PMID- 36660634
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230121
IS  - 2305-5839 (Print)
IS  - 2305-5847 (Electronic)
IS  - 2305-5839 (Linking)
VI  - 10
IP  - 24
DP  - 2022 Dec
TI  - Transcriptome sequencing analysis of the role of beta-catenin in F-actin 
      reorganization in embryonic palatal mesenchymal cells.
PG  - 1332
LID - 10.21037/atm-22-5772 [doi]
LID - 1332
AB  - BACKGROUND: Palatogenesis is a highly regulated and coordinated developmental 
      process that is coordinated by multiple transcription factors and signaling 
      pathways. Our previous studies identified that defective palatal shelf 
      reorientation due to aberrant mesenchymal beta-catenin signaling is associated with 
      Filamentous actin (F-actin) dysregulation. Herein, the underlying mechanism of 
      mesenchymal beta-catenin in regulating F-actin cytoskeleton reorganization is 
      further investigated. METHODS: Firstly, beta-catenin silenced and overexpressed 
      mouse embryonic palatal mesenchymal (MEPM) cells were established by 
      adenovirus-mediated transduction. Subsequently, we compared transcriptomes of 
      negative control (NC) group, beta-catenin knockdown (KD) group, or beta-catenin 
      overexpression group respectively using RNA-sequencing (RNA-seq), and 
      differentially expressed genes (DEGs) screened were further identified by 
      quantitative real-time polymerase chain reaction (qRT-PCR). Finally, in vivo 
      experiments further verified the expression change of critical molecules. 
      RESULTS: We discovered 184 and 522 DEGs in the knockdown and overexpression 
      groups compared to the NC group, respectively (adjusted P<0.05; |fold change| 
      >2.0). Among these, 106 DEGs were altered in both groups. Gene Ontology (GO) 
      enrichment analysis relating to biological functions identified cytokine-cytokine 
      receptor interaction, and positive modulation of cellular migration. Kyoto 
      Encyclopedia of Genes and Genomes (KEGG) pathway enrichment assessment indicated 
      that these DEGs were chiefly linked by the regulation of signaling receptor 
      activity and chemokine signaling pathways. Quantitative real-time polymerase 
      chain reaction (qRT-PCR) results showed that the similar expression trend of 
      serum amyloid A3 (Saa3) and CXC-chemokine ligand 5 (Cxcl5) possibly involved in 
      cytoskeletal rearrangement with RNA-seq data. Experiments in vivo displayed that 
      no significant expression change of Saa3 and Cxcl5 was observed in beta-catenin 
      knockout and overexpressed mouse models. CONCLUSIONS: Our study provides an 
      expression landscape of DEGs in beta-catenin silenced and overexpressed MEPM cells, 
      which emphasizes the important role of processes such as chemotactic factor and 
      cell migration. Our data gain deeper insight into genes associated with F-actin 
      reorganization that is regulated by beta-catenin either directly or by another 
      route, which will contribute to further investigation of the exact mechanism of 
      mesenchymal beta-catenin/F-actin in palatal shelf reorientation.
CI  - 2022 Annals of Translational Medicine. All rights reserved.
FAU - Liu, Weilong
AU  - Liu W
AD  - Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, 
      Medical School of Nanjing University, Nanjing, China.
FAU - Lu, Yong
AU  - Lu Y
AD  - Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, 
      Medical School of Nanjing University, Nanjing, China.
FAU - Shi, Bing
AU  - Shi B
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases & Department of Cleft Lip and Palate Surgery, West China School of 
      Stomatology, Sichuan University, Chengdu, China.
FAU - Li, Chenghao
AU  - Li C
AD  - State Key Laboratory of Oral Diseases & National Clinical Research Center for 
      Oral Diseases & Department of Cleft Lip and Palate Surgery, West China School of 
      Stomatology, Sichuan University, Chengdu, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Ann Transl Med
JT  - Annals of translational medicine
JID - 101617978
PMC - PMC9843408
OTO - NOTNLM
OT  - F-actin reorganization
OT  - Palatogenesis
OT  - RNA-sequencing (RNA-seq)
OT  - palatal mesenchymal cells
OT  - beta-catenin
COIS- Conflicts of Interest: All authors have completed the ICMJE uniform disclosure 
      form (available at 
      https://atm.amegroups.com/article/view/10.21037/atm-22-5772/coif). The authors 
      have no conflicts of interest to declare.
EDAT- 2023/01/21 06:00
MHDA- 2023/01/21 06:01
PMCR- 2022/12/01
CRDT- 2023/01/20 02:16
PHST- 2022/10/28 00:00 [received]
PHST- 2022/12/07 00:00 [accepted]
PHST- 2023/01/20 02:16 [entrez]
PHST- 2023/01/21 06:00 [pubmed]
PHST- 2023/01/21 06:01 [medline]
PHST- 2022/12/01 00:00 [pmc-release]
AID - atm-10-24-1332 [pii]
AID - 10.21037/atm-22-5772 [doi]
PST - ppublish
SO  - Ann Transl Med. 2022 Dec;10(24):1332. doi: 10.21037/atm-22-5772.