Province, Liaoning 
      Agricultural Development Service Center, No.95, Renhe Road, Shenbei District, 
      Shenyang, 110164, People's Republic of China.
FAU - Liu, Jinling
AU  - Liu J
AD  - Key Laboratory of Livestock Infectious Disease, Ministry of Education, College of 
      Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 
      China, No.120, Dongling Road, Shenhe District, 110866, People's Republic of 
      China. liujl@syau.edu.cn.
FAU - Wei, Shu
AU  - Wei S
AD  - The Preventive Center of Animal Disease of Liaoning Province, Liaoning 
      Agricultural Development Service Center, No.95, Renhe Road, Shenbei District, 
      Shenyang, 110164, People's Republic of China. wweisshu@sohu.com.
FAU - Wu, Changde
AU  - Wu C
AD  - Key Laboratory of Livestock Infectious Disease, Ministry of Education, College of 
      Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 
      China, No.120, Dongling Road, Shenhe District, 110866, People's Republic of 
      China. wuchangde@syau.edu.cn.
FAU - Liu, Lixia
AU  - Liu L
AD  - Key Laboratory of Livestock Infectious Disease, Ministry of Education, College of 
      Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 
      China, No.120, Dongling Road, Shenhe District, 110866, People's Republic of 
      China. 1990500015@syau.edu.cn.
FAU - Chen, Zeliang
AU  - Chen Z
AD  - Key Laboratory of Livestock Infectious Disease, Ministry of Education, College of 
      Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 
      China, No.120, Dongling Road, Shenhe District, 110866, People's Republic of 
      China.
LA  - eng
PT  - Journal Article
DEP - 20230119
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Cytokines)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Viral Vaccines)
SB  - IM
EIN - BMC Vet Res. 2023 Feb 9;19(1):38. PMID: 36759813
MH  - Animals
MH  - Antibodies, Neutralizing
MH  - Antibodies, Viral
MH  - *Classical Swine Fever/prevention & control
MH  - Classical Swine Fever Virus
MH  - Cytokines
MH  - *Porcine Reproductive and Respiratory Syndrome/prevention & control
MH  - Porcine respiratory and reproductive syndrome virus
MH  - Swine
MH  - *Swine Diseases/prevention & control
MH  - Tumor Necrosis Factor-alpha
MH  - Vaccination/methods/veterinary
MH  - *Viral Vaccines
PMC - PMC9850545
OTO - NOTNLM
OT  - Cellular immunity
OT  - Classical swine fever
OT  - Humoral immunity
OT  - Porcine reproductive and respiratory syndrome
OT  - Vaccine
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as no potential 
      conflict of interest and competing interests.
EDAT- 2023/01/20 06:00
MHDA- 2023/01/24 06:00
PMCR- 2023/01/19
CRDT- 2023/01/19 23:47
PHST- 2022/05/19 00:00 [received]
PHST- 2022/12/21 00:00 [accepted]
PHST- 2023/01/19 23:47 [entrez]
PHST- 2023/01/20 06:00 [pubmed]
PHST- 2023/01/24 06:00 [medline]
PHST- 2023/01/19 00:00 [pmc-release]
AID - 10.1186/s12917-022-03559-z [pii]
AID - 3559 [pii]
AID - 10.1186/s12917-022-03559-z [doi]
PST - epublish
SO  - BMC Vet Res. 2023 Jan 19;19(1):14. doi: 10.1186/s12917-022-03559-z.