PMID- 36661748
OWN - NLM
STAT- MEDLINE
DCOM- 20230406
LR  - 20230406
IS  - 1097-0010 (Electronic)
IS  - 0022-5142 (Linking)
VI  - 103
IP  - 7
DP  - 2023 May
TI  - Purification and characterization of the dipeptidyl peptidase-IV inhibitory 
      peptides from eel (Anguilla rostrata) scraps enzymatic hydrolysate for the 
      treatment of type 2 diabetes mellitus.
PG  - 3714-3724
LID - 10.1002/jsfa.12462 [doi]
AB  - BACKGROUND: Type 2 diabetes mellitus (T2DM) is a serious threat to human health. 
      Owing to the action of dipeptidyl peptidase-IV (DPP-IV), the half-life of 
      entero-insulin hormone after secretion is extremely short, causing insufficient 
      insulin secretion in diabetic patients. Dipeptidyl peptidase-IV inhibitors can be 
      used as a new treatment for T2DM. In this study, the proteins of eel (Anguilla 
      rostrata) scraps hydrolyzed using Protamex protease (EPHs) were found to have 
      strong DPP-IV inhibitory activity. The study also provided research ideas for the 
      development and utilization of A. rostrata scraps. RESULTS: The median inhibition 
      concentration (IC(50) ) value of EPHs was 5.455 +/- 0.24 mg mL(-1) . The peptide 
      fractions with the highest DPP-IV inhibitory activity were sequentially separated 
      by ultrafiltration, gel filtration chromatography (GFC), and reversed-phase high 
      performance liquid chromatography (RP-HPLC) in a continuous hierarchical manner 
      and analyzed using matrix-assisted laser desorption/ionization time-of-flight/ 
      time-of-flight mass spectrometry/mass spectrometry (MALDI-TOF/TOF MS/MS). Three 
      peptides that revealed significant inhibitory activity were screened among the 
      identified sequences, with sequences of Phe-Pro-Arg (IC(50)  = 62.14 +/- 1.47 muM), 
      Tyr-Pro-Pro-Ser-Phe-Ser (IC(50)  = 102.65 +/- 4.57 muM), and Tyr-Pro-Tyr-Pro-Ala-Ser 
      (IC(50)  = 68.30 +/- 3.85 muM). Molecular docking simulations revealed that their 
      inhibitory effect was mainly due to the formation of hydrogen bonds with amino 
      acid residues in the active sites of DPP-IV. Analysis of the inhibition patterns 
      of the synthetic peptides displayed that Phe-Pro-Arg and Tyr-Pro-Pro-Ser-Phe-Ser 
      displayed competitive inhibition, whereas Tyr-Pro-Tyr-Pro-Ala-Ser showed mixed 
      competitive/non-competitive inhibition. CONCLUSIONS: The protein hydrolysates 
      isolated from eel scraps are potential functional food ingredients for the 
      treatment of T2DM. (c) 2023 Society of Chemical Industry.
CI  - (c) 2023 Society of Chemical Industry.
FAU - Cao, Hongzhen
AU  - Cao H
AD  - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 
      China.
FAU - Di, Nana
AU  - Di N
AD  - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 
      China.
FAU - Jiang, Bo
AU  - Jiang B
AUID- ORCID: 0000-0002-0638-6456
AD  - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 
      China.
AD  - International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, China.
FAU - Chen, Jingjing
AU  - Chen J
AD  - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 
      China.
AD  - International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, China.
FAU - Zhang, Tao
AU  - Zhang T
AD  - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 
      China.
AD  - International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, China.
LA  - eng
GR  - Guangxi Zhongnan Fisheries Co., Ltd/
GR  - Chongzuo Scientific Research Project/
PT  - Journal Article
DEP - 20230205
PL  - England
TA  - J Sci Food Agric
JT  - Journal of the science of food and agriculture
JID - 0376334
RN  - 0 (Peptides)
RN  - EC 3.4.14.5 (Dipeptidyl Peptidase 4)
RN  - 0 (Dipeptidyl-Peptidase IV Inhibitors)
SB  - IM
MH  - Animals
MH  - Humans
MH  - *Anguilla/metabolism
MH  - *Diabetes Mellitus, Type 2/drug therapy/veterinary
MH  - Tandem Mass Spectrometry
MH  - Molecular Docking Simulation
MH  - Peptides/chemistry
MH  - Dipeptidyl Peptidase 4/chemistry
MH  - *Dipeptidyl-Peptidase IV Inhibitors/chemistry
OTO - NOTNLM
OT  - DPP-IV inhibitory peptides
OT  - T2DM
OT  - eel (Anguilla rostrata) scraps
OT  - hydrolysis
OT  - purification
EDAT- 2023/01/21 06:00
MHDA- 2023/04/06 06:42
CRDT- 2023/01/20 09:35
PHST- 2023/01/06 00:00 [revised]
PHST- 2022/09/01 00:00 [received]
PHST- 2023/01/20 00:00 [accepted]
PHST- 2023/04/06 06:42 [medline]
PHST- 2023/01/21 06:00 [pubmed]
PHST- 2023/01/20 09:35 [entrez]
AID - 10.1002/jsfa.12462 [doi]
PST - ppublish
SO  - J Sci Food Agric. 2023 May;103(7):3714-3724. doi: 10.1002/jsfa.12462. Epub 2023 
      Feb 5.